FastQCFastQC Report
Thu 26 May 2016
SRR1511850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802769
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51930.6468859659503543No Hit
TATCAACGCAGAGTACTTTTTTTTT40740.5074934383365576No Hit
GGTATCAACGCAGAGTACTTTTTTT39010.4859430296884907No Hit
CTGTAGGACGTGGAATATGGCAAGA26990.3362112886770665No Hit
CTTTAGGACGTGAAATATGGCGAGG21540.26832127299385No Hit
GTCCTAAAGTGTGTATTTCTCATTT21230.26445963907425424No Hit
GTCCTACAGTGGACATTTCTAAATT20490.2552415452016707No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20400.25412042567662674No Hit
CTGAAGGACCTGGAATATGGCGAGA14560.18137222538488657No Hit
TTTCTAAATTTTCCACCTTTTTCAG12840.15994638557293567No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12370.15409165027548397No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11970.14910889683084425No Hit
GTCCTACAGTGTGCATTTCTCATTT11790.14686665778075636No Hit
ATTTAGAAATGTCCACTGTAGGACG11740.14624381360017638No Hit
CTGTAGGACCTGGAATATGGCGAGA11610.14462441873066847No Hit
GAATATGGCAAGAAAACTGAAAATC11020.13727485739982484No Hit
GGAATATGGCGAGAAAACTGAAAAT9990.12444426727987751No Hit
ACAGTGGACATTTCTAAATTTTCCA8750.10899773160149434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAC307.7300385E-418.99570310
GGTATCA8400.014.747741
CCACCTT3850.014.06175413
TAGGACC5000.013.3003054
TCCACCT4200.013.1160812
TTCCACC4350.012.4454611
TGGTATC2000.012.3502842
TATCCAG701.0893996E-412.2145665
TTGGACT550.003065697412.0911864
TAAGCCA550.003065697412.0911864
TTTCCAC4350.012.00877810
TTATACT802.8616429E-511.8752734
CACCTTT4600.011.76907514
GTGGTAT1950.011.7283811
AGTTCAG658.0312387E-411.68966310
TGTGAAC752.0709915E-411.4002623
AAATGTC3750.011.1441457
AATGTCC3850.011.1013858
CTAAGCC600.00587046311.0835873
GGCAGCC600.00588136211.08082614
Produced by FastQC (version 0.11.5)