FastQCFastQC Report
Thu 26 May 2016
SRR1511849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870713
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64370.22423000836377582No Hit
GTCCTACAGTGGACATTTCTAAATT41190.14348351785775867No Hit
TATCAACGCAGAGTACTTTTTTTTT40860.14233397765642194No Hit
GGTATCAACGCAGAGTACTTTTTTT40340.1405225809755277No Hit
CTGTAGGACGTGGAATATGGCAAGA40240.1401742354599711No Hit
GTCCTAAAGTGTGTATTTCTCATTT36820.12826081882793577No Hit
CTTTAGGACGTGAAATATGGCGAGG33080.11523269654611938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.013.924391
TAGGACC8350.012.7816874
GGCGAGG10950.011.38639519
TCCAACG3850.011.28557118
TCGCATT600.00613465911.01978818
TACGGTG600.0061378411.0190195
GGACGTA700.001566271810.7941426
CCAACGA2400.010.62622419
TGGCGAG23750.010.499454518
AGGACGT25450.010.465445
GGACGTG24650.010.4219296
ACTGTTC8450.010.3949518
TTAGGAC16300.010.3719793
GTAGGAC26750.010.2039953
GACGTGA12800.010.1827557
GATATAC7400.010.1296811
TGTAGGA26400.010.1081932
CAAGTCG2350.010.0477386
GTATCAA31050.010.0429011
GCGCGCC856.958986E-410.00045511
Produced by FastQC (version 0.11.5)