FastQCFastQC Report
Thu 26 May 2016
SRR1511849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870713
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85560.2980444231102169No Hit
GGTATCAACGCAGAGTACTTTTTTT56090.19538699967569031No Hit
TATCAACGCAGAGTACTTTTTTTTT55960.19493415050546675No Hit
GTCCTACAGTGGACATTTCTAAATT48080.1674845238796076No Hit
CTGTAGGACGTGGAATATGGCAAGA44040.15341136505112146No Hit
GTCCTAAAGTGTGTATTTCTCATTT40450.14090576104263994No Hit
CTTTAGGACGTGAAATATGGCGAGG37580.13090824474616586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.013.5727761
TAGGACC7550.012.5826214
TCCAACG2650.012.18533818
TAGCGCT550.003069829212.0907544
GGCGAGG9900.011.03232919
TTAGGAC14500.010.6136573
GTCCTAG3550.010.4795731
TACACCG1002.4006564E-510.4498665
GGACGTG24600.010.3851826
AGGACGT24850.010.3600285
GTCCTAC23600.010.3474971
ACGTGTC1201.5306323E-610.2886668
GACGTGA12350.010.1508877
GTAGGAC25250.010.0454163
TGGCGAG24050.09.99102318
AACAGTG9150.09.9643087
GAACAGT7150.09.962136
TAGGACG26900.09.9236644
TGTAGGA25850.09.9225032
GTATCAA33900.09.6797941
Produced by FastQC (version 0.11.5)