FastQCFastQC Report
Thu 26 May 2016
SRR1511848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081910
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61890.20081702580542587No Hit
GTCCTACAGTGGACATTTCTAAATT52820.17138722415644844No Hit
CTGTAGGACGTGGAATATGGCAAGA45310.1470192186014517No Hit
GTCCTAAAGTGTGTATTTCTCATTT44160.1432877663526839No Hit
CTTTAGGACGTGAAATATGGCGAGG41550.1348189921185239No Hit
TATCAACGCAGAGTACTTTTTTTTT38190.12391666206995013No Hit
GGTATCAACGCAGAGTACTTTTTTT38130.12372197760479701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2250.014.692518
CGGTAGG1151.6916601E-1013.9049951
GGTATCA15950.012.7825691
TAGGACC10850.011.8391174
CCAACGA2000.011.33421419
GGCGAGG12200.010.99356819
GTCCTAA16050.010.9593881
GCCGGTT1658.913048E-1110.87608911
ATACTCG700.001566119510.7943136
CGTTAGG650.00730980710.76300051
GTAGGAC31950.010.6720173
TGTAGGA31500.010.6604962
TATTCCG803.9801383E-410.6258255
GTGCGAT803.9807346E-410.62565311
CTGTAGG31050.010.3321831
AGGACGT29050.010.1767335
GTATCAA31750.010.1043871
GGACGTG28450.010.05926
TAGACGT856.9572334E-410.0007774
GACGTGG15300.010.0004527
Produced by FastQC (version 0.11.5)