##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511848_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3081910 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.23741024234971 31.0 30.0 34.0 24.0 34.0 2 26.51418178986408 31.0 26.0 34.0 10.0 34.0 3 28.249183460905737 31.0 28.0 34.0 16.0 34.0 4 32.58701908881181 35.0 32.0 37.0 25.0 37.0 5 32.94223030523279 35.0 35.0 37.0 26.0 37.0 6 33.13502665554802 37.0 35.0 37.0 27.0 37.0 7 33.112523078220974 37.0 35.0 37.0 26.0 37.0 8 33.1255280004932 37.0 35.0 37.0 26.0 37.0 9 34.64775090771632 39.0 35.0 39.0 27.0 39.0 10 34.52103792777855 39.0 35.0 39.0 27.0 39.0 11 34.52199058376137 39.0 35.0 39.0 27.0 39.0 12 34.395664376961044 39.0 35.0 39.0 26.0 39.0 13 34.385122862121214 39.0 35.0 39.0 26.0 39.0 14 35.454994467716446 40.0 36.0 41.0 26.0 41.0 15 35.39431716046218 40.0 36.0 41.0 26.0 41.0 16 35.33924189869269 40.0 36.0 41.0 25.0 41.0 17 35.28332527555963 40.0 36.0 41.0 25.0 41.0 18 35.252794208786106 40.0 36.0 41.0 25.0 41.0 19 35.26884691636031 40.0 36.0 41.0 25.0 41.0 20 35.196918793864846 40.0 36.0 41.0 25.0 41.0 21 35.08410109315327 40.0 35.0 41.0 24.0 41.0 22 34.981461496279906 40.0 35.0 41.0 24.0 41.0 23 34.86220947399502 39.0 35.0 41.0 23.0 41.0 24 34.74241395757826 39.0 35.0 41.0 21.0 41.0 25 34.63243443189451 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7500631309630599 1101 2 3.221079110198957 1101 3 1.9081476851799515 1101 4 0.9274265761384584 1101 5 0.3514298334698296 1101 6 0.43451424617089174 1101 7 0.1649923184714268 1101 8 0.24510201942076293 1101 9 0.2719216039676766 1101 10 0.2739798883024491 1101 11 0.22501041390817988 1101 12 0.14635100954853186 1101 13 0.16307529405835197 1101 14 0.15086541314405366 1101 15 0.3358396343836958 1101 16 0.3388331234273565 1101 17 0.17768298365933788 1101 18 0.333413563996686 1101 19 0.4652800781761286 1101 20 0.45098038657040007 1101 21 0.4372100838090347 1101 22 0.281232174856612 1101 23 0.29781223684172886 1101 24 0.41113069768181987 1101 25 0.4207653091661925 1102 1 1.2821289149312314 1102 2 3.9273070061408397 1102 3 2.2761995167584175 1102 4 1.4712689167532957 1102 5 1.1627198894463149 1102 6 0.9683614474282223 1102 7 0.855372665109968 1102 8 0.728915970828524 1102 9 0.9014094471789562 1102 10 0.9392106378942557 1102 11 0.8327781133840944 1102 12 1.0066841844972956 1102 13 0.9660346124991719 1102 14 1.1930108931011318 1102 15 1.1103322809571594 1102 16 1.0073064834514938 1102 17 1.2483679632921039 1102 18 1.2100667143312833 1102 19 1.2343106391248213 1102 20 1.1872861673526955 1102 21 1.2393764792580342 1102 22 1.2634640989673045 1102 23 1.2392925015967222 1102 24 1.0250404817569176 1102 25 1.1607711244803198 1103 1 1.15701670529047 1103 2 3.7297387545039875 1103 3 2.2495968223641434 1103 4 1.498174069367579 1103 5 0.9838663056964521 1103 6 0.8389600447454626 1103 7 0.8335038087925639 1103 8 0.7147850913382001 1103 9 0.7197066231260294 1103 10 1.064530034036686 1103 11 1.1062830354067472 1103 12 0.9338857147157285 1103 13 1.1012512593383121 1103 14 1.1990951782998422 1103 15 0.9845666672067921 1103 16 1.0305298493711632 1103 17 1.1062882144425075 1103 18 1.140315366963783 1103 19 1.1079365706450304 1103 20 1.1229907311956282 1103 21 1.1423419065012368 1103 22 1.0786144251582783 1103 23 1.202424443360151 1103 24 1.2358015730492369 1103 25 1.2226001108285516 1104 1 1.15063555760171 1104 2 3.6965624346714954 1104 3 2.070703047658217 1104 4 1.3467213477792228 1104 5 1.1210271929702387 1104 6 0.7951910500075741 1104 7 0.7161981826561572 1104 8 0.7714938947097423 1104 9 0.9256480977115018 1104 10 0.884143282072877 1104 11 0.8957729898962867 1104 12 0.870897127759072 1104 13 0.9293463126439789 1104 14 1.0480910306925253 1104 15 0.9649227073600883 1104 16 1.0534445666069203 1104 17 1.003438193728421 1104 18 1.0511836665002576 1104 19 1.0522427951322229 1104 20 1.0372820864261243 1104 21 1.2204597163003257 1104 22 0.9811558653001242 1104 23 1.1650519774312187 1104 24 1.1526900682486882 1104 25 1.0911731034473817 1105 1 1.2160608845884227 1105 2 3.8518046799774517 1105 3 2.123164220209887 1105 4 1.4509960762060103 1105 5 1.0665194359240715 1105 6 0.8100051759397644 1105 7 0.7834922980894561 1105 8 0.7088301962962333 1105 9 0.8624145834700911 1105 10 0.8084484843080446 1105 11 0.9436336595529156 1105 12 0.767498593695926 1105 13 0.8174013855126177 1105 14 0.9847289339632752 1105 15 0.941497052765456 1105 16 0.9172220354328999 1105 17 0.9857321680346018 1105 18 0.9792822933695575 1105 19 0.9240701790527552 1105 20 0.9019257380336825 1105 21 1.0617547904330706 1105 22 0.9396741415035095 1105 23 0.9800221392980006 1105 24 0.9922210533714804 1105 25 1.075141906875153 1106 1 0.9334870742385171 1106 2 3.5149477201726533 1106 3 1.9017588712525892 1106 4 1.0408565499339346 1106 5 0.7115092172499544 1106 6 0.5946847438628069 1106 7 0.5732194425751942 1106 8 0.3273470160004237 1106 9 0.4611738137795669 1106 10 0.533566675016587 1106 11 0.6016078313394075 1106 12 0.40013548891839434 1106 13 0.3168176111359102 1106 14 0.39899592341048873 1106 15 0.4704611522148028 1106 16 0.5648529630965413 1106 17 0.5514280278784014 1106 18 0.5495035847697451 1106 19 0.5273099957171823 1106 20 0.5552564645757769 1106 21 0.5234088650392295 1106 22 0.46444416560714785 1106 23 0.42089361006587467 1106 24 0.5314954842373609 1106 25 0.47380735564055954 1107 1 1.185107283765909 1107 2 3.641074406571285 1107 3 2.2095259982038726 1107 4 1.4038106123263674 1107 5 1.131339588868677 1107 6 0.9973328199959539 1107 7 0.9291477983853085 1107 8 0.9273329400182746 1107 9 0.8916037838489146 1107 10 0.9610784380589479 1107 11 0.8945685606288407 1107 12 0.9449150270470312 1107 13 1.0023567148541446 1107 14 1.1733503796744955 1107 15 1.1486212421987219 1107 16 1.0330317741367168 1107 17 1.022537202475604 1107 18 0.9688397614514699 1107 19 0.9739508905401593 1107 20 1.0895097273244616 1107 21 0.953959376701178 1107 22 1.1081878394128992 1107 23 1.1099412163171465 1107 24 1.132664430573314 1107 25 1.1834996930597654 1108 1 1.0011678261929795 1108 2 3.675531434177735 1108 3 2.107450671782324 1108 4 1.3190284247444666 1108 5 0.9005588630823098 1108 6 0.743085229598222 1108 7 0.6847359106342736 1108 8 0.6877387917583491 1108 9 0.651469845612219 1108 10 0.6365559226637032 1108 11 0.702965257426726 1108 12 0.7073553465584155 1108 13 0.7122199279067729 1108 14 0.9242337241584906 1108 15 0.7620808303891025 1108 16 0.852328126322007 1108 17 0.7626198639201718 1108 18 0.8326739957912963 1108 19 0.8222972546900351 1108 20 0.8131180507686935 1108 21 0.6797952291086631 1108 22 0.7049637500643087 1108 23 0.8358812501435509 1108 24 0.8678144273990398 1108 25 0.9913494374083314 1109 1 0.5254996233616325 1109 2 2.475975279093099 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-1.523572683380813 2315 14 -1.610526270813196 2315 15 -1.623131712339429 2315 16 -1.6538339689354302 2315 17 -1.4260257080257333 2315 18 -1.4914941576107879 2315 19 -1.566238685627816 2315 20 -1.6139466983718975 2315 21 -1.671655727962154 2315 22 -1.6517947315799475 2315 23 -1.753803948740547 2315 24 -1.9126056297496206 2315 25 -1.8369017991203975 2316 1 -1.5986369407121863 2316 2 -1.3073163166196125 2316 3 -0.868344337816275 2316 4 -1.1674717502696126 2316 5 -1.3080205226508888 2316 6 -1.4957797225827107 2316 7 -1.4203603688490745 2316 8 -1.561821107624862 2316 9 -1.573881584828925 2316 10 -1.8259902386579725 2316 11 -1.8847517545938075 2316 12 -1.8341363821241075 2316 13 -1.7206507160363316 2316 14 -1.9747772832486277 2316 15 -2.2738953676963902 2316 16 -2.058311294674901 2316 17 -2.0076877477790305 2316 18 -2.0362986824458673 2316 19 -1.936996688518441 2316 20 -2.2183508282498323 2316 21 -2.1615587058817596 2316 22 -2.064774511491514 2316 23 -2.0947412564493675 2316 24 -2.0155288849978277 2316 25 -2.1406382224686595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 130614.0 3 4.0 4 1196.0 5 5998.0 6 7046.0 7 6217.0 8 5583.0 9 5458.0 10 5274.0 11 5097.0 12 4976.0 13 4979.0 14 4990.0 15 5245.0 16 5432.0 17 5933.0 18 6634.0 19 8405.0 20 10683.0 21 13439.0 22 16630.0 23 19901.0 24 23547.0 25 26654.0 26 29671.0 27 33370.0 28 38307.0 29 45657.0 30 56777.0 31 71493.0 32 91282.0 33 118393.0 34 159801.0 35 226289.0 36 344694.0 37 462452.0 38 1073789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95693255156704 18.87037583835998 13.34636637669497 24.826325233378004 2 15.981512070131082 20.770904925937383 40.39678596774088 22.850797036190652 3 18.870797654701143 24.27942412335208 29.51585218257509 27.333926039371686 4 12.213108105038758 16.801139553069362 38.44372483297695 32.54202750891493 5 13.265455116532312 37.75687142833039 35.73220606043301 13.245467394704292 6 31.708262015818296 37.05007503548976 18.2495396471304 12.99212330156155 7 28.149037447556875 31.396958379706092 22.068133073321412 18.38587109941562 8 24.811594108848084 37.28593631871145 20.550048508879236 17.352421063561234 9 26.14602632782917 15.579299849768487 20.476976939625104 37.79769688277724 10 14.957867036999783 29.18057308617059 34.39636459208737 21.465195284742254 11 34.045672975524916 22.559678900422142 24.715582220116747 18.679065903936195 12 23.298311761213014 24.5944235879698 31.829417471632855 20.277847179184334 13 28.81849738441082 22.076111341488026 25.885312530622283 23.220078743478872 14 20.726692213594816 22.443841643656047 27.776476276075552 29.052989866673588 15 22.773247758695096 31.27482632523338 23.39834712889085 22.55357878718068 16 21.988572021895514 28.344338413516297 28.003835283963518 21.663254280624678 17 21.550134203449314 28.804291395404373 27.97629245449543 21.669281946650884 18 21.344685600812483 27.671379112303736 30.015477414979674 20.96845787190411 19 23.333543160740557 27.0551783349092 27.715518164455798 21.89576033989444 20 23.353463811539267 27.409086749901196 28.352694803909547 20.88475463464999 21 22.9058088531618 26.865791746731382 27.3412804254024 22.887118974704418 22 22.916375978739488 28.18003939294626 27.54261349808714 21.36097113022711 23 22.248995019632364 27.81422894505696 27.933343738166798 22.003432297143874 24 22.873504066058935 26.900787642763092 28.63779400322014 21.587914287957833 25 22.629127267040076 27.384825246391653 28.273405665758776 21.71264182080949 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 180.0 1 180.0 2 386.5 3 593.0 4 593.0 5 593.0 6 1571.5 7 2550.0 8 2550.0 9 2550.0 10 3890.5 11 5231.0 12 5231.0 13 5231.0 14 9326.0 15 13421.0 16 13421.0 17 13421.0 18 24134.5 19 34848.0 20 34848.0 21 34848.0 22 56255.0 23 77662.0 24 77662.0 25 77662.0 26 112404.5 27 147147.0 28 147147.0 29 147147.0 30 192695.5 31 238244.0 32 238244.0 33 238244.0 34 270249.0 35 302254.0 36 302254.0 37 302254.0 38 325877.0 39 349500.0 40 349500.0 41 349500.0 42 368918.5 43 388337.0 44 388337.0 45 388337.0 46 396143.5 47 403950.0 48 403950.0 49 403950.0 50 389019.5 51 374089.0 52 374089.0 53 374089.0 54 332045.5 55 290002.0 56 290002.0 57 290002.0 58 247743.5 59 205485.0 60 205485.0 61 205485.0 62 167462.0 63 129439.0 64 129439.0 65 129439.0 66 99159.0 67 68879.0 68 68879.0 69 68879.0 70 50103.0 71 31327.0 72 31327.0 73 31327.0 74 21854.5 75 12382.0 76 12382.0 77 12382.0 78 8423.0 79 4464.0 80 4464.0 81 4464.0 82 2934.5 83 1405.0 84 1405.0 85 1405.0 86 888.0 87 371.0 88 371.0 89 371.0 90 240.0 91 109.0 92 109.0 93 109.0 94 70.5 95 32.0 96 32.0 97 32.0 98 20.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.493573790279404 3 0.0 4 0.0 5 5.840533954593092E-4 6 0.0011356593800597682 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.2978964343540207E-4 14 0.0 15 0.0 16 0.0 17 7.138430388947114E-4 18 0.0 19 3.893689303062063E-4 20 5.191585737416083E-4 21 9.085275040478146E-4 22 0.004542637520239073 23 2.920266977296546E-4 24 0.0018170550080956291 25 9.085275040478146E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3081910.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.37645416368305 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61600468199055 58.96837471370724 2 11.74885307022928 16.771829452861343 3 3.030729387352548 6.4896815159668915 4 1.1193826818206078 3.195902667223569 5 0.5111535168902632 1.8242162784461637 6 0.2653115085012177 1.1362196835380862 7 0.16146821775436662 0.8067520190405301 8 0.10646907479142259 0.6079508029359774 9 0.07303165228192234 0.4691466343438801 >10 0.33439633036927996 4.0495151946636385 >50 0.017176977008312385 0.8652193492320307 >100 0.012946612731439919 1.8322878241767309 >500 0.001698845767681565 0.8282548638339632 >1k 0.001285613010273485 1.7787177087066377 >5k 9.182950073382035E-5 0.375931291323332 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6189 0.20081702580542587 No Hit GTCCTACAGTGGACATTTCTAAATT 5282 0.17138722415644844 No Hit CTGTAGGACGTGGAATATGGCAAGA 4531 0.1470192186014517 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4416 0.1432877663526839 No Hit CTTTAGGACGTGAAATATGGCGAGG 4155 0.1348189921185239 No Hit TATCAACGCAGAGTACTTTTTTTTT 3819 0.12391666206995013 No Hit GGTATCAACGCAGAGTACTTTTTTT 3813 0.12372197760479701 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.244741085885052E-5 5 0.0 0.0 0.0 0.0 3.244741085885052E-5 6 0.0 0.0 0.0 0.0 3.244741085885052E-5 7 0.0 0.0 0.0 0.0 3.244741085885052E-5 8 0.0 0.0 0.0 0.0 3.244741085885052E-5 9 0.0 0.0 0.0 0.0 3.244741085885052E-5 10 0.0 0.0 0.0 0.0 3.244741085885052E-5 11 0.0 0.0 0.0 0.0 3.244741085885052E-5 12 0.0 0.0 0.0 0.0 3.244741085885052E-5 13 0.0 0.0 0.0 0.0 3.244741085885052E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 225 0.0 14.6925 18 CGGTAGG 115 1.6916601E-10 13.904995 1 GGTATCA 1595 0.0 12.782569 1 TAGGACC 1085 0.0 11.839117 4 CCAACGA 200 0.0 11.334214 19 GGCGAGG 1220 0.0 10.993568 19 GTCCTAA 1605 0.0 10.959388 1 GCCGGTT 165 8.913048E-11 10.876089 11 ATACTCG 70 0.0015661195 10.794313 6 CGTTAGG 65 0.007309807 10.7630005 1 GTAGGAC 3195 0.0 10.672017 3 TGTAGGA 3150 0.0 10.660496 2 TATTCCG 80 3.9801383E-4 10.625825 5 GTGCGAT 80 3.9807346E-4 10.625653 11 CTGTAGG 3105 0.0 10.332183 1 AGGACGT 2905 0.0 10.176733 5 GTATCAA 3175 0.0 10.104387 1 GGACGTG 2845 0.0 10.0592 6 TAGACGT 85 6.9572334E-4 10.000777 4 GACGTGG 1530 0.0 10.000452 7 >>END_MODULE