FastQCFastQC Report
Thu 26 May 2016
SRR1511848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081910
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84250.27336943648581563No Hit
GTCCTACAGTGGACATTTCTAAATT58570.19004448540028748No Hit
TATCAACGCAGAGTACTTTTTTTTT54300.17618944096355832No Hit
GGTATCAACGCAGAGTACTTTTTTT53300.17294469987767327No Hit
CTGTAGGACGTGGAATATGGCAAGA51590.16739619262080982No Hit
GTCCTAAAGTGTGTATTTCTCATTT46390.15052353897420756No Hit
CTTTAGGACGTGAAATATGGCGAGG44870.14559153252366228No Hit
GTCCTACAGTGTGCATTTCTCATTT32080.10409129403519246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.012.7846391
TAGGACC12400.011.7224054
TATGCCG752.07404E-411.4006135
GCCGGTT1850.011.2936911
AGGACGT29450.011.1618925
GGACGTG29250.010.9742736
GGCGAGG13300.010.92561319
TGGTATC6500.010.5236432
TAGGACG31550.010.4490574
TGTAGGA33650.010.4180942
TTAGGAC17700.010.4129913
GACGTGA15700.010.404327
TCCAACG2500.010.25722218
CGTGCGC1502.4598194E-810.13009710
GACGTGG15300.010.1176827
ATAGGAC3950.010.10180953
CTGTAGG33250.010.0991531
TGGCGAG28350.010.0836918
GTAGGAC32800.010.0508463
GTCCTAC29900.010.0501671
Produced by FastQC (version 0.11.5)