FastQCFastQC Report
Thu 26 May 2016
SRR1511847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154640
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75250.34924627780046785No Hit
GTCCTACAGTGGACATTTCTAAATT54860.2546132996695504No Hit
CTGTAGGACGTGGAATATGGCAAGA51280.23799799502469088No Hit
GGTATCAACGCAGAGTACTTTTTTT46220.21451379348754315No Hit
GTCCTAAAGTGTGTATTTCTCATTT44400.2060669067686481No Hit
TATCAACGCAGAGTACTTTTTTTTT44150.20490662013143726No Hit
CTTTAGGACGTGAAATATGGCGAGG40690.18884825307243902No Hit
GTCCTACAGTGTGCATTTCTCATTT28090.13036980655701183No Hit
CTGTAGGACCTGGAATATGGCGAGA24330.11291909553336056No Hit
CTGAAGGACCTGGAATATGGCGAGA22330.1036368024356737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAACG551.2731858E-414.5386322
GGTATCA11700.013.7542291
GGACGTA552.0598788E-413.7380156
CGTTAGG450.00822566613.327081
TAGGACC10200.013.2413594
TAGTACG500.001567084213.2228394
GTGGTAT4200.012.3751451
GGCGAGG12700.011.82497419
GCGCAGG709.754668E-411.423211
GACCTAC1851.8189894E-1211.3460261
GTATCAA26950.011.1635921
CTCGAAC1803.6379788E-1211.01928918
AGAACCG600.006136942711.0190335
GTCCTAC27500.010.9403211
TGTAGGA32250.010.8786152
GTACTAA1852.1827873E-1110.8057391
TCCAACG1855.456968E-1210.7214718
TGTCCAC9100.010.69036410
GTAGGAC32850.010.6380763
AGGACGT30550.010.6351585
Produced by FastQC (version 0.11.5)