FastQCFastQC Report
Thu 26 May 2016
SRR1511847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154640
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95870.4449467196376193No Hit
GGTATCAACGCAGAGTACTTTTTTT62570.2903965395611332No Hit
GTCCTACAGTGGACATTTCTAAATT58960.2736420005198084No Hit
TATCAACGCAGAGTACTTTTTTTTT58680.27234247948613227No Hit
CTGTAGGACGTGGAATATGGCAAGA56020.25999702966620875No Hit
GTCCTAAAGTGTGTATTTCTCATTT47930.22245015408606542No Hit
CTTTAGGACGTGAAATATGGCGAGG45930.21316786098837856No Hit
GTCCTACAGTGTGCATTTCTCATTT33160.153900419559648No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29440.13663535439795046No Hit
CTGTAGGACCTGGAATATGGCGAGA27410.12721382690379832No Hit
CTGAAGGACCTGGAATATGGCGAGA25480.1182564140645305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG400.005289402314.246179
GGTATCA17200.013.3059811
TAGGACC10400.012.8796354
TGTCGAG1001.4480429E-712.34696818
TATGACG752.0797085E-411.39693616
GGCGAGG12150.011.25649519
CCAACGA600.005887656511.08061319
AGGACCT17350.011.060375
GTCTTAG3050.010.9428961
CTAGTAC1658.0035534E-1110.9392473
TCCAACG1408.38736E-910.85447818
AGGACCG803.7724184E-410.6873565
GTAGGAC30850.010.5314633
GTGGTAT6100.010.4739141
TGTAGGA32850.010.3529822
GGACCTG17200.010.3257146
GACGTGG14500.010.2179437
AATGTCC7250.010.2179438
CTGTAGG32300.010.2149831
ATTGCGA750.002654191810.1306117
Produced by FastQC (version 0.11.5)