FastQCFastQC Report
Thu 26 May 2016
SRR1511846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472340
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67660.27366786121649933No Hit
GTCCTACAGTGGACATTTCTAAATT50080.20256113641327647No Hit
CTGTAGGACGTGGAATATGGCAAGA49270.19928488800084132No Hit
GTCCTAAAGTGTGTATTTCTCATTT49000.19819280519669624No Hit
GGTATCAACGCAGAGTACTTTTTTT44930.18173066811199107No Hit
CTTTAGGACGTGAAATATGGCGAGG44380.17950605499243633No Hit
TATCAACGCAGAGTACTTTTTTTTT44050.17817128712070346No Hit
GTCCTACAGTGTGCATTTCTCATTT26520.10726679987380376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.014.1698591
TGAGCCG701.1521386E-412.1436115
TAGGACC9350.012.1219644
AAAGCGT600.006136673511.0192026
TTAATCC3500.010.7943214
CCCTACA2500.010.7937362
CGTATGT650.007309151310.7629851
GGCGAGG12750.010.66754419
GGACCTG17300.010.53694256
TCCAACG1451.5805199E-810.42231418
CTAATAC2001.8189894E-1210.3895343
AGGACCT18550.010.3869885
AAGACTA2100.010.3445585
CTGTAGG29350.010.2155371
AGGACGT32000.010.212445
GACGTGG16050.010.1806267
TGTAGGA29500.010.1635942
GTCCTAA17300.010.0519711
GTAGGAC31900.010.0371963
GGACGTG32350.010.0143616
Produced by FastQC (version 0.11.5)