##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511846_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2472340 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.747330059781422 31.0 30.0 34.0 25.0 34.0 2 26.964898840774328 31.0 27.0 34.0 10.0 34.0 3 28.731901356609526 31.0 28.0 34.0 16.0 34.0 4 33.14995065403626 35.0 33.0 37.0 28.0 37.0 5 33.52714594271014 35.0 35.0 37.0 28.0 37.0 6 33.74106554923676 37.0 35.0 37.0 29.0 37.0 7 33.73382463577016 37.0 35.0 37.0 29.0 37.0 8 33.75628352087496 37.0 35.0 37.0 29.0 37.0 9 35.33198467848273 39.0 35.0 39.0 30.0 39.0 10 35.207062539941916 39.0 35.0 39.0 29.0 39.0 11 35.20913021671777 39.0 35.0 39.0 29.0 39.0 12 35.10178211734632 39.0 35.0 39.0 28.0 39.0 13 35.09363437067717 39.0 35.0 39.0 28.0 39.0 14 36.2073019892086 40.0 36.0 41.0 28.0 41.0 15 36.15244383863061 40.0 36.0 41.0 28.0 41.0 16 36.10877670546931 40.0 36.0 41.0 27.0 41.0 17 36.066424520899226 40.0 36.0 41.0 27.0 41.0 18 36.03849591884612 40.0 36.0 41.0 27.0 41.0 19 36.06968459030716 40.0 36.0 41.0 27.0 41.0 20 36.01070322043085 40.0 36.0 41.0 27.0 41.0 21 35.90761586189602 40.0 36.0 41.0 27.0 41.0 22 35.81043586238139 40.0 36.0 41.0 27.0 41.0 23 35.70123526699402 40.0 36.0 41.0 27.0 41.0 24 35.59144454241731 40.0 36.0 41.0 27.0 41.0 25 35.48351521230899 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8813325106213412 1101 2 3.5870446428862763 1101 3 2.0483256202627516 1101 4 1.1275308022634576 1101 5 0.5383216431886026 1101 6 0.6450401101285408 1101 7 0.5059074225624016 1101 8 0.5060658021350122 1101 9 0.6204158767524461 1101 10 0.5269559477485188 1101 11 0.4322412238420128 1101 12 0.45477236321546144 1101 13 0.40772428772818614 1101 14 0.46342389987316324 1101 15 0.6523563311063327 1101 16 0.456753088323552 1101 17 0.42860720955498266 1101 18 0.6414604200434155 1101 19 0.6489180028885144 1101 20 0.5555570384594759 1101 21 0.6598814452629611 1101 22 0.7035155849179375 1101 23 0.594180904533502 1101 24 0.6484692299577262 1101 25 0.6950814531811531 1102 1 1.2611500816624215 1102 2 4.025516165474155 1102 3 2.215712333531112 1102 4 1.3791249522482545 1102 5 1.0706069915975007 1102 6 0.9646724888372873 1102 7 0.7575938136430338 1102 8 0.8564984090150389 1102 9 0.9543245912589242 1102 10 0.969419695840017 1102 11 0.8385138499267129 1102 12 1.0126835616704426 1102 13 1.0386275248889234 1102 14 1.201613759064621 1102 15 1.0397735649849835 1102 16 1.075548620303806 1102 17 1.1771288433725005 1102 18 1.1567290230269336 1102 19 1.1639598625925132 1102 20 1.1663030875301956 1102 21 1.2671431737761907 1102 22 1.249683218250702 1102 23 1.457760569192729 1102 24 1.0831040632830806 1102 25 1.2307258826680467 1103 1 1.1679642178754008 1103 2 3.8399954278534985 1103 3 2.2848536562386315 1103 4 1.3625073359601885 1103 5 0.9921411691735145 1103 6 0.8086596857029207 1103 7 0.8393718633332696 1103 8 0.7392934011760772 1103 9 0.7708234859196352 1103 10 1.0318740190964206 1103 11 1.0024793342290792 1103 12 0.9107042613850851 1103 13 0.8643834465660092 1103 14 1.271252787700341 1103 15 1.0138625319097088 1103 16 0.9800167534782389 1103 17 1.1067870372875248 1103 18 1.2792204031999646 1103 19 1.2311520581996618 1103 20 1.2317993681923696 1103 21 1.159883566118566 1103 22 1.1351176059776478 1103 23 1.3901119852096926 1103 24 1.4836747003769943 1103 25 1.3858206022090442 1104 1 1.3887258105683884 1104 2 4.028077481089735 1104 3 2.3648621711413753 1104 4 1.6094125602185798 1104 5 1.3230079131611134 1104 6 1.1380787854495011 1104 7 0.9316463061074742 1104 8 1.1226071088771121 1104 9 1.2534722483970526 1104 10 1.2699547245705105 1104 11 1.250341100358746 1104 12 1.2190360531000763 1104 13 1.2560254011829812 1104 14 1.4143544794926157 1104 15 1.382336824546904 1104 16 1.4373504811704692 1104 17 1.3435748142295623 1104 18 1.334199682741712 1104 19 1.3276992762155118 1104 20 1.3156746680694411 1104 21 1.4390911410587606 1104 22 1.4048426588080787 1104 23 1.5443445306745076 1104 24 1.498290903875045 1104 25 1.5427470103343168 1105 1 1.0714650735947018 1105 2 3.697339646053159 1105 3 2.1318270238007386 1105 4 1.185651622500572 1105 5 0.8240313888568167 1105 6 0.5805422243563143 1105 7 0.5087440486592882 1105 8 0.507994737616869 1105 9 0.6107156302412236 1105 10 0.5507458422750204 1105 11 0.6236975952205412 1105 12 0.3699272653395056 1105 13 0.6039936123944756 1105 14 0.6879147084835111 1105 15 0.7906475682120657 1105 16 0.7494504162489051 1105 17 0.6371117725402371 1105 18 0.8137543720436042 1105 19 0.8308798635278976 1105 20 0.7478718502456232 1105 21 0.9050486060423992 1105 22 0.763543038656195 1105 23 0.7849067001938295 1105 24 0.7661989700950471 1105 25 0.7806156822611499 1106 1 0.9565306595899834 1106 2 3.768837091072168 1106 3 2.1404218148179623 1106 4 1.1124419429123051 1106 5 0.9643788479343343 1106 6 0.6872913777896485 1106 7 0.7141976800329886 1106 8 0.6096201761811884 1106 9 0.765015394302786 1106 10 0.8314407604300555 1106 11 0.7526148761828821 1106 12 0.7054984991874989 1106 13 0.4701596739340701 1106 14 0.713670047660294 1106 15 0.6960475633221535 1106 16 0.7848434737909855 1106 17 0.6808963839753019 1106 18 0.6661736578714113 1106 19 0.5371915223095769 1106 20 0.803587856162423 1106 21 0.6870561609622641 1106 22 0.5960351536670103 1106 23 0.657588574498547 1106 24 0.7640848455733362 1106 25 0.5229010760827393 1107 1 0.8714089169038353 1107 2 3.425045782198893 1107 3 1.9472553897780003 1107 4 1.0360960821636311 1107 5 0.7697035570529493 1107 6 0.8006555649945852 1107 7 0.5964912750666329 1107 8 0.6022301163321089 1107 9 0.47318720947694004 1107 10 0.4661718113203577 1107 11 0.571998170484207 1107 12 0.5017328399183256 1107 13 0.6456509196126632 1107 14 0.845429090075072 1107 15 0.7835067655822954 1107 16 0.7282091479294905 1107 17 0.6539854884695586 1107 18 0.4617738214341003 1107 19 0.6094252682669747 1107 20 0.7182642622439843 1107 21 0.526670301700527 1107 22 0.6472641042636482 1107 23 0.751170225738278 1107 24 0.7591742474543111 1107 25 0.7708851658935316 1108 1 0.9245593362164541 1108 2 3.6848331365705675 1108 3 2.05220376342675 1108 4 1.1853254841381968 1108 5 0.7760056883567756 1108 6 0.6509339756462538 1108 7 0.6449592893507301 1108 8 0.5462183529569273 1108 9 0.5767168683713777 1108 10 0.5421845442044884 1108 11 0.52543330225663 1108 12 0.5360130603200304 1108 13 0.5404158944862232 1108 14 0.6614340668876508 1108 15 0.7260329827199783 1108 16 0.7088794017792495 1108 17 0.7047499462716829 1108 18 0.7318664095382275 1108 19 0.6686635574068589 1108 20 0.610365672879496 1108 21 0.6398682269513856 1108 22 0.45821276876507255 1108 23 0.595729179961296 1108 24 0.6412205576648518 1108 25 0.6530859511957772 1109 1 0.684347469374007 1109 2 2.7392378196696185 1109 3 1.4276323274383103 1109 4 0.7952418388269535 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4 0.09914734270999759 2314 5 -0.1324606974792104 2314 6 -0.2854327628549953 2314 7 -0.41307654784757375 2314 8 -0.56583150241444 2314 9 -0.5974373649049696 2314 10 -0.6822242805199608 2314 11 -0.48291778114407435 2314 12 -0.6462532248974853 2314 13 -0.7083874881635097 2314 14 -1.4622782710569453 2314 15 -1.187043831895899 2314 16 -0.9738470085689315 2314 17 -0.706547690028394 2314 18 -0.7606681332192053 2314 19 -0.8322425094869104 2314 20 -0.713173627980602 2314 21 -0.695607212044699 2314 22 -0.689858251527987 2314 23 -0.6924856096200998 2314 24 -0.6190842846202713 2314 25 -0.5587676731135147 2315 1 -4.430850851680905 2315 2 -13.731496301671436 2315 3 -6.988027931045124 2315 4 -3.1222191467731903 2315 5 -2.0213904961130744 2315 6 -1.435606667865251 2315 7 -1.278386148491272 2315 8 -1.2027565001048828 2315 9 -1.2676959640561734 2315 10 -1.2431513627792583 2315 11 -1.3137220634803057 2315 12 -1.349249329493503 2315 13 -1.2073743321257808 2315 14 -1.4313033378023903 2315 15 -1.3506710304493765 2315 16 -1.454619103426566 2315 17 -1.2687846966343201 2315 18 -1.2627154079842455 2315 19 -1.2491400704292275 2315 20 -1.333154678684231 2315 21 -1.3769912711867818 2315 22 -1.3636515013956867 2315 23 -1.4557504217712491 2315 24 -1.5373616049157874 2315 25 -1.5498272078831832 2316 1 -1.551196368396866 2316 2 -1.5396282719091232 2316 3 -0.9883572116567017 2316 4 -1.0803442549775113 2316 5 -1.2829891510575493 2316 6 -1.4767776556731391 2316 7 -1.485926044062282 2316 8 -1.6600639836978388 2316 9 -1.616607567972821 2316 10 -1.7471526799017099 2316 11 -1.742782702032514 2316 12 -1.770547141256536 2316 13 -1.7202293696099389 2316 14 -1.9861472301804923 2316 15 -2.5234080506586665 2316 16 -2.15981863341365 2316 17 -2.1225455516194316 2316 18 -2.1160490225882 2316 19 -2.0906280696300286 2316 20 -2.245835910319805 2316 21 -2.30084064397753 2316 22 -2.2576238718911767 2316 23 -2.2659201491957646 2316 24 -2.1081242023239213 2316 25 -2.2041555506027137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77597.0 3 1.0 4 716.0 5 3618.0 6 4322.0 7 4003.0 8 3647.0 9 3607.0 10 3309.0 11 3337.0 12 3245.0 13 3264.0 14 3288.0 15 3516.0 16 3536.0 17 3821.0 18 4458.0 19 5439.0 20 6955.0 21 9182.0 22 11150.0 23 13741.0 24 16023.0 25 18248.0 26 20699.0 27 23918.0 28 28037.0 29 33219.0 30 42176.0 31 54147.0 32 70004.0 33 93083.0 34 127623.0 35 183360.0 36 284949.0 37 386291.0 38 914811.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30482053439252 19.60652661041766 13.017748367942922 25.0709044872469 2 15.644573370864274 21.72111064080929 40.68192527393892 21.952390714387512 3 18.05042995704474 25.796249706755543 30.389266848410816 25.764053487788896 4 11.74247878528034 17.648422142585567 39.844964689322666 30.764134382811427 5 12.385391605981722 38.503930701863666 36.555689122658286 12.55498856949633 6 29.927347416226258 38.48778917241095 19.034394498433453 12.550468912929343 7 26.604431429334152 32.247668200975596 23.602862065897085 17.545038303793167 8 23.92632081348035 37.86295574233318 21.546834173293316 16.66388927089316 9 26.127110348900235 15.218780588430395 21.277332405737077 37.3767766569323 10 14.458812299279225 29.450722797026295 35.665078427724346 20.425386475970132 11 32.935639920075715 22.638916977438377 26.178114660604933 18.247328441880974 12 22.811506508004562 25.20887094817056 32.94498329517785 19.034639248647032 13 28.429122555249325 22.813506891048068 26.43882025839509 22.318550295307517 14 20.13072635640729 23.17088264559082 28.807566920407385 27.890824077594505 15 21.96356488185282 32.38167080579532 24.083338052209648 21.57142626014221 16 20.911282428792155 28.760526464806617 29.289579912148007 21.038611194253217 17 20.666831424262 29.40943300601581 28.876775205788576 21.046960363933618 18 20.423040520316786 27.896122701570174 31.495465833987236 20.185370944125808 19 22.712367740998662 27.290928748502928 28.691714324197402 21.304989186301004 20 22.796374098368215 27.9169024435723 29.381476449476867 19.905247008582617 21 22.12858540042179 27.357039021679252 28.151677899040493 22.362697678858463 22 22.37171340299761 28.991383515664754 28.392573854135406 20.244329227202236 23 21.172812216487195 28.66282872317663 28.991627739165992 21.172731321170183 24 22.27231966437635 27.41268935356772 29.757516605030226 20.5574743770257 25 21.82574849645455 28.101263467597075 29.228907387910073 20.8440806480383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 305.0 1 305.0 2 530.5 3 756.0 4 756.0 5 756.0 6 2077.0 7 3398.0 8 3398.0 9 3398.0 10 5004.5 11 6611.0 12 6611.0 13 6611.0 14 10571.0 15 14531.0 16 14531.0 17 14531.0 18 25652.5 19 36774.0 20 36774.0 21 36774.0 22 57527.0 23 78280.0 24 78280.0 25 78280.0 26 110536.5 27 142793.0 28 142793.0 29 142793.0 30 182010.0 31 221227.0 32 221227.0 33 221227.0 34 245751.0 35 270275.0 36 270275.0 37 270275.0 38 283316.5 39 296358.0 40 296358.0 41 296358.0 42 306386.0 43 316414.0 44 316414.0 45 316414.0 46 315098.0 47 313782.0 48 313782.0 49 313782.0 50 296281.0 51 278780.0 52 278780.0 53 278780.0 54 240571.5 55 202363.0 56 202363.0 57 202363.0 58 169734.0 59 137105.0 60 137105.0 61 137105.0 62 109263.0 63 81421.0 64 81421.0 65 81421.0 66 61639.5 67 41858.0 68 41858.0 69 41858.0 70 30312.5 71 18767.0 72 18767.0 73 18767.0 74 12917.5 75 7068.0 76 7068.0 77 7068.0 78 4732.0 79 2396.0 80 2396.0 81 2396.0 82 1584.5 83 773.0 84 773.0 85 773.0 86 493.5 87 214.0 88 214.0 89 214.0 90 138.5 91 63.0 92 63.0 93 63.0 94 42.0 95 21.0 96 21.0 97 21.0 98 14.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.4919226320004535 3 0.0 4 0.0 5 3.235800901170551E-4 6 0.001051635292880429 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 8.089502252926378E-5 14 0.0 15 0.0 16 0.0 17 8.493977365572697E-4 18 0.0 19 5.258176464402145E-4 20 6.876076914987421E-4 21 8.898452478219015E-4 22 0.004125646148992453 23 3.64027601381687E-4 24 0.0019010330294376985 25 6.876076914987421E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2472340.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.18623183792145 #Duplication Level Percentage of deduplicated Percentage of total 1 85.79640788090363 63.64912205913588 2 10.177908797196816 15.101214033081265 3 2.220667112855359 4.942287758074062 4 0.7289927198672216 2.163248916969065 5 0.346553477757522 1.285474832257874 6 0.16891794467988588 0.7518831483364322 7 0.11303926196028118 0.5870169826201068 8 0.0777321423937398 0.4613323789504242 9 0.055430320071133396 0.37009499180825645 >10 0.2772424827983817 3.6833586588025886 >50 0.018062930535196413 0.9440519959215269 >100 0.01579361060482075 2.3879231224987714 >500 0.001763426915839652 0.9632587240786515 >1k 0.001377677277990354 2.2283892085486885 >5k 1.1021418223922833E-4 0.4813431889163473 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6766 0.27366786121649933 No Hit GTCCTACAGTGGACATTTCTAAATT 5008 0.20256113641327647 No Hit CTGTAGGACGTGGAATATGGCAAGA 4927 0.19928488800084132 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4900 0.19819280519669624 No Hit GGTATCAACGCAGAGTACTTTTTTT 4493 0.18173066811199107 No Hit CTTTAGGACGTGAAATATGGCGAGG 4438 0.17950605499243633 No Hit TATCAACGCAGAGTACTTTTTTTTT 4405 0.17817128712070346 No Hit GTCCTACAGTGTGCATTTCTCATTT 2652 0.10726679987380376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.044751126463189E-5 0.0 6 0.0 0.0 0.0 4.044751126463189E-5 0.0 7 0.0 0.0 0.0 8.089502252926378E-5 0.0 8 0.0 0.0 0.0 8.089502252926378E-5 0.0 9 8.089502252926378E-5 0.0 0.0 2.4268506758779132E-4 0.0 10 1.2134253379389566E-4 0.0 0.0 2.4268506758779132E-4 0.0 11 1.6179004505852755E-4 0.0 0.0 2.831325788524232E-4 0.0 12 1.6179004505852755E-4 0.0 0.0 2.831325788524232E-4 0.0 13 1.6179004505852755E-4 0.0 0.0 3.235800901170551E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1460 0.0 14.169859 1 TGAGCCG 70 1.1521386E-4 12.143611 5 TAGGACC 935 0.0 12.121964 4 AAAGCGT 60 0.0061366735 11.019202 6 TTAATCC 350 0.0 10.794321 4 CCCTACA 250 0.0 10.793736 2 CGTATGT 65 0.0073091513 10.762985 1 GGCGAGG 1275 0.0 10.667544 19 GGACCTG 1730 0.0 10.5369425 6 TCCAACG 145 1.5805199E-8 10.422314 18 CTAATAC 200 1.8189894E-12 10.389534 3 AGGACCT 1855 0.0 10.386988 5 AAGACTA 210 0.0 10.344558 5 CTGTAGG 2935 0.0 10.215537 1 AGGACGT 3200 0.0 10.21244 5 GACGTGG 1605 0.0 10.180626 7 TGTAGGA 2950 0.0 10.163594 2 GTCCTAA 1730 0.0 10.051971 1 GTAGGAC 3190 0.0 10.037196 3 GGACGTG 3235 0.0 10.014361 6 >>END_MODULE