FastQCFastQC Report
Thu 26 May 2016
SRR1511846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472340
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84430.34149833760728704No Hit
GGTATCAACGCAGAGTACTTTTTTT58270.23568764813901No Hit
TATCAACGCAGAGTACTTTTTTTTT57430.2322900571927809No Hit
GTCCTACAGTGGACATTTCTAAATT55770.22557577032285203No Hit
GTCCTAAAGTGTGTATTTCTCATTT53440.21615150019819282No Hit
CTGTAGGACGTGGAATATGGCAAGA51970.21020571604229193No Hit
CTTTAGGACGTGAAATATGGCGAGG50260.20328919161603987No Hit
GTCCTACAGTGTGCATTTCTCATTT29830.12065492610239692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28080.11357661163108633No Hit
CTGAAGGACCTGGAATATGGCGAGA26180.1058915844908063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.014.1813731
TAGGACC9300.011.4403614
TGGACCG600.005879233611.08284955
TTGGCGT700.001492485610.8566684
GGCGAGG12300.010.81034919
TCCAACG2200.010.792818
TGGACTG3300.010.3631845
GTATCAA33450.010.3493721
GCGCCAC1201.5298101E-610.28892813
TGTAGGA31500.010.2233632
AGGACCT19100.010.0964185
GTGGTAT4350.010.0849111
CTGTAGG32600.09.9756291
TCTATAC2900.09.8271583
GGACGTG31900.09.6761086
GTAGGAC31450.09.6657143
TGGCGAG30750.09.63665518
AGGACGT32250.09.6026825
CTAATAC2500.09.4995853
GTGCGCC1106.859016E-59.49747311
Produced by FastQC (version 0.11.5)