FastQCFastQC Report
Thu 26 May 2016
SRR1511845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807094
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57650.31902048260909505No Hit
GGTATCAACGCAGAGTACTTTTTTT38770.21454334970953368No Hit
TATCAACGCAGAGTACTTTTTTTTT36400.20142837063262894No Hit
GTCCTAAAGTGTGTATTTCTCATTT30180.1670084677388116No Hit
GTCCTACAGTGGACATTTCTAAATT29640.16402024465799786No Hit
CTGTAGGACGTGGAATATGGCAAGA28830.15953791003677728No Hit
CTTTAGGACGTGAAATATGGCGAGG27000.1494111540406863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.013.5061931
TGTCGAG1155.804395E-912.31966218
GGCGAGG8900.011.99206219
TAGGACC5700.011.7649174
CCCCGGT600.006135379411.01925519
GTCGAGG1053.715928E-610.79437119
CGTTAGG650.007305717610.7633971
TTAGGAC13150.010.55837253
CCGTGCA901.0094936E-410.4942379
GTGGTAT3450.010.4291311
GTCCTAA8750.010.3943671
GTATCAA22600.010.3925971
AGGACGT19350.010.1528455
TATGTCG1401.05450454E-710.11972316
GACGTGA11400.010.1076077
CGGTAGG800.0029684119.9945821
GGACGTG19450.09.9549646
GGACGGG951.743193E-49.94218356
GCCGGTT1054.3751726E-59.8948411
ATAAGGG1151.0996337E-59.8557293
Produced by FastQC (version 0.11.5)