##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511845_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1807094 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.418792270905666 31.0 30.0 34.0 25.0 34.0 2 26.66397210106392 31.0 26.0 34.0 10.0 34.0 3 28.409230510421704 31.0 28.0 34.0 16.0 34.0 4 32.79759160287179 35.0 33.0 37.0 26.0 37.0 5 33.158969594276776 35.0 35.0 37.0 27.0 37.0 6 33.36522062493705 37.0 35.0 37.0 28.0 37.0 7 33.35185054014899 37.0 35.0 37.0 27.0 37.0 8 33.368322843194655 37.0 35.0 37.0 27.0 37.0 9 34.915868239283625 39.0 35.0 39.0 28.0 39.0 10 34.785534676115354 39.0 35.0 39.0 27.0 39.0 11 34.78770777834468 39.0 35.0 39.0 27.0 39.0 12 34.66995961471844 39.0 35.0 39.0 27.0 39.0 13 34.65362344183534 39.0 35.0 39.0 27.0 39.0 14 35.73446373016567 40.0 36.0 41.0 27.0 41.0 15 35.67363014873604 40.0 36.0 41.0 27.0 41.0 16 35.62279383363566 40.0 36.0 41.0 27.0 41.0 17 35.57940151425438 40.0 36.0 41.0 27.0 41.0 18 35.54633073874408 40.0 36.0 41.0 27.0 41.0 19 35.57592632148632 40.0 36.0 41.0 27.0 41.0 20 35.511997715669466 40.0 36.0 41.0 26.0 41.0 21 35.40507854046331 40.0 36.0 41.0 26.0 41.0 22 35.30396537202824 40.0 36.0 41.0 25.0 41.0 23 35.18829402344316 40.0 36.0 41.0 25.0 41.0 24 35.06861236880871 40.0 35.0 41.0 25.0 41.0 25 34.956765945767074 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1692701159927168 1101 2 3.7510600066308264 1101 3 2.220353466000887 1101 4 1.3564610886550668 1101 5 0.8245429646725171 1101 6 0.7926127324775933 1101 7 0.6844119686418537 1101 8 0.5629175659307393 1101 9 0.7619015101176743 1101 10 0.7245587911298941 1101 11 0.6722328981949133 1101 12 0.6465023261344527 1101 13 0.5969880718023717 1101 14 0.510227450884301 1101 15 0.6557044158379668 1101 16 0.6043811199336204 1101 17 0.7784859135203988 1101 18 0.758865471038483 1101 19 0.7454654239172456 1101 20 0.8112774595995234 1101 21 0.8620372468041992 1101 22 0.7218274838636916 1101 23 0.4937016199476716 1101 24 0.6689815523286526 1101 25 0.7591149790815948 1102 1 1.0395356134960672 1102 2 3.846087441811715 1102 3 2.17000735811002 1102 4 1.2883767456346575 1102 5 0.9046118900099884 1102 6 0.8300641407729898 1102 7 0.8026054269887624 1102 8 0.7073324411640272 1102 9 0.7474926913610318 1102 10 0.8243046824358871 1102 11 0.6865972808457457 1102 12 0.8024409648096622 1102 13 0.7584367875989102 1102 14 0.9273565822078069 1102 15 0.9514587927943907 1102 16 0.9135321599030775 1102 17 0.8899983210686102 1102 18 0.8827648545286593 1102 19 1.0112550535176155 1102 20 0.9459188602255821 1102 21 0.8774055562901566 1102 22 0.924136021849975 1102 23 0.9669881143339154 1102 24 0.8099740245231715 1102 25 0.7896417608871502 1103 1 1.1504264518976584 1103 2 3.7359455306224945 1103 3 2.1966877412178434 1103 4 1.4569214852048447 1103 5 1.0701037688925368 1103 6 0.9330049203558133 1103 7 0.8994777926462234 1103 8 0.6704468829572576 1103 9 0.86559378978761 1103 10 0.9671501454530969 1103 11 1.0325405322455694 1103 12 0.8672110636029657 1103 13 1.1674927344858048 1103 14 1.2398505296693898 1103 15 0.9652581834922955 1103 16 0.9160631131798596 1103 17 1.0750785955879394 1103 18 1.2076166963260206 1103 19 1.1970243251968142 1103 20 1.1302105187560514 1103 21 1.3886700248336226 1103 22 1.1798908944410655 1103 23 1.353201582799592 1103 24 1.288857214103757 1103 25 1.2550430699817525 1104 1 1.0247915360302677 1104 2 3.6659195666516773 1104 3 2.113486230101394 1104 4 1.336863149702289 1104 5 1.0908115373927032 1104 6 0.9132149014668585 1104 7 0.7293670619840427 1104 8 0.7722236462045586 1104 9 0.832735016435052 1104 10 0.9013992260348473 1104 11 0.8679262874366955 1104 12 0.9001799669088655 1104 13 0.9506735545201082 1104 14 1.112783924408518 1104 15 1.0581644795883562 1104 16 0.9682680483366823 1104 17 0.9665772306439706 1104 18 0.9072188935854513 1104 19 1.0557042341560532 1104 20 0.9904589452326249 1104 21 1.1346970033349209 1104 22 1.074830256221297 1104 23 1.1141561758861016 1104 24 1.0872292931570442 1104 25 1.156920618822717 1105 1 1.1975252241401044 1105 2 3.929030102913309 1105 3 2.3157613899545915 1105 4 1.4264420724601052 1105 5 0.9813145092358013 1105 6 0.9054887528940085 1105 7 0.7212751976056211 1105 8 0.8917140464327815 1105 9 0.9084221009829676 1105 10 0.7041100274136269 1105 11 0.7860874554965136 1105 12 0.5955687303175523 1105 13 0.7765917585873865 1105 14 0.9744779285997751 1105 15 1.0644717366340188 1105 16 0.9402237591873899 1105 17 0.904304730542087 1105 18 1.062641676071415 1105 19 1.0785780954500197 1105 20 1.132807119549291 1105 21 1.2938874093545394 1105 22 1.2068086178573907 1105 23 1.0508380074251775 1105 24 0.9542556697392186 1105 25 0.996340278245853 1106 1 1.0678614496322822 1106 2 3.8329650168316576 1106 3 2.181270423380866 1106 4 1.2874910621287654 1106 5 0.9344611511180361 1106 6 0.7570869448617756 1106 7 0.7177788219443926 1106 8 0.5828445668866777 1106 9 0.8000021279610579 1106 10 0.8457165129307072 1106 11 0.9188223447843598 1106 12 0.7909324245838434 1106 13 0.7080173831532264 1106 14 0.8579052619061684 1106 15 0.9210137306649671 1106 16 0.9559253145646309 1106 17 0.8515023383053091 1106 18 0.8846390167477196 1106 19 1.037793075408885 1106 20 1.0521507168714948 1106 21 1.0328434227550716 1106 22 1.0329792879415365 1106 23 0.8758379593187371 1106 24 0.9668279977635237 1106 25 0.9958830746921308 1107 1 1.0609698514424544 1107 2 3.4733045726253913 1107 3 1.9682129326103528 1107 4 1.3204540995230758 1107 5 1.044197887452441 1107 6 0.7670268787667354 1107 7 0.69891978002466 1107 8 0.6587790524172235 1107 9 0.6669270713932391 1107 10 0.7000476728687772 1107 11 0.5922019744139888 1107 12 0.6652822667893972 1107 13 0.6844885186778171 1107 14 1.0062837750656257 1107 15 0.9582167496003038 1107 16 0.8956500674525714 1107 17 0.8763181207276034 1107 18 0.8014281229761337 1107 19 0.8601023823041984 1107 20 0.9206971471442102 1107 21 0.8718908504078016 1107 22 0.8973735656597697 1107 23 0.9911723049183578 1107 24 0.9891856850327869 1107 25 0.9537582830998943 1108 1 0.9393129757650094 1108 2 3.504295098629033 1108 3 1.8863105408480756 1108 4 1.048986460915387 1108 5 0.6070688841393519 1108 6 0.5097037684532211 1108 7 0.3484442268209875 1108 8 0.35724502425598814 1108 9 0.3612059893227624 1108 10 0.3699846066274972 1108 11 0.43246121833488615 1108 12 0.36175489036548214 1108 13 0.37953850896352037 1108 14 0.48550188916260595 1108 15 0.4789446316789565 1108 16 0.3875750577382746 1108 17 0.22570784555735202 1108 18 0.3731730025188469 1108 19 0.47785692246004174 1108 20 0.4796287405957571 1108 21 0.5965077747589902 1108 22 0.4988298155319484 1108 23 0.5323852116406513 1108 24 0.6192102775435728 1108 25 0.4569713508750155 1109 1 0.7308176707944547 1109 2 2.8100589099362594 1109 3 1.6183754568453281 1109 4 1.1566397269340811 1109 5 0.6998323315970723 1109 6 0.7102936158897251 1109 7 0.6200577369913418 1109 8 0.37671112203405954 1109 9 0.37479506823519415 1109 10 0.529025581565385 1109 11 0.5138574230358444 1109 12 0.5866275951857887 1109 13 0.4839406708434737 1109 14 0.6084788516181661 1109 15 0.5279329005866913 1109 16 0.5373091138438042 1109 17 0.6298737287506313 1109 18 0.6286880343310912 1109 19 0.629588425957138 1109 20 0.5559167484913772 1109 21 0.34313680291724324 1109 22 0.4878693332838395 1109 23 0.3962274207465484 1109 24 0.405741170631714 1109 25 0.4078695466165172 1110 1 0.7716057648399932 1110 2 3.5187419894290883 1110 3 1.9007676464150656 1110 4 1.1159979586570543 1110 5 0.6881262701162854 1110 6 0.5732688038313398 1110 7 0.3505242452192405 1110 8 0.3036323822269509 1110 9 0.38891193945368485 1110 10 0.40388595882915723 1110 11 0.37780754563002716 1110 12 0.386468205635218 1110 13 0.3353050659333192 1110 14 0.5181453657789632 1110 15 0.6456213185455724 1110 16 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9 0.8624420659887875 1203 10 0.8550127369501297 1203 11 0.703098699757561 1203 12 0.6897425799166967 1203 13 0.6221949769973634 1203 14 0.7138453885701352 1203 15 0.6661261368016795 1203 16 0.640356600221196 1203 17 0.7728680264254422 1203 18 0.6732388761802 1203 19 0.7074590264707012 1203 20 0.7861073477706881 1203 21 0.7437826014500857 1203 22 0.9863491739363042 1203 23 0.8163644938315144 1203 24 1.1078754364280172 1203 25 0.9227829864065384 1204 1 -0.4579371816890969 1204 2 0.7272934582597088 1204 3 0.16737040510921375 1204 4 0.025468785782273073 1204 5 0.16560056759303876 1204 6 0.25475815683742553 1204 7 0.5610815078772973 1204 8 0.5083964367171632 1204 9 0.6433944768479023 1204 10 0.7879679195653893 1204 11 0.5212828017263647 1204 12 0.6828742491496129 1204 13 0.5562959862948205 1204 14 0.6178820550239692 1204 15 0.48702582009434536 1204 16 0.6791538590297392 1204 17 0.5820610176227419 1204 18 0.7468052059026462 1204 19 0.561595010697097 1204 20 0.5121333493833617 1204 21 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-1.4374311347053137 2315 15 -1.5084185069056417 2315 16 -1.6642350107514758 2315 17 -1.470942197976676 2315 18 -1.4805571132116881 2315 19 -1.5206836180938694 2315 20 -1.5345244551679045 2315 21 -1.8379865566678788 2315 22 -1.7183531804549048 2315 23 -1.6268791040813255 2315 24 -1.8452682550245996 2315 25 -1.857808482669455 2316 1 -1.6057384485841908 2316 2 -1.1928541670495356 2316 3 -0.7830943548929774 2316 4 -1.137883397146254 2316 5 -1.2540260334108702 2316 6 -1.3879366504099195 2316 7 -1.4679009300739274 2316 8 -1.7149844367914113 2316 9 -1.687680908889206 2316 10 -1.8553996359508318 2316 11 -1.807436648021394 2316 12 -1.685557894414977 2316 13 -1.758727741647256 2316 14 -1.9483483525656027 2316 15 -2.4182570713429428 2316 16 -2.1598638547430085 2316 17 -2.091045488385106 2316 18 -2.0306270662058594 2316 19 -2.0200185209731742 2316 20 -2.108775108384698 2316 21 -2.0442512359231344 2316 22 -2.2469193333543487 2316 23 -2.1750280681165464 2316 24 -1.9302454367364632 2316 25 -2.2553452673930394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68228.0 3 4.0 4 619.0 5 3077.0 6 3673.0 7 3369.0 8 3021.0 9 3050.0 10 2858.0 11 2821.0 12 2723.0 13 2756.0 14 2812.0 15 2854.0 16 3078.0 17 3251.0 18 3707.0 19 4514.0 20 5818.0 21 7585.0 22 9375.0 23 11252.0 24 13043.0 25 14984.0 26 16818.0 27 18922.0 28 22185.0 29 26184.0 30 32610.0 31 41235.0 32 52713.0 33 69132.0 34 93987.0 35 132626.0 36 205121.0 37 273811.0 38 643278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62733427259457 19.44613838571762 13.138331486906601 24.78819585478121 2 16.066650036948424 21.557911822675674 39.90968423444155 22.46575390593435 3 18.177416338054357 25.32734877101025 30.214864307003396 26.280370583931994 4 12.152162532773614 17.494053989443824 39.04987787021594 31.303905607566623 5 12.972390879449987 37.96425622715934 36.062629075350124 13.000723818040555 6 30.72911075227522 37.7265397509128 18.665104660673197 12.879244836138785 7 27.205668327159515 32.065293781065066 22.993103845179057 17.73593404659636 8 24.45766517956454 37.032882628131134 21.31023621350079 17.199215978803537 9 26.189008430109332 15.3609054094585 21.10244403445532 37.34764212597684 10 15.046754623721842 29.139048660445997 34.88352017105917 20.93067654477299 11 33.23341231834094 22.746022066367328 25.491645702990546 18.528919912301188 12 22.97107953432417 25.148110723625887 32.232578936126174 19.648230805923763 13 28.544423858884883 22.52430977504189 26.17033333111983 22.760933034953396 14 20.642257680010005 22.921220478846145 28.36011851071389 28.07640333042996 15 22.584602682538925 31.40794004075051 23.988458818412322 22.01899845829824 16 21.50839967373031 28.57936554490248 28.472951600746832 21.43928318062038 17 21.218825466884773 28.990406644074103 28.32253508968039 21.468232799360738 18 20.895813942163496 27.714440975400283 30.616946323766225 20.772798758669996 19 22.88965611506253 27.285791995626106 28.49171069240164 21.332841196909722 20 23.109040249905235 27.517023272286583 28.96853219411372 20.40540428369446 21 22.58763022375299 26.978797863074284 28.028722548285025 22.404849364887703 22 22.767049526428945 28.352448651505384 28.116977446230386 20.763524375835285 23 21.764451003796715 28.03990284927859 28.62902712594676 21.566619020977935 24 22.51631514783934 27.107355969327024 29.25326897844735 21.123059904386288 25 22.268060519833963 27.686977367317496 28.72201903958713 21.322943073261406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 346.0 1 346.0 2 503.5 3 661.0 4 661.0 5 661.0 6 1732.5 7 2804.0 8 2804.0 9 2804.0 10 3874.5 11 4945.0 12 4945.0 13 4945.0 14 7627.0 15 10309.0 16 10309.0 17 10309.0 18 17818.5 19 25328.0 20 25328.0 21 25328.0 22 39009.0 23 52690.0 24 52690.0 25 52690.0 26 74554.5 27 96419.0 28 96419.0 29 96419.0 30 122025.5 31 147632.0 32 147632.0 33 147632.0 34 166098.0 35 184564.0 36 184564.0 37 184564.0 38 196251.5 39 207939.0 40 207939.0 41 207939.0 42 216532.0 43 225125.0 44 225125.0 45 225125.0 46 229037.5 47 232950.0 48 232950.0 49 232950.0 50 222587.5 51 212225.0 52 212225.0 53 212225.0 54 186798.5 55 161372.0 56 161372.0 57 161372.0 58 136456.0 59 111540.0 60 111540.0 61 111540.0 62 89977.0 63 68414.0 64 68414.0 65 68414.0 66 52230.5 67 36047.0 68 36047.0 69 36047.0 70 26280.0 71 16513.0 72 16513.0 73 16513.0 74 11393.0 75 6273.0 76 6273.0 77 6273.0 78 4185.0 79 2097.0 80 2097.0 81 2097.0 82 1346.0 83 595.0 84 595.0 85 595.0 86 405.0 87 215.0 88 215.0 89 215.0 90 141.5 91 68.0 92 68.0 93 68.0 94 44.0 95 20.0 96 20.0 97 20.0 98 11.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.495452920545361 3 0.0 4 0.0 5 5.533746445951345E-4 6 8.853994313522153E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.1067492891902691E-4 14 0.0 15 0.0 16 0.0 17 5.533746445951345E-4 18 0.0 19 3.873622512165941E-4 20 4.98037180135621E-4 21 6.640495735141614E-4 22 0.004371659692301562 23 2.7668732229756723E-4 24 0.001715461398244917 25 9.407368958117286E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1807094.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.07072875466065 #Duplication Level Percentage of deduplicated Percentage of total 1 85.14924501376177 63.92215876092251 2 10.769767655930892 16.169886128982107 3 2.343634549108823 5.278150606085997 4 0.760938134829898 2.284967212755707 5 0.3280145514058366 1.2312145708084623 6 0.16886450074432835 0.76060686790012 7 0.0982597090713218 0.5163499577043548 8 0.06422036153081616 0.3856855472804914 9 0.04358897035853766 0.2945030193432651 >10 0.2376479937059376 3.1327721608664016 >50 0.018113796256739305 0.9271016133936353 >100 0.01479200137868914 2.1839541986176596 >500 0.0017177826686183431 0.8633270086722359 >1k 0.0011202930447510927 1.7260933425758826 >5k 7.468620298340618E-5 0.32322900409114413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5765 0.31902048260909505 No Hit GGTATCAACGCAGAGTACTTTTTTT 3877 0.21454334970953368 No Hit TATCAACGCAGAGTACTTTTTTTTT 3640 0.20142837063262894 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3018 0.1670084677388116 No Hit GTCCTACAGTGGACATTTCTAAATT 2964 0.16402024465799786 No Hit CTGTAGGACGTGGAATATGGCAAGA 2883 0.15953791003677728 No Hit CTTTAGGACGTGAAATATGGCGAGG 2700 0.1494111540406863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 5.533746445951345E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1110 0.0 13.506193 1 TGTCGAG 115 5.804395E-9 12.319662 18 GGCGAGG 890 0.0 11.992062 19 TAGGACC 570 0.0 11.764917 4 CCCCGGT 60 0.0061353794 11.019255 19 GTCGAGG 105 3.715928E-6 10.794371 19 CGTTAGG 65 0.0073057176 10.763397 1 TTAGGAC 1315 0.0 10.5583725 3 CCGTGCA 90 1.0094936E-4 10.494237 9 GTGGTAT 345 0.0 10.429131 1 GTCCTAA 875 0.0 10.394367 1 GTATCAA 2260 0.0 10.392597 1 AGGACGT 1935 0.0 10.152845 5 TATGTCG 140 1.05450454E-7 10.119723 16 GACGTGA 1140 0.0 10.107607 7 CGGTAGG 80 0.002968411 9.994582 1 GGACGTG 1945 0.0 9.954964 6 GGACGGG 95 1.743193E-4 9.9421835 6 GCCGGTT 105 4.3751726E-5 9.89484 11 ATAAGGG 115 1.0996337E-5 9.855729 3 >>END_MODULE