FastQCFastQC Report
Thu 26 May 2016
SRR1511845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807094
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75160.4159163828777031No Hit
GGTATCAACGCAGAGTACTTTTTTT51200.28332781803270884No Hit
TATCAACGCAGAGTACTTTTTTTTT49840.275801922866215No Hit
GTCCTACAGTGGACATTTCTAAATT33170.18355436961220611No Hit
CTGTAGGACGTGGAATATGGCAAGA32880.18194958314288023No Hit
GTCCTAAAGTGTGTATTTCTCATTT32570.1802341217446353No Hit
CTTTAGGACGTGAAATATGGCGAGG32330.178906022597607No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23480.1299323665509376No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18720.10359173346820919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAT651.8215724E-716.1468771
GGTATCA14350.013.8299511
CGTGCAT500.001503304913.29577710
TAGGACC5100.012.8527724
GTCCTAT1950.012.2324831
AGGACCT11650.010.4376315
AATGTCC5800.010.315698
GGCGAGG8800.010.25327719
GCACTCG856.6133245E-410.05563113
GTATTAC1356.07075E-79.8947191
TGTCCAC6950.09.83860210
GGACCTG11200.09.8361626
GTGGTAT4600.09.7487571
ATAAACC2251.8189894E-129.7109843
GTCCTAC15850.09.6918311
ATTTAGA6700.09.6837451
TGGCGAG18000.09.65606918
GTCCTAA11500.09.6243041
GTATTAA2600.09.5413361
TTTAGAA7000.09.4998752
Produced by FastQC (version 0.11.5)