##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511845_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1807094 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.574367465112495 33.0 31.0 34.0 30.0 34.0 2 31.76254251300707 34.0 31.0 34.0 30.0 34.0 3 31.876205111632267 34.0 31.0 34.0 29.0 34.0 4 35.42294866786122 37.0 35.0 37.0 33.0 37.0 5 35.152411551363684 37.0 35.0 37.0 32.0 37.0 6 35.12406770206752 37.0 35.0 37.0 32.0 37.0 7 35.00721600536552 37.0 35.0 37.0 32.0 37.0 8 34.944687437399494 37.0 35.0 37.0 32.0 37.0 9 36.554460919022475 39.0 37.0 39.0 32.0 39.0 10 36.41561590044569 39.0 37.0 39.0 32.0 39.0 11 36.40007160667901 39.0 37.0 39.0 32.0 39.0 12 36.27943704090656 39.0 37.0 39.0 32.0 39.0 13 36.271685922259714 39.0 37.0 39.0 32.0 39.0 14 37.44733644182317 40.0 38.0 41.0 32.0 41.0 15 37.40450469095686 40.0 37.0 41.0 32.0 41.0 16 37.330942939326896 40.0 37.0 41.0 32.0 41.0 17 37.288716580321775 40.0 37.0 41.0 32.0 41.0 18 37.239680392940265 40.0 37.0 41.0 32.0 41.0 19 37.26971922877283 40.0 37.0 41.0 32.0 41.0 20 37.16617121190154 40.0 37.0 41.0 31.0 41.0 21 37.077498458851615 40.0 37.0 41.0 31.0 41.0 22 37.16232802499483 40.0 37.0 41.0 32.0 41.0 23 37.07729647710634 40.0 37.0 41.0 31.0 41.0 24 36.99964030648101 40.0 37.0 41.0 31.0 41.0 25 36.619034206300284 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29214515066442104 1101 2 0.2892527634620201 1101 3 0.3750328726532608 1101 4 0.3175372632358133 1101 5 0.3641684600288002 1101 6 0.39655339800359 1101 7 0.3062955760206023 1101 8 0.37208747012174115 1101 9 0.357384909472799 1101 10 0.427205295864411 1101 11 0.37160800976405284 1101 12 0.26640791287953647 1101 13 0.4711633173068961 1101 14 0.40481833723023186 1101 15 0.266836612405676 1101 16 0.31733279397656133 1101 17 0.33581008004092183 1101 18 0.21917747735752613 1101 19 0.3102981142146959 1101 20 0.3364631786249106 1101 21 0.4085380299877741 1101 22 0.3361598788419897 1101 23 0.1981747009053194 1101 24 0.1422041893506858 1101 25 0.234423041529503 1102 1 0.29467636291430566 1102 2 0.22211885937159437 1102 3 0.3411462275134163 1102 4 0.2978775779751004 1102 5 0.18154840848291087 1102 6 0.34941738731259164 1102 7 0.36985648804699167 1102 8 0.33649003490793206 1102 9 0.24184527411219392 1102 10 0.3664167031065091 1102 11 0.39058881007477453 1102 12 0.3763099230373044 1102 13 0.48517714845628035 1102 14 0.5069333312889626 1102 15 0.6412280846232363 1102 16 0.5639828053324507 1102 17 0.748213349221686 1102 18 0.715118979692889 1102 19 0.659021975086894 1102 20 0.6486004894834494 1102 21 0.7776588863209071 1102 22 0.5345687370973948 1102 23 0.6102504595283875 1102 24 0.5589761860459603 1102 25 0.6613544486399405 1103 1 0.27815710036352215 1103 2 0.279516371044064 1103 3 0.32097162864788586 1103 4 0.29509023520218847 1103 5 0.3982955639883059 1103 6 0.3719673594510695 1103 7 0.35380198106891214 1103 8 0.18590248226077222 1103 9 0.42795428171815075 1103 10 0.3833082463807713 1103 11 0.4216452776784152 1103 12 0.5483163176929722 1103 13 0.49905313849281185 1103 14 0.5991291684125741 1103 15 0.48938477908792066 1103 16 0.2650270964194661 1103 17 0.29986511672165506 1103 18 0.4224793426705631 1103 19 0.5867745108028259 1103 20 0.5356200369996458 1103 21 0.23879166750398184 1103 22 0.4029353534386928 1103 23 0.5532168939363373 1103 24 0.4529670671526702 1103 25 0.5076124320373339 1104 1 0.1544544645866388 1104 2 0.10528077303841954 1104 3 0.16297519285364714 1104 4 0.037386144375012975 1104 5 0.11780458463266541 1104 6 0.09688275962326998 1104 7 0.07744921814198591 1104 8 0.15339412239612926 1104 9 0.060821064521860535 1104 10 0.21580726188573607 1104 11 0.13996622973517248 1104 12 0.34265697299956344 1104 13 0.3582670108622068 1104 14 0.3178973148253377 1104 15 0.36096991621639773 1104 16 0.43090950432745956 1104 17 0.46423574072464646 1104 18 0.47295890855831146 1104 19 0.4284877324043137 1104 20 0.5110109983340223 1104 21 0.422671979605596 1104 22 0.43755620604476775 1104 23 0.5146847593508568 1104 24 0.5394424414276457 1104 25 0.36145329759202127 1105 1 0.1941308758099325 1105 2 0.3133880811341463 1105 3 0.3103500826562886 1105 4 0.2860299929493664 1105 5 0.32064494163992663 1105 6 0.29665367781661445 1105 7 0.2009624922315041 1105 8 0.24701844110298055 1105 9 0.24934380861302685 1105 10 0.4486545471161776 1105 11 0.5138736403600248 1105 12 0.5012821818651148 1105 13 0.38584691601204213 1105 14 0.5073209424258351 1105 15 0.48096867213916994 1105 16 0.2869912187516803 1105 17 0.3849333248553677 1105 18 0.431286611369714 1105 19 0.4155214447522084 1105 20 0.675145538851261 1105 21 0.511092623565709 1105 22 0.5692203309329642 1105 23 0.7462847499130305 1105 24 0.7218734970271754 1105 25 0.74994215130355 1106 1 0.15008462435952197 1106 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14 0.44551081542270765 1107 15 0.46957953389859597 1107 16 0.4811260515198157 1107 17 0.4962137873196255 1107 18 0.6578080722381188 1107 19 0.6842747694413518 1107 20 0.633210819122553 1107 21 0.6758455160452641 1107 22 0.5728608655429781 1107 23 0.5621011273317436 1107 24 0.46180280582430555 1107 25 0.4465414098978755 1108 1 0.12021890557418047 1108 2 0.1387095011020847 1108 3 0.21928084390289015 1108 4 0.10930031286743969 1108 5 0.12431398853434672 1108 6 0.2341997135626599 1108 7 -0.053882145283566274 1108 8 0.05324054083858698 1108 9 0.15506987060336996 1108 10 0.03726309366378899 1108 11 0.1553349409233391 1108 12 0.2414986461149411 1108 13 0.17645494639753423 1108 14 0.32817105020193793 1108 15 0.34772581587908746 1108 16 0.3819092028378961 1108 17 0.28118110382166606 1108 18 0.4011595304052875 1108 19 0.35253215230737567 1108 20 0.26274522307371484 1108 21 0.5265054491699956 1108 22 0.3973439626655022 1108 23 0.42330043403767803 1108 24 0.5239088015909132 1108 25 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-0.8319398876447579 2314 20 -0.9095245847130045 2314 21 -0.9203110985458949 2314 22 -0.7842945097511986 2314 23 -0.8183411389543735 2314 24 -0.7718164107897891 2314 25 -0.7967045258756613 2315 1 -0.33387204431829076 2315 2 -0.2659309468941018 2315 3 -0.4308841056775101 2315 4 -0.34125005072391446 2315 5 -0.3933084526205306 2315 6 -0.5216897443774897 2315 7 -0.4728404789775169 2315 8 -0.5000535916744937 2315 9 -0.6183879973345867 2315 10 -0.6431400158602116 2315 11 -0.5986215121896095 2315 12 -0.5213619438558368 2315 13 -0.5614353359814075 2315 14 -0.7872716479632018 2315 15 -0.918999918258443 2315 16 -0.7438199395899687 2315 17 -0.897681808623382 2315 18 -0.7795214006516957 2315 19 -0.8629488004115302 2315 20 -0.9411861679899474 2315 21 -0.8237455298819896 2315 22 -1.0190807177779178 2315 23 -0.8302926379068509 2315 24 -0.719527896519331 2315 25 -0.8130749714857473 2316 1 -1.5126027498568604 2316 2 -1.0456517067841737 2316 3 -0.7939931034770318 2316 4 -0.6460864324022708 2316 5 -0.6773737887976239 2316 6 -0.6615798251476193 2316 7 -0.7110511643477153 2316 8 -0.653624778612091 2316 9 -0.9201183774729316 2316 10 -0.880760107874373 2316 11 -0.6967552532934533 2316 12 -0.7064284898129785 2316 13 -0.7858144981241537 2316 14 -0.8773642178839793 2316 15 -0.8758191471565553 2316 16 -0.9007601062206589 2316 17 -1.0716027927496512 2316 18 -0.9828377444418663 2316 19 -0.7926353691144712 2316 20 -0.8225995325960724 2316 21 -1.1564636366325942 2316 22 -1.1072450497248099 2316 23 -0.9625291684316437 2316 24 -0.7203389807464404 2316 25 -0.8751595626574158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14814.0 3 1.0 4 258.0 5 2468.0 6 3208.0 7 2593.0 8 2171.0 9 1974.0 10 1878.0 11 1800.0 12 1795.0 13 1742.0 14 1661.0 15 1767.0 16 1730.0 17 1877.0 18 2075.0 19 2368.0 20 2881.0 21 3778.0 22 5002.0 23 6316.0 24 7704.0 25 9428.0 26 11133.0 27 12742.0 28 15217.0 29 19072.0 30 24768.0 31 32855.0 32 43096.0 33 57217.0 34 76723.0 35 104572.0 36 155512.0 37 283711.0 38 889187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.869757602605226 19.868460325497608 13.552821800035906 24.70896027186126 2 14.722919781704771 21.079644999097997 41.000191467627026 23.197243751570202 3 18.05517587906329 25.54476966887168 30.576107274995103 25.82394717706993 4 12.407821618576566 17.87577181928555 38.73013800056887 30.986268561569013 5 12.884655409863651 38.08049809803427 36.01462897276137 13.02021751934071 6 30.614843500116763 38.005881265722756 18.5089430876313 12.87033214652918 7 27.03937924645868 32.11874977173296 23.05840205324128 17.783468928567082 8 24.44864517285764 37.01926961187409 21.259713108449258 17.272372106819013 9 26.09216786730519 15.27214411646544 21.034544965563494 37.601143050665875 10 15.014714231799783 29.243691805738937 34.8103640430437 20.931229919417586 11 33.185490074119 22.755318760396527 25.448869842963344 18.61032132252113 12 22.89598659505261 25.25596344185748 32.21774849565103 19.630301467438883 13 28.440800533895853 22.526775032178737 26.271682602011847 22.76074183191356 14 20.514538812037447 22.8694799495765 28.47361565032035 28.142365588065697 15 22.450187981366767 31.497310045852622 23.98807145616111 22.064430516619502 16 21.442006581841667 28.67616663890569 28.429306073345423 21.452520705907222 17 21.140294860145627 28.94464814779973 28.48329970660076 21.431757285453884 18 20.905244447986046 27.591124303577242 30.693733357239143 20.809897891197572 19 22.825209978008893 27.19731237002613 28.60227525518872 21.37520239677626 20 23.065983285872235 27.380534714851578 29.121506684212335 20.431975315063855 21 22.539447311539966 26.81736533904711 28.317397988151143 22.325789361261783 22 22.662960532213596 28.310757492416 28.244241860135666 20.782040115234736 23 21.6552099669414 28.05847399194508 28.77952115385254 21.506794887260984 24 22.481066737502108 27.065116034543347 29.391285580283398 21.062531647671147 25 22.205498001486365 27.59603407240247 28.954182214197054 21.244285711914106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 389.0 2 559.0 3 729.0 4 729.0 5 729.0 6 1786.0 7 2843.0 8 2843.0 9 2843.0 10 3950.5 11 5058.0 12 5058.0 13 5058.0 14 7559.0 15 10060.0 16 10060.0 17 10060.0 18 17856.0 19 25652.0 20 25652.0 21 25652.0 22 39450.5 23 53249.0 24 53249.0 25 53249.0 26 75348.0 27 97447.0 28 97447.0 29 97447.0 30 123184.5 31 148922.0 32 148922.0 33 148922.0 34 166946.0 35 184970.0 36 184970.0 37 184970.0 38 196100.5 39 207231.0 40 207231.0 41 207231.0 42 216057.0 43 224883.0 44 224883.0 45 224883.0 46 229445.0 47 234007.0 48 234007.0 49 234007.0 50 222383.5 51 210760.0 52 210760.0 53 210760.0 54 185638.5 55 160517.0 56 160517.0 57 160517.0 58 135670.5 59 110824.0 60 110824.0 61 110824.0 62 89827.5 63 68831.0 64 68831.0 65 68831.0 66 52341.0 67 35851.0 68 35851.0 69 35851.0 70 25836.5 71 15822.0 72 15822.0 73 15822.0 74 10987.0 75 6152.0 76 6152.0 77 6152.0 78 4096.0 79 2040.0 80 2040.0 81 2040.0 82 1349.5 83 659.0 84 659.0 85 659.0 86 408.5 87 158.0 88 158.0 89 158.0 90 96.0 91 34.0 92 34.0 93 34.0 94 19.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4394348052729963 2 0.0 3 0.0 4 0.0 5 0.029992905737056288 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 5.5337464459513454E-5 17 0.0 18 1.1067492891902691E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004925034336896697 25 5.5337464459513454E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1807094.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.93456844031029 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13885459227932 59.80557625717321 2 11.955057376845888 17.199637861651137 3 2.7938360447481303 6.029201705158204 4 0.9448430906334102 2.7186751987409337 5 0.4019695888447812 1.445775444983915 6 0.21455981230023236 0.9260560501470715 7 0.11587808299235142 0.5834947924221681 8 0.07320758782255468 0.4212924989257268 9 0.052090687737716836 0.33724090279544605 >10 0.2699357156847038 3.4829036949747185 >50 0.01993369245543131 1.0003562077966186 >100 0.016221277744941195 2.3474474967845915 >500 0.0023058190917982792 1.1818102215483042 >1k 0.0011529095458991394 1.8218952243489919 >5k 1.537212727865519E-4 0.6986364425489762 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7516 0.4159163828777031 No Hit GGTATCAACGCAGAGTACTTTTTTT 5120 0.28332781803270884 No Hit TATCAACGCAGAGTACTTTTTTTTT 4984 0.275801922866215 No Hit GTCCTACAGTGGACATTTCTAAATT 3317 0.18355436961220611 No Hit CTGTAGGACGTGGAATATGGCAAGA 3288 0.18194958314288023 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3257 0.1802341217446353 No Hit CTTTAGGACGTGAAATATGGCGAGG 3233 0.178906022597607 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2348 0.1299323665509376 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1872 0.10359173346820919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.533746445951345E-5 0.0 0.0 0.0 3 0.0 5.533746445951345E-5 0.0 0.0 0.0 4 0.0 5.533746445951345E-5 0.0 0.0 0.0 5 0.0 1.106749289190269E-4 0.0 0.0 0.0 6 0.0 1.106749289190269E-4 0.0 0.0 0.0 7 0.0 1.106749289190269E-4 0.0 0.0 0.0 8 0.0 1.106749289190269E-4 0.0 0.0 0.0 9 0.0 1.106749289190269E-4 0.0 0.0 0.0 10 0.0 1.106749289190269E-4 0.0 0.0 0.0 11 0.0 1.106749289190269E-4 0.0 0.0 0.0 12 0.0 1.106749289190269E-4 0.0 0.0 0.0 13 0.0 1.106749289190269E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCTAT 65 1.8215724E-7 16.146877 1 GGTATCA 1435 0.0 13.829951 1 CGTGCAT 50 0.0015033049 13.295777 10 TAGGACC 510 0.0 12.852772 4 GTCCTAT 195 0.0 12.232483 1 AGGACCT 1165 0.0 10.437631 5 AATGTCC 580 0.0 10.31569 8 GGCGAGG 880 0.0 10.253277 19 GCACTCG 85 6.6133245E-4 10.055631 13 GTATTAC 135 6.07075E-7 9.894719 1 TGTCCAC 695 0.0 9.838602 10 GGACCTG 1120 0.0 9.836162 6 GTGGTAT 460 0.0 9.748757 1 ATAAACC 225 1.8189894E-12 9.710984 3 GTCCTAC 1585 0.0 9.691831 1 ATTTAGA 670 0.0 9.683745 1 TGGCGAG 1800 0.0 9.656069 18 GTCCTAA 1150 0.0 9.624304 1 GTATTAA 260 0.0 9.541336 1 TTTAGAA 700 0.0 9.499875 2 >>END_MODULE