FastQCFastQC Report
Thu 26 May 2016
SRR1511844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405885
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50100.20823937968772405No Hit
GTCCTACAGTGGACATTTCTAAATT49320.20499732946504093No Hit
CTGTAGGACGTGGAATATGGCAAGA46130.19173817534919582No Hit
GTCCTAAAGTGTGTATTTCTCATTT46020.19128096313830462No Hit
CTTTAGGACGTGAAATATGGCGAGG41360.17191179129509515No Hit
GGTATCAACGCAGAGTACTTTTTTT32100.13342283608734415No Hit
TATCAACGCAGAGTACTTTTTTTTT30010.12473580408041117No Hit
GTCCTACAGTGTGCATTTCTCATTT24950.10370404237941548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.014.0444031
CGGTAGG550.00210570712.7136921
TAGGACC9550.012.2644444
CTGCGTA550.003204139612.0216729
GCTGCGT658.4222463E-411.6253558
GACTGCG600.006133841411.0198677
GTATCAA21950.010.9677831
CTAGTAC1658.913048E-1110.8767523
AATCCCG1855.456968E-1210.72203319
CGTTAGG854.152381E-410.5769361
GGACCTG18350.010.4491396
ATACTGC2550.010.3714246
AGGACCT18650.010.3319175
GGCGAGG12350.010.325150519
TATGTCG1201.6410486E-610.23273416
GTCCTAG3600.09.9893291
TTCTACG800.002982029.9893282
AGGACGT28850.09.6256725
GTAGAAC6100.09.6004463
TGGCGAG29400.09.50985618
Produced by FastQC (version 0.11.5)