FastQCFastQC Report
Thu 26 May 2016
SRR1511844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405885
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66570.27669651708207166No Hit
GTCCTACAGTGGACATTTCTAAATT55230.22956209461383234No Hit
CTGTAGGACGTGGAATATGGCAAGA50120.20832250918061337No Hit
GTCCTAAAGTGTGTATTTCTCATTT48410.20121493753857728No Hit
CTTTAGGACGTGAAATATGGCGAGG46080.19153035161697254No Hit
GGTATCAACGCAGAGTACTTTTTTT44330.18425652098915782No Hit
TATCAACGCAGAGTACTTTTTTTTT44020.1829680138493735No Hit
GTCCTACAGTGTGCATTTCTCATTT26850.11160134420390001No Hit
CTGAAGGACCTGGAATATGGCGAGA24680.10258179422540978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.013.0160621
TCCAACG1352.7284841E-1112.66385418
TAGGACC9300.012.1559934
TATTCCG550.003069134712.0909895
CCAACGA1451.2223609E-911.1356919
CGAGTAA600.005890612511.07995110
ACCGTGC909.548627E-510.5523358
ATGTCGA1909.094947E-1210.49679617
GGCGAGG11550.010.44378519
CGTGCGC1106.070575E-610.36047410
TGTAGGA31850.010.1711812
GTCCTAA15800.010.0820761
GTAGGAC30600.09.9967983
TATGTCG2002.5465852E-119.97195616
AGGACGT28450.09.9508565
AGGACCT19150.09.9217365
TAGGACT4600.09.9131094
GGACCTG18450.09.8316866
GGACGTG27800.09.8045616
TGTCGAG1951.6370905E-109.74142618
Produced by FastQC (version 0.11.5)