##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511844_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2405885 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03600920243486 31.0 31.0 34.0 28.0 34.0 2 31.23337399751027 31.0 31.0 34.0 28.0 34.0 3 31.34947514116427 33.0 31.0 34.0 28.0 34.0 4 34.93186207985835 37.0 35.0 37.0 32.0 37.0 5 34.58879954777556 37.0 35.0 37.0 32.0 37.0 6 34.53295440139491 37.0 35.0 37.0 31.0 37.0 7 34.38955394792353 37.0 35.0 37.0 30.0 37.0 8 34.301419228267356 37.0 35.0 37.0 30.0 37.0 9 35.828466032250084 39.0 35.0 39.0 30.0 39.0 10 35.64468625890265 39.0 35.0 39.0 30.0 39.0 11 35.628972706509245 39.0 35.0 39.0 30.0 39.0 12 35.4714610216199 39.0 35.0 39.0 30.0 39.0 13 35.46102245119779 39.0 35.0 39.0 30.0 39.0 14 36.50951770346463 40.0 36.0 41.0 30.0 41.0 15 36.46153494452145 40.0 36.0 41.0 30.0 41.0 16 36.367106490958626 40.0 36.0 41.0 29.0 41.0 17 36.31224061000422 40.0 36.0 41.0 29.0 41.0 18 36.24457486538218 40.0 36.0 41.0 29.0 41.0 19 36.26380437967733 40.0 36.0 41.0 29.0 41.0 20 36.141402436109786 40.0 36.0 41.0 29.0 41.0 21 36.02551327266266 39.0 36.0 41.0 28.0 41.0 22 36.117797400956405 40.0 36.0 41.0 29.0 41.0 23 36.021681418687926 40.0 36.0 41.0 29.0 41.0 24 35.913406916789455 40.0 36.0 41.0 28.0 41.0 25 35.486163303732305 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47981241862687796 1101 2 0.33913994060900876 1101 3 0.41689907713982066 1101 4 0.38098058078249863 1101 5 0.5669676974604414 1101 6 0.525649875781383 1101 7 0.49995731091454587 1101 8 0.46920919565636865 1101 9 0.46531778298257365 1101 10 0.6105286726701991 1101 11 0.4884723159394966 1101 12 0.5375943625187602 1101 13 0.6201436900837791 1101 14 0.6805517688320748 1101 15 0.4677000186845319 1101 16 0.3483713467060028 1101 17 0.6469398181800301 1101 18 0.7129504403799558 1101 19 0.5602614972912505 1101 20 0.5991465221096135 1101 21 0.6684091179538996 1101 22 0.6488471894700467 1101 23 0.5623389026873511 1101 24 0.3357706634941948 1101 25 0.46393381310016224 1102 1 0.5193929972640845 1102 2 0.4335720425232594 1102 3 0.45253323895151 1102 4 0.4370637468764116 1102 5 0.5113489144040884 1102 6 0.4724828421947862 1102 7 0.5972705173472761 1102 8 0.5425871690502007 1102 9 0.46892923155561306 1102 10 0.8018830099322614 1102 11 0.7186244506751791 1102 12 0.7758073394028031 1102 13 0.8194611267229703 1102 14 1.0731240733129752 1102 15 1.068285510469103 1102 16 1.0699401559389585 1102 17 1.1656544126440167 1102 18 1.1485067016224733 1102 19 1.3983952731658178 1102 20 1.1941915089541908 1102 21 1.239522227734554 1102 22 0.8981550196308632 1102 23 1.118235812169992 1102 24 1.0449645247447634 1102 25 1.1296195562472633 1103 1 0.3107852101254913 1103 2 0.38479299702302683 1103 3 0.42773942730481807 1103 4 0.46472873108892543 1103 5 0.55924277144819 1103 6 0.5221109045383869 1103 7 0.5305279986731719 1103 8 0.27149414013282325 1103 9 0.5967777968634635 1103 10 0.6367386669083217 1103 11 0.5798475876643963 1103 12 0.600809686493406 1103 13 0.45375352259647883 1103 14 0.8078540644163823 1103 15 0.7955954438848849 1103 16 0.39236090657714584 1103 17 0.549292366501227 1103 18 0.7097228887849596 1103 19 0.6908515004340927 1103 20 0.5972780042216002 1103 21 0.564865563399394 1103 22 0.753665886761965 1103 23 0.8153192828402993 1103 24 0.9332495147853805 1103 25 1.0288261380176635 1104 1 0.25123296601249834 1104 2 0.28156743243907556 1104 3 0.36361578465615807 1104 4 0.32685476378902933 1104 5 0.4456952301401813 1104 6 0.40077753101014224 1104 7 0.3312736916334984 1104 8 0.4529969816040804 1104 9 0.5337154545512064 1104 10 0.4623209520819671 1104 11 0.4757323241094369 1104 12 0.45158026938150897 1104 13 0.5769196296262606 1104 14 0.5155956822307672 1104 15 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1107 14 0.6656703940202959 1107 15 0.6592160961079472 1107 16 0.7419131856509793 1107 17 0.8417156198141527 1107 18 0.7785786416709612 1107 19 0.7753874815918138 1107 20 0.7807920165432236 1107 21 0.7056150620184738 1107 22 0.7566196154777955 1107 23 0.851716903415543 1107 24 0.7662548221177801 1107 25 0.6520752283947715 1108 1 0.21544285315125578 1108 2 0.23090678466488868 1108 3 0.26160383101228746 1108 4 0.23742821334925424 1108 5 0.2805318842417819 1108 6 0.31329954792811066 1108 7 0.28802867839529966 1108 8 0.2601715519235981 1108 9 0.24288388818071383 1108 10 0.2915250615326954 1108 11 0.2955266552438829 1108 12 0.40895649784138755 1108 13 0.3297923465980759 1108 14 0.5781763174398833 1108 15 0.6287081971982644 1108 16 0.7220164277190193 1108 17 0.6263717767469785 1108 18 0.6855172286057467 1108 19 0.595003847381534 1108 20 0.4904515800778739 1108 21 0.5941669816809707 1108 22 0.7662418250302494 1108 23 0.8359263718026213 1108 24 0.6729576586476682 1108 25 0.7298260847907443 1109 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-0.5543241341258955 2315 2 -0.5308229021793522 2315 3 -0.6083867978863466 2315 4 -0.5263079240322384 2315 5 -0.6504396057633386 2315 6 -0.5408746165778382 2315 7 -0.5960286635464129 2315 8 -0.6218488690285753 2315 9 -0.7425199028034797 2315 10 -0.8245090078824475 2315 11 -0.7242216473219614 2315 12 -0.7981537534536045 2315 13 -0.8528182237742925 2315 14 -0.9125957348313136 2315 15 -1.0892228016001653 2315 16 -1.0295668859714127 2315 17 -1.147714253413021 2315 18 -1.0213653566684044 2315 19 -0.9411240480300762 2315 20 -0.8805825180829814 2315 21 -1.1440987321612397 2315 22 -1.1527895595356057 2315 23 -1.0116572638397585 2315 24 -1.1307551372175482 2315 25 -1.0095164433369348 2316 1 -1.4224407722223233 2316 2 -1.1877935714892978 2316 3 -1.0712460867046119 2316 4 -1.0175426947337414 2316 5 -1.0559100183243615 2316 6 -1.0527964524986757 2316 7 -1.1262672995326497 2316 8 -1.2089340151455374 2316 9 -1.386399442852479 2316 10 -1.2713333686287314 2316 11 -1.2442237932830835 2316 12 -1.4052382353445054 2316 13 -1.3315254354810193 2316 14 -1.5077274687423525 2316 15 -1.5415482314949358 2316 16 -1.729065139823284 2316 17 -1.7447270244364148 2316 18 -1.6439459771066396 2316 19 -1.7351638964843445 2316 20 -1.6960538758887864 2316 21 -1.9379947501259949 2316 22 -1.9337704681457666 2316 23 -1.7696357684625212 2316 24 -1.6594509804397575 2316 25 -1.8647177166071955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32247.0 3 1.0 4 589.0 5 5107.0 6 6758.0 7 5270.0 8 4306.0 9 3947.0 10 3714.0 11 3590.0 12 3385.0 13 3277.0 14 3246.0 15 3287.0 16 3390.0 17 3640.0 18 3801.0 19 4311.0 20 5341.0 21 6805.0 22 8944.0 23 11190.0 24 13661.0 25 16592.0 26 19185.0 27 21862.0 28 26035.0 29 31395.0 30 40423.0 31 51971.0 32 67790.0 33 89513.0 34 117694.0 35 158589.0 36 233994.0 37 422652.0 38 968383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22498962814067 20.22429093628882 13.828338841340186 24.722380594230327 2 14.440632033534436 21.139746912258897 41.677054389548964 22.742566664657705 3 18.320742678889474 25.692499849327792 30.498797739709087 25.487959732073644 4 12.387624512393568 18.01179191856635 39.12044840048465 30.48013516855544 5 12.65138084610293 38.393941107404316 36.34163093840325 12.6130471080895 6 30.460100960769115 38.36426096841703 18.664192178761663 12.511445892052198 7 27.13167919497399 31.960546742674733 23.142419525455292 17.765354536895988 8 24.042296285982083 38.01611465219659 21.200763960039655 16.740825101781674 9 25.991433505757755 15.445709167312652 21.09306970200155 37.46978762492804 10 14.503228541680087 29.61600408997105 35.284396386360946 20.596370981987917 11 33.21833753483645 22.59201915303516 25.82243124671379 18.3672120654146 12 22.985969819837607 25.126304873258697 32.65846040022694 19.22926490667675 13 28.514039532230345 22.645970193920327 26.304332916993122 22.53565735685621 14 20.108234599741884 23.18090848066304 28.524222895109286 28.18663402448579 15 22.12873848916303 32.32191064826457 23.804421242079318 21.744929620493085 16 21.1294148551696 28.802647510290402 29.017662122389158 21.05027551215084 17 20.9281823528556 29.413084997828243 28.618616434285098 21.04011621503106 18 20.65082938329529 27.833178989510714 31.189450530449513 20.32654109674448 19 22.88513374496287 27.271253613535144 28.446912466722225 21.39670017477976 20 23.05608954708974 27.885206483269148 28.99278228177988 20.065921687861223 21 22.242584329674944 27.27420471053271 28.018005848159827 22.465205111632518 22 22.526970324849277 28.913185792338368 28.19054111065159 20.369302772160765 23 21.44117445347554 28.49113735693934 28.770452453047422 21.297235736537697 24 22.40435818341963 27.38662375876693 29.559771433131885 20.649246624681545 25 22.01054664753024 28.02276752443905 28.98511689886832 20.981568929162393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 246.0 2 421.0 3 596.0 4 596.0 5 596.0 6 1561.5 7 2527.0 8 2527.0 9 2527.0 10 3753.0 11 4979.0 12 4979.0 13 4979.0 14 8375.5 15 11772.0 16 11772.0 17 11772.0 18 21790.0 19 31808.0 20 31808.0 21 31808.0 22 51550.0 23 71292.0 24 71292.0 25 71292.0 26 103216.0 27 135140.0 28 135140.0 29 135140.0 30 173274.0 31 211408.0 32 211408.0 33 211408.0 34 236151.5 35 260895.0 36 260895.0 37 260895.0 38 275726.5 39 290558.0 40 290558.0 41 290558.0 42 299522.5 43 308487.0 44 308487.0 45 308487.0 46 310253.5 47 312020.0 48 312020.0 49 312020.0 50 293667.5 51 275315.0 52 275315.0 53 275315.0 54 238163.5 55 201012.0 56 201012.0 57 201012.0 58 168754.0 59 136496.0 60 136496.0 61 136496.0 62 109186.5 63 81877.0 64 81877.0 65 81877.0 66 61847.0 67 41817.0 68 41817.0 69 41817.0 70 29960.5 71 18104.0 72 18104.0 73 18104.0 74 12285.5 75 6467.0 76 6467.0 77 6467.0 78 4378.0 79 2289.0 80 2289.0 81 2289.0 82 1443.5 83 598.0 84 598.0 85 598.0 86 371.5 87 145.0 88 145.0 89 145.0 90 87.0 91 29.0 92 29.0 93 29.0 94 17.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41477460477121725 2 0.0 3 0.0 4 0.0 5 0.028804369286146263 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 8.3129492889311E-5 17 0.0 18 4.15647464446555E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004655251601801416 25 8.3129492889311E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2405885.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.1172944751172 #Duplication Level Percentage of deduplicated Percentage of total 1 83.33046042145253 59.26236892539539 2 11.698103346838945 16.63874921034999 3 2.762388395953019 5.893607670289126 4 0.9489114259328361 2.699360532354755 5 0.4133446742711601 1.469797745993175 6 0.2160301009061912 0.9218085780980931 7 0.1267182824766736 0.6308302987192277 8 0.0902792644120522 0.5136333625751108 9 0.06156470985786129 0.3940484040213006 >10 0.310642756938391 3.9389940991759014 >50 0.019686776849960744 0.9812200774887045 >100 0.018133309006698812 2.627910336742233 >500 0.001868267551886238 0.9794725289694606 >1k 0.0016931174688926437 2.334374926817303 >5k 1.751500829888942E-4 0.7138233030102642 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6657 0.27669651708207166 No Hit GTCCTACAGTGGACATTTCTAAATT 5523 0.22956209461383234 No Hit CTGTAGGACGTGGAATATGGCAAGA 5012 0.20832250918061337 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4841 0.20121493753857728 No Hit CTTTAGGACGTGAAATATGGCGAGG 4608 0.19153035161697254 No Hit GGTATCAACGCAGAGTACTTTTTTT 4433 0.18425652098915782 No Hit TATCAACGCAGAGTACTTTTTTTTT 4402 0.1829680138493735 No Hit GTCCTACAGTGTGCATTTCTCATTT 2685 0.11160134420390001 No Hit CTGAAGGACCTGGAATATGGCGAGA 2468 0.10258179422540978 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.246942393339665E-4 0.0 5 0.0 0.0 0.0 1.246942393339665E-4 0.0 6 0.0 0.0 0.0 1.66258985778622E-4 0.0 7 0.0 0.0 0.0 2.49388478667933E-4 0.0 8 0.0 0.0 0.0 2.49388478667933E-4 0.0 9 0.0 0.0 0.0 3.32517971557244E-4 0.0 10 0.0 0.0 0.0 3.32517971557244E-4 0.0 11 0.0 0.0 0.0 3.32517971557244E-4 0.0 12 0.0 0.0 0.0 3.740827180018995E-4 0.0 13 0.0 0.0 0.0 4.15647464446555E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 13.016062 1 TCCAACG 135 2.7284841E-11 12.663854 18 TAGGACC 930 0.0 12.155993 4 TATTCCG 55 0.0030691347 12.090989 5 CCAACGA 145 1.2223609E-9 11.13569 19 CGAGTAA 60 0.0058906125 11.079951 10 ACCGTGC 90 9.548627E-5 10.552335 8 ATGTCGA 190 9.094947E-12 10.496796 17 GGCGAGG 1155 0.0 10.443785 19 CGTGCGC 110 6.070575E-6 10.360474 10 TGTAGGA 3185 0.0 10.171181 2 GTCCTAA 1580 0.0 10.082076 1 GTAGGAC 3060 0.0 9.996798 3 TATGTCG 200 2.5465852E-11 9.971956 16 AGGACGT 2845 0.0 9.950856 5 AGGACCT 1915 0.0 9.921736 5 TAGGACT 460 0.0 9.913109 4 GGACCTG 1845 0.0 9.831686 6 GGACGTG 2780 0.0 9.804561 6 TGTCGAG 195 1.6370905E-10 9.741426 18 >>END_MODULE