FastQCFastQC Report
Thu 26 May 2016
SRR1511843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169045
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46210.21304306734069603No Hit
GGTATCAACGCAGAGTACTTTTTTT29350.1353130064152657No Hit
TATCAACGCAGAGTACTTTTTTTTT28510.1314403343406891No Hit
GTCCTACAGTGGACATTTCTAAATT27620.12733714607119723No Hit
GTCCTAAAGTGTGTATTTCTCATTT25670.11834701446950156No Hit
CTGTAGGACGTGGAATATGGCAAGA24820.1144282391559419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.013.2911661
GTATTAG1900.012.6213991
TGTCGAG1157.665403E-811.49894318
GCGTACA709.782291E-411.419362
TATACCG752.1887099E-411.3344115
TAGGACC5600.011.3006784
CCGTGCG600.00613581111.0193129
AAACCGT803.9784788E-410.6260096
GTATCAA20700.010.6194531
GGCGAGG7200.010.49506719
ATTATAC2350.010.4501653
GTGGTAT3850.010.3812361
GTAGGAC15500.010.1156563
AGGACGT15950.010.0671365
GTCCTAG2600.09.99194051
CCCTTAA2001.0186341E-109.991941
TGGCGAG15700.09.98702518
TCCAACG1054.3738E-59.89534918
GTCCTAA8800.09.8783951
TTAGGAC10100.09.8194153
Produced by FastQC (version 0.11.5)