##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2169045 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.315264090878706 31.0 30.0 34.0 25.0 34.0 2 26.579174244886573 31.0 26.0 34.0 10.0 34.0 3 28.31549783430035 31.0 28.0 34.0 16.0 34.0 4 32.681179505266144 35.0 33.0 37.0 26.0 37.0 5 33.03878481082688 35.0 35.0 37.0 27.0 37.0 6 33.23112475766985 37.0 35.0 37.0 27.0 37.0 7 33.21031652178724 37.0 35.0 37.0 27.0 37.0 8 33.21898531381322 37.0 35.0 37.0 27.0 37.0 9 34.75483818915698 39.0 35.0 39.0 27.0 39.0 10 34.62720782648585 39.0 35.0 39.0 27.0 39.0 11 34.628307849767985 39.0 35.0 39.0 27.0 39.0 12 34.50026947343186 39.0 35.0 39.0 27.0 39.0 13 34.49141719051472 39.0 35.0 39.0 27.0 39.0 14 35.574694393154594 40.0 36.0 41.0 27.0 41.0 15 35.51267216678308 40.0 36.0 41.0 26.0 41.0 16 35.462070634772445 40.0 36.0 41.0 26.0 41.0 17 35.405770742423506 40.0 36.0 41.0 26.0 41.0 18 35.37021776864934 40.0 36.0 41.0 25.0 41.0 19 35.38544336332349 40.0 36.0 41.0 25.0 41.0 20 35.315881874281075 40.0 36.0 41.0 25.0 41.0 21 35.20840462046661 40.0 36.0 41.0 25.0 41.0 22 35.10623200532953 40.0 36.0 41.0 24.0 41.0 23 34.98930911991222 40.0 35.0 41.0 24.0 41.0 24 34.8673268650489 40.0 35.0 41.0 23.0 41.0 25 34.754335202819675 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9297074043681661 1101 2 3.446767517308725 1101 3 2.076210240855584 1101 4 1.153539515438652 1101 5 0.503299709099366 1101 6 0.5668042949302929 1101 7 0.33996140332711633 1101 8 0.5087942592120882 1101 9 0.5316370517221856 1101 10 0.45157897182887297 1101 11 0.37613894112566015 1101 12 0.3201143940022604 1101 13 0.3155861550999859 1101 14 0.3607923507901134 1101 15 0.48808090262349424 1101 16 0.4331199843939686 1101 17 0.60007691237778 1101 18 0.7224296436188951 1101 19 0.6483132001982028 1101 20 0.48211151529734764 1101 21 0.6606794454099116 1101 22 0.5642111212958554 1101 23 0.38746028994842874 1101 24 0.6006693290122058 1101 25 0.6449450365244687 1102 1 1.3110603758066475 1102 2 3.8335685439242013 1102 3 2.1547827237202455 1102 4 1.3682443061675897 1102 5 1.020485200632372 1102 6 1.0308958247793854 1102 7 0.9299038962372492 1102 8 0.8281567946255919 1102 9 0.9693154447909578 1102 10 0.9979123753801886 1102 11 0.9265378222237004 1102 12 0.9650731015329228 1102 13 0.9320730494161964 1102 14 1.0755316772994021 1102 15 1.001689863010526 1102 16 1.1503180865023737 1102 17 1.2297882878333013 1102 18 1.021002499068743 1102 19 1.1598921445497368 1102 20 1.0872142126707658 1102 21 1.0890159815427936 1102 22 1.211065714196387 1102 23 1.265620349676695 1102 24 1.082390156667202 1102 25 1.1166653912828792 1103 1 1.219285675510985 1103 2 3.718388085490254 1103 3 2.261316160813781 1103 4 1.3371961392260872 1103 5 0.968536448588381 1103 6 0.9068903124034904 1103 7 0.8908124706909533 1103 8 0.7130656289405479 1103 9 0.7843976259114811 1103 10 1.0553710152829865 1103 11 0.9801013078477041 1103 12 0.9368389001985094 1103 13 0.9514150199108897 1103 14 1.3214839927599087 1103 15 0.9311792771201652 1103 16 1.0416853022411203 1103 17 1.0247834707244792 1103 18 1.1198343674573081 1103 19 1.1911233201592708 1103 20 1.302453969230534 1103 21 1.2614239692353166 1103 22 1.2873178514860655 1103 23 1.2319956860795287 1103 24 1.336514832013414 1103 25 1.366355009134793 1104 1 1.3537845883065138 1104 2 3.8640972902090773 1104 3 2.292980075461429 1104 4 1.5592995084089125 1104 5 1.2379758528965965 1104 6 1.0421847470467043 1104 7 0.9424133504999261 1104 8 1.0991670957771476 1104 9 1.0649371890624693 1104 10 1.1592068903099317 1104 11 1.0019623319336262 1104 12 1.088959629075454 1104 13 1.0795976304991513 1104 14 1.2905258715831707 1104 15 1.3892821419405692 1104 16 1.316056104888915 1104 17 1.042638894504016 1104 18 1.0940807537319586 1104 19 1.1497012483389 1104 20 1.224584649324541 1104 21 1.2283829986829673 1104 22 1.2286224131407053 1104 23 1.0812467811319593 1104 24 1.168132862237492 1104 25 1.1245132282541093 1105 1 1.188253773228464 1105 2 3.792658915445937 1105 3 2.3104197508705617 1105 4 1.3863625375957227 1105 5 1.0123924807013012 1105 6 0.7989912479154384 1105 7 0.7333369665712652 1105 8 0.7886122846659731 1105 9 0.8452450938811609 1105 10 0.852439398982547 1105 11 0.8724838594941389 1105 12 0.6130786431031439 1105 13 0.8461043728039854 1105 14 0.9707858630433748 1105 15 1.0275662927525389 1105 16 1.024071655577707 1105 17 1.0539076343334983 1105 18 1.0753977414279632 1105 19 1.036588828055116 1105 20 0.9168109282767034 1105 21 1.148628409527717 1105 22 0.8770475689329089 1105 23 0.9197689245808149 1105 24 0.9000657382786486 1105 25 1.0344980966842243 1106 1 1.012816213198363 1106 2 3.6935646774429003 1106 3 2.013566601630412 1106 4 1.035211193735428 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0.8938259734688572 1107 18 0.74785049874707 1107 19 0.802224653185192 1107 20 0.9049781798654664 1107 21 0.8278115037976974 1107 22 0.9809001170401501 1107 23 0.9848945317745148 1107 24 1.007604676543508 1107 25 0.9511860968958956 1108 1 0.9631443935162629 1108 2 3.672949148659516 1108 3 2.001949038188492 1108 4 1.2907975894576893 1108 5 0.8340329092180028 1108 6 0.7494769472708853 1108 7 0.6505249094900449 1108 8 0.671652972741569 1108 9 0.5791177560624519 1108 10 0.7193624849621045 1108 11 0.534642962032585 1108 12 0.4714526325124879 1108 13 0.5367228040213377 1108 14 0.7278927141846339 1108 15 0.6072074475994356 1108 16 0.7050492308122003 1108 17 0.7097982899415882 1108 18 0.7113455615770121 1108 19 0.5578235712720385 1108 20 0.6273574781629989 1108 21 0.467066011696204 1108 22 0.4563558979115854 1108 23 0.5989942961458041 1108 24 0.5214138234204881 1108 25 0.48749380119281227 1109 1 0.8647229847494273 1109 2 3.036875904666907 1109 3 1.6343831879112294 1109 4 1.160728438414651 1109 5 0.8821077375781812 1109 6 0.7387065485383815 1109 7 0.8025557056347523 1109 8 0.6248968003095641 1109 9 0.7003771632449158 1109 10 0.6948584250202003 1109 11 0.7035580003214648 1109 12 0.8441643766371314 1109 13 0.8756998967068341 1109 14 0.6973119444460494 1109 15 0.7351459543329923 1109 16 0.7315609815383723 1109 17 0.8441592616870324 1109 18 0.640812322865635 1109 19 0.8121384973348853 1109 20 0.8626476154748559 1109 21 0.7705821524408591 1109 22 0.8294794125669824 1109 23 0.6694854500935179 1109 24 0.7609900050946337 1109 25 0.841689074496216 1110 1 0.7079364513287203 1110 2 3.3613530234960898 1110 3 1.7155826745866314 1110 4 0.8507964266191053 1110 5 0.8213920866389088 1110 6 0.5707347267821561 1110 7 0.45923029060635656 1110 8 0.3472195941059866 1110 9 0.34103765909463135 1110 10 0.1573488684452755 1110 11 0.15762364033894016 1110 12 0.3045059559293932 1110 13 0.2642675026482948 1110 14 0.3493338426548789 1110 15 0.4174485427995762 1110 16 0.5003246337882601 1110 17 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-1.606322441468322 2315 18 -1.627219552232809 2315 19 -1.5167008540065368 2315 20 -1.5742673186354992 2315 21 -1.7787945538646 2315 22 -1.6982227846294293 2315 23 -1.699244533630953 2315 24 -1.794059346509293 2315 25 -1.803810873002078 2316 1 -1.4934789182745547 2316 2 -1.2740501902236012 2316 3 -0.8310137098989898 2316 4 -1.0913212226906666 2316 5 -1.240438534580317 2316 6 -1.2285119081873717 2316 7 -1.204564341289128 2316 8 -1.4127433196070136 2316 9 -1.339218118298362 2316 10 -1.5699388898911621 2316 11 -1.6112034699600812 2316 12 -1.50580629593847 2316 13 -1.4137487477144361 2316 14 -1.7164817208302807 2316 15 -2.3350736754897383 2316 16 -1.9234442322259682 2316 17 -1.702217172098159 2316 18 -1.721677901326231 2316 19 -1.8597365026651147 2316 20 -2.08664200913411 2316 21 -2.023633827746835 2316 22 -1.9125891484340585 2316 23 -1.8985541745154464 2316 24 -1.9211007144048438 2316 25 -1.933323959914624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89699.0 3 2.0 4 817.0 5 4050.0 6 4834.0 7 4301.0 8 4070.0 9 3897.0 10 3626.0 11 3588.0 12 3525.0 13 3417.0 14 3449.0 15 3576.0 16 3716.0 17 4072.0 18 4629.0 19 5631.0 20 7118.0 21 9336.0 22 11349.0 23 13687.0 24 15976.0 25 17919.0 26 20452.0 27 22892.0 28 26467.0 29 31696.0 30 38765.0 31 49241.0 32 62514.0 33 81161.0 34 110081.0 35 156422.0 36 242587.0 37 323152.0 38 777331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55091756971386 18.69043749668633 12.591071185706152 25.167573747893655 2 16.631592500452346 21.250213980863407 39.05025172928239 23.067941789401864 3 18.479561281577837 25.022486854813984 29.830870267790665 26.667081595817514 4 12.466269717779022 16.81989078142685 38.380577627481216 32.33326187331291 5 13.329817171899725 37.73010264486646 35.71759199495443 13.22248818827938 6 31.523252023824398 37.08410691011726 18.33693938830919 13.055701677749157 7 27.789188329426086 31.576799928078948 22.692567466327347 17.941444276167623 8 25.221745053698747 36.276195284099686 20.868861641874652 17.63319802032692 9 26.60954475356666 15.070779997648733 20.26855136707629 38.05112388170831 10 15.168288348097898 28.70180194509565 34.515835310009706 21.61407439679675 11 34.21934538010968 22.298523082739177 24.76988720842583 18.712244328725312 12 23.209108155893492 24.6148881189648 31.798510404348455 20.377493320793253 13 28.891431743337264 21.879070059072188 25.898173432406303 23.331324765184245 14 21.288769942532312 21.828823283979816 27.65705644650065 29.225350326987222 15 23.16046923876637 30.37424304244495 23.54745982679013 22.917827891998552 16 22.518527739166316 27.877014999688804 27.34479920886842 22.259658052276464 17 22.04937573794133 28.22924970067417 27.612940465394615 22.10843409598989 18 21.84293087510863 27.30538093953791 29.309673151087228 21.54201503426623 19 23.47681603214691 26.80473683773758 27.568576357673113 22.149870772442394 20 23.703009763300926 26.98575084565022 27.9847176701593 21.326521720889556 21 23.388536018518817 26.535001477614262 27.130980246072333 22.945482257794588 22 23.488150574974433 27.45096050376078 27.276868332652516 21.784020588612268 23 22.752763662483254 27.270380195496447 27.61905947418261 22.35779666783769 24 23.199981927205364 26.61269724472869 28.12707756456183 22.060243263504113 25 23.022694528647662 26.889270524774954 27.832735906686185 22.255299039891195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 285.0 1 285.0 2 422.5 3 560.0 4 560.0 5 560.0 6 1385.5 7 2211.0 8 2211.0 9 2211.0 10 3202.5 11 4194.0 12 4194.0 13 4194.0 14 6927.5 15 9661.0 16 9661.0 17 9661.0 18 17369.5 19 25078.0 20 25078.0 21 25078.0 22 39570.0 23 54062.0 24 54062.0 25 54062.0 26 76807.5 27 99553.0 28 99553.0 29 99553.0 30 128379.5 31 157206.0 32 157206.0 33 157206.0 34 179682.5 35 202159.0 36 202159.0 37 202159.0 38 220391.5 39 238624.0 40 238624.0 41 238624.0 42 252790.0 43 266956.0 44 266956.0 45 266956.0 46 273707.0 47 280458.0 48 280458.0 49 280458.0 50 272724.5 51 264991.0 52 264991.0 53 264991.0 54 238313.0 55 211635.0 56 211635.0 57 211635.0 58 183060.5 59 154486.0 60 154486.0 61 154486.0 62 127230.5 63 99975.0 64 99975.0 65 99975.0 66 77465.0 67 54955.0 68 54955.0 69 54955.0 70 40421.0 71 25887.0 72 25887.0 73 25887.0 74 18110.5 75 10334.0 76 10334.0 77 10334.0 78 7186.0 79 4038.0 80 4038.0 81 4038.0 82 2650.0 83 1262.0 84 1262.0 85 1262.0 86 814.0 87 366.0 88 366.0 89 366.0 90 225.0 91 84.0 92 84.0 93 84.0 94 50.5 95 17.0 96 17.0 97 17.0 98 12.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.46913503408182 3 0.0 4 0.0 5 5.993421067797118E-4 6 3.6882591186443805E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.8441295593221902E-4 14 0.0 15 0.0 16 0.0 17 1.8441295593221902E-4 18 0.0 19 1.3830971694916427E-4 20 1.8441295593221902E-4 21 3.6882591186443805E-4 22 0.0036421558796613258 23 1.3830971694916427E-4 24 0.0017980263203391353 25 9.220647796610951E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2169045.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.05017989681284 #Duplication Level Percentage of deduplicated Percentage of total 1 85.45496525456412 64.13410515430927 2 10.010931433241579 15.026444099988776 3 2.281411337393225 5.1366099386996975 4 0.878339549520973 2.6367816480813824 5 0.41791178625288194 1.5682177369638592 6 0.24892320206628374 1.1209038657339172 7 0.1674848604523773 0.8798838234860455 8 0.10560318690181626 0.6340430539726447 9 0.08032497760701565 0.5425563617652588 >10 0.32789257372598196 4.086973067758083 >50 0.013685809970857428 0.7089326254051019 >100 0.010357987215903008 1.5188690916369196 >500 0.0013008246521717059 0.6715816360050213 >1k 8.672164347801321E-4 1.3340978961940642 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4621 0.21304306734069603 No Hit GGTATCAACGCAGAGTACTTTTTTT 2935 0.1353130064152657 No Hit TATCAACGCAGAGTACTTTTTTTTT 2851 0.1314403343406891 No Hit GTCCTACAGTGGACATTTCTAAATT 2762 0.12733714607119723 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2567 0.11834701446950156 No Hit CTGTAGGACGTGGAATATGGCAAGA 2482 0.1144282391559419 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6103238983054756E-5 2 0.0 0.0 0.0 0.0 4.6103238983054756E-5 3 0.0 0.0 0.0 0.0 4.6103238983054756E-5 4 0.0 0.0 0.0 0.0 4.6103238983054756E-5 5 0.0 0.0 0.0 0.0 4.6103238983054756E-5 6 0.0 0.0 0.0 0.0 9.220647796610951E-5 7 0.0 0.0 0.0 0.0 9.220647796610951E-5 8 0.0 0.0 0.0 0.0 9.220647796610951E-5 9 0.0 0.0 0.0 0.0 1.3830971694916427E-4 10 0.0 0.0 0.0 0.0 1.3830971694916427E-4 11 0.0 0.0 0.0 4.6103238983054756E-5 1.3830971694916427E-4 12 0.0 0.0 0.0 4.6103238983054756E-5 1.3830971694916427E-4 13 0.0 0.0 0.0 4.6103238983054756E-5 1.3830971694916427E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1060 0.0 13.291166 1 GTATTAG 190 0.0 12.621399 1 TGTCGAG 115 7.665403E-8 11.498943 18 GCGTACA 70 9.782291E-4 11.41936 2 TATACCG 75 2.1887099E-4 11.334411 5 TAGGACC 560 0.0 11.300678 4 CCGTGCG 60 0.006135811 11.019312 9 AAACCGT 80 3.9784788E-4 10.626009 6 GTATCAA 2070 0.0 10.619453 1 GGCGAGG 720 0.0 10.495067 19 ATTATAC 235 0.0 10.450165 3 GTGGTAT 385 0.0 10.381236 1 GTAGGAC 1550 0.0 10.115656 3 AGGACGT 1595 0.0 10.067136 5 GTCCTAG 260 0.0 9.9919405 1 CCCTTAA 200 1.0186341E-10 9.99194 1 TGGCGAG 1570 0.0 9.987025 18 TCCAACG 105 4.3738E-5 9.895349 18 GTCCTAA 880 0.0 9.878395 1 TTAGGAC 1010 0.0 9.819415 3 >>END_MODULE