FastQCFastQC Report
Thu 26 May 2016
SRR1511843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169045
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54890.25306067877798755No Hit
TATCAACGCAGAGTACTTTTTTTTT36880.17002874536950593No Hit
GGTATCAACGCAGAGTACTTTTTTT36690.1691527838288279No Hit
GTCCTACAGTGGACATTTCTAAATT29150.1343909416356046No Hit
CTGTAGGACGTGGAATATGGCAAGA28980.13360718657289267No Hit
CTTTAGGACGTGAAATATGGCGAGG25930.11954569868306097No Hit
GTCCTAAAGTGTGTATTTCTCATTT25590.11797818855763711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11150.013.6020231
GTTCTAG2250.011.0222971
TAGGACC5350.010.8320664
GTATCAA25950.010.5125941
TGGCCCG1106.0456914E-610.3639135
GTAAGAC2400.010.2919413
GTCCTAC13700.010.2347681
ACTATAC1502.4447218E-810.1336043
TAAGCGA951.6494244E-49.9970413
AATCCCG1356.471E-79.84893619
GTCCTAA9050.09.5912251
GTCTAAA2303.6379788E-129.5385261
GTAGGAC16050.09.529853
AAGACCG900.00111361539.5002545
CCGGAAC800.00451566939.5002543
TACGGTG800.00451566939.5002545
TAAGACG900.00111361539.5002544
GGAGCGT1106.860218E-59.4971886
GGCGAGG6500.09.49718819
GTCCTAT2252.5465852E-119.3265591
Produced by FastQC (version 0.11.5)