FastQCFastQC Report
Thu 26 May 2016
SRR1511842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033660
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48220.23710944799032285No Hit
GGTATCAACGCAGAGTACTTTTTTT31260.1537130100410098No Hit
TATCAACGCAGAGTACTTTTTTTTT30850.1516969404915276No Hit
GTCCTACAGTGGACATTTCTAAATT27750.1364534878003206No Hit
GTCCTAAAGTGTGTATTTCTCATTT26520.13040527915187397No Hit
CTGTAGGACGTGGAATATGGCAAGA26060.12814334746221098No Hit
CTTTAGGACGTGAAATATGGCGAGG24720.12155424210536668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.013.3272281
CGTTAGG751.2925518E-411.9945051
TCCAACG951.1149423E-611.93131218
TAGGACC4850.011.4897024
GTCCTAG2150.011.1576791
GCGAAAA600.006137675611.01880713
AACTTCG600.006137675611.0188077
GTCCTAA9400.010.7397591
TAATACT2850.010.6048284
TCCTAGA2650.010.5611992
GGCGAGG8400.010.45765219
CTGGTCG1002.559007E-510.3891629
GTCTAAG1654.440153E-910.2983121
GTATCAA21150.010.1608291
GGACGTG17200.09.9388926
AGGACTG3900.09.929285
AGGACCT11100.09.8703725
GGACCTG10950.09.8328316
GACGTGA9150.09.8059657
TGGCGAG16300.09.79331418
Produced by FastQC (version 0.11.5)