FastQCFastQC Report
Thu 26 May 2016
SRR1511842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033660
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61560.30270546699054907No Hit
TATCAACGCAGAGTACTTTTTTTTT41170.202442886224836No Hit
GGTATCAACGCAGAGTACTTTTTTT40890.20106605823982376No Hit
GTCCTACAGTGGACATTTCTAAATT31470.154745631029769No Hit
GTCCTAAAGTGTGTATTTCTCATTT29620.14564873184308094No Hit
CTGTAGGACGTGGAATATGGCAAGA29310.14412438657396023No Hit
CTTTAGGACGTGAAATATGGCGAGG28090.13812535035354975No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20380.1002134083376769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.013.4860311
TAGGACC5450.012.3752664
GTACGGA550.0029807412.1381051
GGCGAGG7100.011.63858519
TCCAACG1303.2769094E-810.95941118
TGGCGAG16400.010.42480518
TAGGACT2750.010.3628994
GTATCAA27100.010.3113111
AGGACCT11700.010.3112325
GTCCTAA11400.010.0390341
GTTCTAG3000.09.8549851
GTCCTAG2350.09.7399991
GTAGGAC18350.09.68051053
TGTAGGA19300.09.6469812
TTAGGAC10750.09.6318723
GGACCTG12050.09.6156816
TAGACCC1304.242982E-69.4993244
CTAGTAC900.00111454989.4993243
TAAGGGT1701.66674E-89.4993244
CTTACAC2800.09.4993243
Produced by FastQC (version 0.11.5)