FastQCFastQC Report
Thu 26 May 2016
SRR1511841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361741
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66090.2798359345923198No Hit
CTGTAGGACGTGGAATATGGCAAGA62680.26539743350350437No Hit
GTCCTAAAGTGTGTATTTCTCATTT61980.2624335183239822No Hit
GTATCAACGCAGAGTACTTTTTTTT57910.24520046863733153No Hit
CTTTAGGACGTGAAATATGGCGAGG54880.23237095007454245No Hit
GGTATCAACGCAGAGTACTTTTTTT38730.16398919271842255No Hit
TATCAACGCAGAGTACTTTTTTTTT37050.15687579628756923No Hit
GTCCTACAGTGTGCATTTCTCATTT31510.13341852472392188No Hit
CTGAAGGACCTGGAATATGGCGAGA29720.12583937019342933No Hit
ATTTAGAAATGTCCACTGTAGGACG25400.10754777937123504No Hit
CTGTAGGACCTGGAATATGGCGAGA23670.10022267471327295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1108.367351E-1114.5377961
GGTATCA12150.014.0666641
CCAACGA1052.1638698E-812.5933319
GGCGAGG17150.012.39139619
TAGGACC11850.012.2745114
AGGACCG803.0433615E-511.8062475
CGTAGGA853.1719173E-511.75851252
TCCAACG1900.011.43341718
ATAGGAC3850.011.2849323
ATTGCGA600.006136699611.01916317
CCACCTT10600.010.69245113
TATGTCG2300.010.67695316
GTCAACG750.001736618110.6610512
CACCTTT10650.010.6422514
TGGCGAG35800.010.60583518
AGGACGT41200.010.5912355
CGTTAGG854.1316223E-410.5826621
TTAGGAC24150.010.52051453
GACGTGA21750.010.5089167
GGACGTG40550.010.4582096
Produced by FastQC (version 0.11.5)