##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511841_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2361741 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.547985575048237 31.0 30.0 34.0 25.0 34.0 2 26.80838711780843 31.0 26.0 34.0 10.0 34.0 3 28.550320716793248 31.0 28.0 34.0 16.0 34.0 4 32.931836725534254 35.0 33.0 37.0 27.0 37.0 5 33.30359340842201 35.0 35.0 37.0 28.0 37.0 6 33.50750061077824 37.0 35.0 37.0 28.0 37.0 7 33.49949465246189 37.0 35.0 37.0 28.0 37.0 8 33.51355546607355 37.0 35.0 37.0 28.0 37.0 9 35.0697248343489 39.0 35.0 39.0 28.0 39.0 10 34.93832346561287 39.0 35.0 39.0 28.0 39.0 11 34.94223329315111 39.0 35.0 39.0 28.0 39.0 12 34.82279005191509 39.0 35.0 39.0 27.0 39.0 13 34.82152742404862 39.0 35.0 39.0 27.0 39.0 14 35.914128602585976 40.0 36.0 41.0 27.0 41.0 15 35.87053618495847 40.0 36.0 41.0 27.0 41.0 16 35.82344550058622 40.0 36.0 41.0 27.0 41.0 17 35.78775064666278 40.0 36.0 41.0 27.0 41.0 18 35.75808312596512 40.0 36.0 41.0 27.0 41.0 19 35.789478185795986 40.0 36.0 41.0 27.0 41.0 20 35.73016643230566 40.0 36.0 41.0 27.0 41.0 21 35.62137931297293 40.0 36.0 41.0 27.0 41.0 22 35.52413664326444 40.0 36.0 41.0 27.0 41.0 23 35.41850905751308 40.0 36.0 41.0 26.0 41.0 24 35.312005423117945 40.0 36.0 41.0 25.0 41.0 25 35.21244962932006 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1680973353600592 1101 2 3.659371033266943 1101 3 2.3200315473395605 1101 4 1.3692159977398717 1101 5 0.691114967250698 1101 6 0.7685421429819925 1101 7 0.6137076413791078 1101 8 0.5054673237392038 1101 9 0.7065996231773255 1101 10 0.6261895614117776 1101 11 0.6376825838848319 1101 12 0.6383453054043287 1101 13 0.569039539349049 1101 14 0.6426998990174866 1101 15 0.7502954605390357 1101 16 0.5967623532159152 1101 17 0.7908714243648589 1101 18 0.8478015712536546 1101 19 0.9680803402439011 1101 20 0.9093464987509776 1101 21 0.956783335421548 1101 22 0.88683535267905 1101 23 0.8851654451806894 1101 24 0.8765095732295336 1101 25 0.9884106799917092 1102 1 1.1761693288151989 1102 2 3.8687519986337264 1102 3 2.203850373727242 1102 4 1.375803002227066 1102 5 0.9438680227580463 1102 6 0.8611816550924587 1102 7 0.7587085090688248 1102 8 0.7262001496806434 1102 9 0.7148848203908074 1102 10 0.7421971970812606 1102 11 0.705414442972021 1102 12 0.7343663282865407 1102 13 0.7927869672688246 1102 14 0.8081360990323603 1102 15 0.766493987945637 1102 16 0.8217989201395852 1102 17 0.9720847024965948 1102 18 0.9117230329389585 1102 19 0.9199012490868981 1102 20 1.0292860083823356 1102 21 0.9602987183206224 1102 22 1.1365217448252238 1102 23 1.0993658071312566 1102 24 0.7434976982760162 1102 25 0.7624419664036921 1103 1 1.1628669066082864 1103 2 3.7276823208136136 1103 3 2.134187458289574 1103 4 1.4313459302109663 1103 5 0.9919467773058912 1103 6 0.8976895599401402 1103 7 0.9044330483225806 1103 8 0.7045074915261509 1103 9 0.7433577904343807 1103 10 0.9453904359701042 1103 11 1.0081281677883283 1103 12 0.8981603746142213 1103 13 0.9474527770628214 1103 14 1.213127893789732 1103 15 0.8506096157820409 1103 16 0.9303834209875106 1103 17 1.0160675209136016 1103 18 1.1768568122581513 1103 19 1.2101553233783733 1103 20 1.2682204381152289 1103 21 1.2385398439567297 1103 22 1.2011883064285414 1103 23 1.2514259290821812 1103 24 1.2934523595705727 1103 25 1.321769914619587 1104 1 1.3161120224431144 1104 2 3.861731365251739 1104 3 2.288797647592439 1104 4 1.5376561719395099 1104 5 1.2852622634824584 1104 6 0.9051070929329015 1104 7 0.8735033628745441 1104 8 0.9523024893770966 1104 9 1.0201177123955603 1104 10 1.0482812031380462 1104 11 0.9829749182845333 1104 12 1.0289211653914805 1104 13 1.0485154365050917 1104 14 1.2478799103185523 1104 15 1.2995240449199343 1104 16 1.351386872222541 1104 17 1.2339061246396525 1104 18 1.2013907325473028 1104 19 1.156553257443214 1104 20 1.1356534794124116 1104 21 1.1860556630749883 1104 22 1.207412523245587 1104 23 1.2440037991789268 1104 24 1.2888098978664928 1104 25 1.308909430644313 1105 1 1.1167470799063217 1105 2 3.769289125879059 1105 3 2.1356426088917466 1105 4 1.3806587031165662 1105 5 0.8983082200282482 1105 6 0.8276127779669906 1105 7 0.5843669553033664 1105 8 0.6589098669230466 1105 9 0.808181003438456 1105 10 0.809321505293056 1105 11 0.7243584414795095 1105 12 0.6164979288736347 1105 13 0.7420911977709181 1105 14 0.9207065489242297 1105 15 0.9843965066828986 1105 16 0.9665944836189695 1105 17 0.8626660882201804 1105 18 0.9273328339249716 1105 19 0.9338819783916605 1105 20 1.0009449092610723 1105 21 0.9549100080352844 1105 22 0.9201497635135354 1105 23 1.0392973079655405 1105 24 1.0029064591268622 1105 25 1.107403462410005 1106 1 0.782586621817309 1106 2 3.541766498213896 1106 3 1.9427120133266627 1106 4 0.8860300189589267 1106 5 0.6865747134541493 1106 6 0.502560241588192 1106 7 0.4523075997731141 1106 8 0.41642010093912063 1106 9 0.4599697084696075 1106 10 0.5695846102988185 1106 11 0.3908498399697038 1106 12 0.39837234929864707 1106 13 0.28987373947386885 1106 14 0.5397146691443879 1106 15 0.5572228580842804 1106 16 0.617263705410636 1106 17 0.6048874426714903 1106 18 0.6361362914533615 1106 19 0.3838750146770167 1106 20 0.5806591677753161 1106 21 0.5679857485690363 1106 22 0.31412368219850606 1106 23 0.3439131048436863 1106 24 0.588845749638935 1106 25 0.47431144970254735 1107 1 1.0975600170188855 1107 2 3.5894017064322874 1107 3 2.1006045326241534 1107 4 1.5142229301594412 1107 5 1.1364395932755897 1107 6 0.9613511025506583 1107 7 0.7393866226483823 1107 8 0.7569351087956235 1107 9 0.755061194431029 1107 10 0.8414591543782635 1107 11 0.8224217198800261 1107 12 0.8030725862515666 1107 13 0.8468350908265805 1107 14 1.1191018616128758 1107 15 1.1211313905693032 1107 16 1.0170186192909085 1107 17 0.9587930991887035 1107 18 0.9464928359990026 1107 19 1.003823205332722 1107 20 1.161331322537599 1107 21 0.986799189651947 1107 22 1.1801008572434881 1107 23 1.195672476054824 1107 24 1.227448951605659 1107 25 1.2426973106733996 1108 1 0.9898046084877237 1108 2 3.611504123695564 1108 3 2.052315185304767 1108 4 1.2649338011872544 1108 5 0.7912090594610675 1108 6 0.6313557002681307 1108 7 0.5367462191853605 1108 8 0.6679465533842546 1108 9 0.683508994361091 1108 10 0.6511580305114961 1108 11 0.7277348350910131 1108 12 0.670610925712225 1108 13 0.7413323863391028 1108 14 0.9009009360337643 1108 15 0.815337201445125 1108 16 0.7178069769471733 1108 17 0.8237196155922604 1108 18 0.7429147822003799 1108 19 0.7433781320799042 1108 20 0.7137648287143463 1108 21 0.7539425514532567 1108 22 0.5919864007061193 1108 23 0.728099520574581 1108 24 0.8375053490898736 1108 25 0.7932734455019101 1109 1 0.5974069952382415 1109 2 2.7014011056864753 1109 3 1.5492698922906385 1109 4 0.9427277346653113 1109 5 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1110 17 0.1144924114313639 1110 18 0.16730952101832486 1110 19 0.02505443423611098 1110 20 0.0482761489925565 1110 21 -0.03597874923544708 1110 22 0.06930216036265335 1110 23 0.053025292039421856 1110 24 -0.07535512856648552 1110 25 0.023572680350760322 1111 1 -0.18648328072450227 1111 2 2.4615879585943254 1111 3 0.9602777169658196 1111 4 0.2312481404374438 1111 5 -0.23088411194435565 1111 6 -0.4262279180359059 1111 7 -0.3835961873434002 1111 8 -0.5379548459025969 1111 9 -0.8487483303628167 1111 10 -0.6519879441392789 1111 11 -0.6281248590942283 1111 12 -0.7046580339705741 1111 13 -0.6377680065892051 1111 14 -0.9520484045839481 1111 15 -0.8950831282205414 1111 16 -0.7827116612411089 1111 17 -0.861818821901899 1111 18 -0.8042479968033476 1111 19 -0.8010606497574031 1111 20 -0.7001273020171368 1111 21 -0.6938181988648395 1111 22 -0.79521769267933 1111 23 -0.8271739238930849 1111 24 -0.7900624875280542 1111 25 -1.0963206644806434 1112 1 0.7844433419562797 1112 2 3.459686277413077 1112 3 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-1.45852166619202 2315 17 -1.4163409391100004 2315 18 -1.4360946864600592 2315 19 -1.4638475327565885 2315 20 -1.4918254472244286 2315 21 -1.6919804516833494 2315 22 -1.5560801447334427 2315 23 -1.6105989946876775 2315 24 -1.6036474264810963 2315 25 -1.6777297381806875 2316 1 -1.352309624985967 2316 2 -1.2641148144911085 2316 3 -0.8491103331293495 2316 4 -0.9077121069456737 2316 5 -1.1252068516404634 2316 6 -1.2828163337540843 2316 7 -1.265660266244133 2316 8 -1.3556978231877395 2316 9 -1.360873780673728 2316 10 -1.5769234420319123 2316 11 -1.6102664852710689 2316 12 -1.3952147362780352 2316 13 -1.3749674392372242 2316 14 -1.5884477001090076 2316 15 -2.1043037014226016 2316 16 -1.7674494228950905 2316 17 -1.8078639179861398 2316 18 -1.7575529568050925 2316 19 -1.6525720190823563 2316 20 -1.984809357468528 2316 21 -1.9877649396077146 2316 22 -1.9389134688822764 2316 23 -1.8751133604879726 2316 24 -1.8279633425258268 2316 25 -1.9902666313272093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85428.0 3 3.0 4 760.0 5 3832.0 6 4623.0 7 4094.0 8 3747.0 9 3814.0 10 3716.0 11 3458.0 12 3353.0 13 3399.0 14 3388.0 15 3496.0 16 3687.0 17 3907.0 18 4422.0 19 5629.0 20 7225.0 21 9447.0 22 11512.0 23 13853.0 24 16077.0 25 18330.0 26 20763.0 27 23328.0 28 26962.0 29 32553.0 30 40685.0 31 51801.0 32 67102.0 33 88354.0 34 120448.0 35 172653.0 36 267900.0 37 361291.0 38 866701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85912003051986 19.785573439255195 13.077132505215433 25.278174025009513 2 15.283268932985406 21.525696814154216 41.65265750007169 21.53837675278869 3 18.11299376180538 25.659291175450655 30.36416778977881 25.863547272965153 4 11.594074032673355 17.710282372199153 40.011923407350764 30.683720187776732 5 12.179561820650736 38.68859525254189 36.80590517024195 12.325937756565429 6 29.88766661585624 38.524507562285116 19.1406686652101 12.447157156648544 7 26.65859634904928 32.116688493784885 23.6137662851261 17.610948872039735 8 23.42966481083235 38.79443173489388 21.510063973992068 16.265839480281706 9 26.12293219281877 15.25273093027559 21.382446254690926 37.24189062221471 10 14.421818480519244 29.512423250474967 35.930866255021186 20.134892013984597 11 32.78386580069533 22.40440420859019 26.665625062189292 18.146104928525187 12 23.16452142720137 25.026961042722295 33.25301123196828 18.55550629810805 13 28.67976888206905 22.91316818376975 26.37193456683172 22.035128367329484 14 19.568657189759588 23.742654253789894 28.84562701837331 27.843061538077208 15 21.613801005275345 33.43795953917047 23.800874016244798 21.14736543930939 16 20.34511828350357 28.827377769196538 30.184935604708564 20.642568342591332 17 20.421327274490235 29.86069092659736 29.214680612958226 20.503301185954175 18 19.99986450673465 27.8773159292234 32.41900784209615 19.7038117219458 19 22.769202628238403 27.32009616663011 28.59305916754385 21.317642037587635 20 22.70385577358302 28.249660947838684 29.63131702975487 19.415166248823425 21 21.738018498351206 27.40929930847127 28.185893017134937 22.666789176042588 22 22.145759479413492 29.711040275333623 28.437435955630907 19.705764289621975 23 20.73661069653848 29.28248542597479 29.304503128207386 20.676400749279345 24 22.225962462664636 27.55144383160958 30.14542054840111 20.077173157324676 25 21.55904820336678 28.480308656010887 29.526028242939585 20.434614897682753 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 317.0 2 483.5 3 650.0 4 650.0 5 650.0 6 1645.0 7 2640.0 8 2640.0 9 2640.0 10 4008.0 11 5376.0 12 5376.0 13 5376.0 14 9000.0 15 12624.0 16 12624.0 17 12624.0 18 22969.5 19 33315.0 20 33315.0 21 33315.0 22 53960.0 23 74605.0 24 74605.0 25 74605.0 26 107988.0 27 141371.0 28 141371.0 29 141371.0 30 182278.0 31 223185.0 32 223185.0 33 223185.0 34 247778.5 35 272372.0 36 272372.0 37 272372.0 38 283455.0 39 294538.0 40 294538.0 41 294538.0 42 300709.0 43 306880.0 44 306880.0 45 306880.0 46 305504.0 47 304128.0 48 304128.0 49 304128.0 50 282444.5 51 260761.0 52 260761.0 53 260761.0 54 221245.5 55 181730.0 56 181730.0 57 181730.0 58 150648.5 59 119567.0 60 119567.0 61 119567.0 62 94427.5 63 69288.0 64 69288.0 65 69288.0 66 52103.0 67 34918.0 68 34918.0 69 34918.0 70 24973.0 71 15028.0 72 15028.0 73 15028.0 74 10347.0 75 5666.0 76 5666.0 77 5666.0 78 3814.0 79 1962.0 80 1962.0 81 1962.0 82 1268.0 83 574.0 84 574.0 85 574.0 86 373.5 87 173.0 88 173.0 89 173.0 90 113.0 91 53.0 92 53.0 93 53.0 94 35.0 95 17.0 96 17.0 97 17.0 98 10.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.498867149276741 3 0.0 4 0.0 5 5.92783035904445E-4 6 8.891745538566676E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.2702493626523824E-4 14 0.0 15 0.0 16 0.0 17 5.92783035904445E-4 18 0.0 19 2.963915179522225E-4 20 4.2341645421746076E-4 21 7.198079721696833E-4 22 0.004107139605909369 23 2.1170822710873038E-4 24 0.001651324171448097 25 7.621496175914294E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2361741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.4547569627336 #Duplication Level Percentage of deduplicated Percentage of total 1 87.20723463138907 64.92993459872159 2 9.399989041605368 13.997477990901736 3 1.8672470650036455 4.170762792426722 4 0.5860930373426427 1.7454965861158727 5 0.25064195785691956 0.9330743028450333 6 0.1567947121412003 0.7004467311308907 7 0.10221853552169415 0.5327459353548003 8 0.06450922956895762 0.3842415207527942 9 0.053007237495138526 0.3551976886469796 >10 0.2678950657161978 3.635390070224688 >50 0.021645916678787604 1.1118946174805402 >100 0.018345576724065792 2.7068004265767605 >500 0.0021313913077078603 1.0284971564734553 >1k 0.001958575796269222 2.466162906470073 >5k 2.8802585239253266E-4 1.3018766758781322 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6609 0.2798359345923198 No Hit CTGTAGGACGTGGAATATGGCAAGA 6268 0.26539743350350437 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6198 0.2624335183239822 No Hit GTATCAACGCAGAGTACTTTTTTTT 5791 0.24520046863733153 No Hit CTTTAGGACGTGAAATATGGCGAGG 5488 0.23237095007454245 No Hit GGTATCAACGCAGAGTACTTTTTTT 3873 0.16398919271842255 No Hit TATCAACGCAGAGTACTTTTTTTTT 3705 0.15687579628756923 No Hit GTCCTACAGTGTGCATTTCTCATTT 3151 0.13341852472392188 No Hit CTGAAGGACCTGGAATATGGCGAGA 2972 0.12583937019342933 No Hit ATTTAGAAATGTCCACTGTAGGACG 2540 0.10754777937123504 No Hit CTGTAGGACCTGGAATATGGCGAGA 2367 0.10022267471327295 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.234164542174608E-5 2 0.0 0.0 0.0 0.0 4.234164542174608E-5 3 0.0 0.0 0.0 0.0 4.234164542174608E-5 4 0.0 0.0 0.0 0.0 4.234164542174608E-5 5 0.0 0.0 0.0 0.0 4.234164542174608E-5 6 0.0 0.0 0.0 0.0 4.234164542174608E-5 7 0.0 0.0 0.0 0.0 4.234164542174608E-5 8 0.0 0.0 0.0 4.234164542174608E-5 4.234164542174608E-5 9 0.0 0.0 0.0 8.468329084349216E-5 4.234164542174608E-5 10 0.0 0.0 0.0 8.468329084349216E-5 4.234164542174608E-5 11 0.0 0.0 0.0 1.2702493626523824E-4 4.234164542174608E-5 12 0.0 0.0 0.0 1.2702493626523824E-4 4.234164542174608E-5 13 0.0 0.0 0.0 1.2702493626523824E-4 4.234164542174608E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 110 8.367351E-11 14.537796 1 GGTATCA 1215 0.0 14.066664 1 CCAACGA 105 2.1638698E-8 12.59333 19 GGCGAGG 1715 0.0 12.391396 19 TAGGACC 1185 0.0 12.274511 4 AGGACCG 80 3.0433615E-5 11.806247 5 CGTAGGA 85 3.1719173E-5 11.7585125 2 TCCAACG 190 0.0 11.433417 18 ATAGGAC 385 0.0 11.284932 3 ATTGCGA 60 0.0061366996 11.019163 17 CCACCTT 1060 0.0 10.692451 13 TATGTCG 230 0.0 10.676953 16 GTCAACG 75 0.0017366181 10.661051 2 CACCTTT 1065 0.0 10.64225 14 TGGCGAG 3580 0.0 10.605835 18 AGGACGT 4120 0.0 10.591235 5 CGTTAGG 85 4.1316223E-4 10.582662 1 TTAGGAC 2415 0.0 10.5205145 3 GACGTGA 2175 0.0 10.508916 7 GGACGTG 4055 0.0 10.458209 6 >>END_MODULE