FastQCFastQC Report
Thu 26 May 2016
SRR1511841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361741
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72590.30735800411645475No Hit
GTCCTACAGTGGACATTTCTAAATT71600.3031661812197019No Hit
GTCCTAAAGTGTGTATTTCTCATTT65730.278311635357137No Hit
CTGTAGGACGTGGAATATGGCAAGA63960.2708171641174879No Hit
CTTTAGGACGTGAAATATGGCGAGG60720.2570984710008422No Hit
GGTATCAACGCAGAGTACTTTTTTT48160.20391736435112912No Hit
TATCAACGCAGAGTACTTTTTTTTT48070.20353628954233338No Hit
GTCCTACAGTGTGCATTTCTCATTT34250.14502013556948032No Hit
CTGAAGGACCTGGAATATGGCGAGA33870.14341115304345398No Hit
TTTCTAAATTTTCCACCTTTTTCAG28090.11893768198968473No Hit
ATTTAGAAATGTCCACTGTAGGACG26760.11330624314859249No Hit
CTGTAGGACCTGGAATATGGCGAGA26260.11118916087750519No Hit
GTCCTTCAGTGTGCATTTCTCATTT25370.10742075443496979No Hit
GAATATGGCAAGAAAACTGAAAATC24330.10301722331110821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.012.7855741
GGGTGCG550.003076412212.0871518
TAGGACC12050.011.7472154
CTCGAAC2250.011.39766318
TCCAACG2000.010.92275918
TGTAGGA37850.010.8682032
GGCGAGG18150.010.78015819
TCGAACT2300.010.73692919
CCACCTT8950.010.71733813
CGCATTT803.779391E-410.68530819
CACCTTT9700.010.57403214
AGGACGT40750.010.561035
GGACGTG40100.010.5391176
TTAGGAC25300.010.5141273
CCAACGA1451.449007E-810.4806119
CTGTAGG37700.010.4492771
GACGTGA22500.010.4256477
GTCGAGG1659.331416E-1010.36151119
AGGACCT23650.010.3237545
GTATCAA30050.010.2526381
Produced by FastQC (version 0.11.5)