##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511841_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2361741 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3147165586743 33.0 31.0 34.0 28.0 34.0 2 31.510272294887542 33.0 31.0 34.0 28.0 34.0 3 31.630596242348336 34.0 31.0 34.0 28.0 34.0 4 35.179798716285994 37.0 35.0 37.0 32.0 37.0 5 34.875898754351134 37.0 35.0 37.0 32.0 37.0 6 34.834416220914996 37.0 35.0 37.0 32.0 37.0 7 34.6979435933068 37.0 35.0 37.0 32.0 37.0 8 34.62403963855478 37.0 35.0 37.0 32.0 37.0 9 36.20125449827056 39.0 37.0 39.0 32.0 39.0 10 36.034139221870646 39.0 37.0 39.0 31.0 39.0 11 36.01109435793341 39.0 37.0 39.0 31.0 39.0 12 35.88117452337068 39.0 37.0 39.0 30.0 39.0 13 35.873028414207994 39.0 37.0 39.0 30.0 39.0 14 36.99138940298703 40.0 37.0 41.0 31.0 41.0 15 36.95282039817236 40.0 37.0 41.0 31.0 41.0 16 36.87201941279759 40.0 37.0 41.0 31.0 41.0 17 36.83337969743507 40.0 37.0 41.0 31.0 41.0 18 36.77215748890331 40.0 37.0 41.0 30.0 41.0 19 36.79509692214345 40.0 37.0 41.0 30.0 41.0 20 36.683061351773965 40.0 37.0 41.0 30.0 41.0 21 36.580496337235964 40.0 37.0 41.0 30.0 41.0 22 36.67126793327465 40.0 37.0 41.0 30.0 41.0 23 36.58011145167908 40.0 37.0 41.0 30.0 41.0 24 36.49245069632953 40.0 37.0 41.0 30.0 41.0 25 36.10735470146811 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3082705811051021 1101 2 0.3138496681131855 1101 3 0.2999927603659813 1101 4 0.2988809402611139 1101 5 0.5419460539884895 1101 6 0.46726020523372824 1101 7 0.49850864850704824 1101 8 0.5107246353102326 1101 9 0.4756376508359281 1101 10 0.5930348875528964 1101 11 0.4158824926262099 1101 12 0.5818207148002017 1101 13 0.5067615918459225 1101 14 0.5224132626353466 1101 15 0.4226874976436079 1101 16 0.4694111054720196 1101 17 0.5693340898931396 1101 18 0.5549270806674471 1101 19 0.6113601640875146 1101 20 0.6218242009489998 1101 21 0.4475455621465372 1101 22 0.3934341631875711 1101 23 0.3102009648352322 1101 24 0.37082055995999497 1101 25 0.4498244551534114 1102 1 0.4358259273132994 1102 2 0.41578337661902154 1102 3 0.4163871377366348 1102 4 0.3817130794881223 1102 5 0.4838232387072381 1102 6 0.5451291592957546 1102 7 0.5252508454974034 1102 8 0.5038847612491963 1102 9 0.4085949852931279 1102 10 0.5017935954389543 1102 11 0.598196497138197 1102 12 0.5924288413341543 1102 13 0.7264110452014023 1102 14 0.8900905812262181 1102 15 0.941660153022049 1102 16 0.850773130411902 1102 17 1.0695423354245364 1102 18 0.8821923705997676 1102 19 0.9935105471105459 1102 20 0.9761688473034624 1102 21 0.9433856097455191 1102 22 0.6876082465353619 1102 23 0.9242277888429697 1102 24 0.9458930740289659 1102 25 1.0193685461864632 1103 1 0.31818639818704497 1103 2 0.3542759485017477 1103 3 0.33488158147115854 1103 4 0.3609296064928742 1103 5 0.36580648979818164 1103 6 0.350047570448659 1103 7 0.40790480695171993 1103 8 0.29264234044069326 1103 9 0.4093268486602213 1103 10 0.48573183572730017 1103 11 0.4356535667432482 1103 12 0.5133003615035818 1103 13 0.38426108532347314 1103 14 0.6118862789041799 1103 15 0.5351987134978771 1103 16 0.41557723147699477 1103 17 0.5113026476511706 1103 18 0.7021452148391205 1103 19 0.623896093784424 1103 20 0.6228697523459985 1103 21 0.5280683823744852 1103 22 0.5953936814680176 1103 23 0.6442274732145634 1103 24 0.6888100109341906 1103 25 0.6223272994717917 1104 1 0.3728580816153695 1104 2 0.35035102273662844 1104 3 0.37794000632305824 1104 4 0.36660094034704827 1104 5 0.40188701346151134 1104 6 0.4149444544328773 1104 7 0.5541208287310511 1104 8 0.5151825388558819 1104 9 0.47114150415416134 1104 10 0.5129894254877243 1104 11 0.5734012435796032 1104 12 0.7019086740132536 1104 13 0.7218663415145201 1104 14 0.8854007820523293 1104 15 0.7557856428515066 1104 16 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-0.9871987499302719 2314 24 -0.9961158368511249 2314 25 -1.0435624822010183 2315 1 -0.7076353132818056 2315 2 -0.697523894293024 2315 3 -0.7006213083693389 2315 4 -0.6803002789933927 2315 5 -0.7304682960407831 2315 6 -0.7862441925078443 2315 7 -0.8436006523630795 2315 8 -0.783577816254045 2315 9 -0.8499389008232328 2315 10 -0.9132171733627246 2315 11 -0.9763021788722526 2315 12 -0.9749188998674541 2315 13 -0.9438403637526989 2315 14 -1.0534452559280396 2315 15 -1.2414397845049905 2315 16 -1.2796383497733004 2315 17 -1.387029681060909 2315 18 -1.3124993488509205 2315 19 -1.381467176607913 2315 20 -1.2342858670864487 2315 21 -1.2673354262854133 2315 22 -1.4933790051379248 2315 23 -1.3634711325543876 2315 24 -1.1344779279167767 2315 25 -1.2159882353761304 2316 1 -1.5631859538883184 2316 2 -1.1082522847762917 2316 3 -0.9072486206915151 2316 4 -0.7968040572984094 2316 5 -0.8790263542486727 2316 6 -0.8726874722583062 2316 7 -0.9523267720405997 2316 8 -0.9166747603968943 2316 9 -1.1409628386109034 2316 10 -1.2301957655326632 2316 11 -1.0077170189403049 2316 12 -1.2651758077290438 2316 13 -1.1231774215251988 2316 14 -1.400378104319735 2316 15 -1.3548290241425178 2316 16 -1.3683958754787326 2316 17 -1.4266228225722202 2316 18 -1.4022051121996029 2316 19 -1.254283809788383 2316 20 -1.27952946238657 2316 21 -1.4455045630863168 2316 22 -1.3554564746639954 2316 23 -1.2730852196919642 2316 24 -1.1709003067223236 2316 25 -1.2937754955303404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26038.0 3 1.0 4 491.0 5 4361.0 6 5801.0 7 4612.0 8 3658.0 9 3274.0 10 3165.0 11 3005.0 12 2859.0 13 2871.0 14 2730.0 15 2794.0 16 2815.0 17 3011.0 18 3194.0 19 3635.0 20 4541.0 21 5782.0 22 7636.0 23 9350.0 24 11593.0 25 14183.0 26 16555.0 27 18917.0 28 22011.0 29 27226.0 30 34751.0 31 45653.0 32 59748.0 33 78656.0 34 105059.0 35 142421.0 36 211478.0 37 387266.0 38 1080600.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.054907488312466 20.361712938455227 13.584495708724779 24.99888386450753 2 13.917487141900828 21.199360979887295 42.707096163381166 22.17605571483071 3 18.000788401437752 25.972534668280726 30.773526817716252 25.253150112565265 4 11.915785854587782 18.111892879024413 39.711297724856365 30.26102354153144 5 12.137516962605185 38.72264171081321 36.900336965010865 12.239504361570742 6 29.810889509052856 38.82665372705982 19.011822210818206 12.350634553069114 7 26.577258048194107 32.17130921637894 23.68701733170572 17.564415403721238 8 23.453079740750574 38.844098484973586 21.46238728124718 16.240434493028662 9 25.988836201768102 15.178675392432956 21.43617780273112 37.396310603067825 10 14.35335203987228 29.619081855292347 35.896865913747526 20.130700191087847 11 32.86495005167798 22.358632889889282 26.631243646106835 18.145173412325907 12 23.003707857889584 25.097756273867454 33.31711648313681 18.58141938510616 13 28.635316065563497 22.8993780435704 26.389303484166977 22.076002406699125 14 19.543336885797384 23.65551514751194 28.8987657833776 27.902382183313073 15 21.537670726807047 33.475304870432446 23.822891671864106 21.1641327308964 16 20.37997408690203 28.953398765317097 30.03158688085903 20.635040266921845 17 20.364764806979256 29.920808420567706 29.18059177530474 20.53383499714829 18 20.022534242776192 27.857396604789948 32.372882863008996 19.74718628942487 19 22.724337681396904 27.22190959974019 28.78939731325323 21.264355405609674 20 22.719172000655448 28.193819728750952 29.67099271257941 19.416015558014195 21 21.734982794472383 27.34042386527566 28.377963544690125 22.546629795561834 22 22.04488129731414 29.608157710773536 28.624349579399265 19.722611412513057 23 20.691261234826342 29.1795332341692 29.397592708091192 20.731612822913267 24 22.185726589606634 27.492121939920956 30.277326346045218 20.044825124427195 25 21.5261720283232 28.37955421831117 29.650397440191316 20.443876313174318 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 480.0 3 707.0 4 707.0 5 707.0 6 1841.5 7 2976.0 8 2976.0 9 2976.0 10 4241.0 11 5506.0 12 5506.0 13 5506.0 14 8982.5 15 12459.0 16 12459.0 17 12459.0 18 23099.0 19 33739.0 20 33739.0 21 33739.0 22 54736.5 23 75734.0 24 75734.0 25 75734.0 26 109590.5 27 143447.0 28 143447.0 29 143447.0 30 183797.0 31 224147.0 32 224147.0 33 224147.0 34 248444.0 35 272741.0 36 272741.0 37 272741.0 38 283768.0 39 294795.0 40 294795.0 41 294795.0 42 300648.5 43 306502.0 44 306502.0 45 306502.0 46 304619.0 47 302736.0 48 302736.0 49 302736.0 50 281366.5 51 259997.0 52 259997.0 53 259997.0 54 220538.5 55 181080.0 56 181080.0 57 181080.0 58 149908.0 59 118736.0 60 118736.0 61 118736.0 62 93890.5 63 69045.0 64 69045.0 65 69045.0 66 51762.0 67 34479.0 68 34479.0 69 34479.0 70 24518.0 71 14557.0 72 14557.0 73 14557.0 74 10074.5 75 5592.0 76 5592.0 77 5592.0 78 3704.5 79 1817.0 80 1817.0 81 1817.0 82 1169.0 83 521.0 84 521.0 85 521.0 86 327.0 87 133.0 88 133.0 89 133.0 90 83.5 91 34.0 92 34.0 93 34.0 94 19.0 95 4.0 96 4.0 97 4.0 98 4.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4181660901851642 2 0.0 3 0.0 4 0.0 5 0.028157194205461138 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 4.234164542174608E-5 17 0.0 18 8.468329084349216E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005038655805187783 25 8.468329084349216E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2361741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.9492414551776 #Duplication Level Percentage of deduplicated Percentage of total 1 85.81542359553335 61.743546328533746 2 10.263648878484261 14.76923502738454 3 2.190382444277455 4.727890660875021 4 0.691694443427181 1.9906756209338774 5 0.2867985270499126 1.0317468235851726 6 0.1641029303588309 0.7084248815933831 7 0.10366697509096166 0.5221132155223238 8 0.07650242684436738 0.44034332647459606 9 0.051600355875590226 0.334134581765934 >10 0.3080345441070301 4.021939152216204 >50 0.023025929313500035 1.1361045303675492 >100 0.0202417811164884 3.006025988466479 >500 0.0023504426608189606 1.133138752032764 >1k 0.002232920527775714 3.0200544912279335 >5k 2.9380533260206764E-4 1.4146266190204337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7259 0.30735800411645475 No Hit GTCCTACAGTGGACATTTCTAAATT 7160 0.3031661812197019 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6573 0.278311635357137 No Hit CTGTAGGACGTGGAATATGGCAAGA 6396 0.2708171641174879 No Hit CTTTAGGACGTGAAATATGGCGAGG 6072 0.2570984710008422 No Hit GGTATCAACGCAGAGTACTTTTTTT 4816 0.20391736435112912 No Hit TATCAACGCAGAGTACTTTTTTTTT 4807 0.20353628954233338 No Hit GTCCTACAGTGTGCATTTCTCATTT 3425 0.14502013556948032 No Hit CTGAAGGACCTGGAATATGGCGAGA 3387 0.14341115304345398 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2809 0.11893768198968473 No Hit ATTTAGAAATGTCCACTGTAGGACG 2676 0.11330624314859249 No Hit CTGTAGGACCTGGAATATGGCGAGA 2626 0.11118916087750519 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2537 0.10742075443496979 No Hit GAATATGGCAAGAAAACTGAAAATC 2433 0.10301722331110821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.234164542174608E-5 6 0.0 0.0 0.0 0.0 4.234164542174608E-5 7 0.0 0.0 0.0 4.234164542174608E-5 4.234164542174608E-5 8 0.0 0.0 0.0 8.468329084349216E-5 4.234164542174608E-5 9 0.0 0.0 0.0 8.468329084349216E-5 4.234164542174608E-5 10 0.0 0.0 0.0 8.468329084349216E-5 4.234164542174608E-5 11 0.0 0.0 0.0 8.468329084349216E-5 4.234164542174608E-5 12 0.0 0.0 0.0 8.468329084349216E-5 8.468329084349216E-5 13 0.0 0.0 0.0 8.468329084349216E-5 8.468329084349216E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1410 0.0 12.785574 1 GGGTGCG 55 0.0030764122 12.087151 8 TAGGACC 1205 0.0 11.747215 4 CTCGAAC 225 0.0 11.397663 18 TCCAACG 200 0.0 10.922759 18 TGTAGGA 3785 0.0 10.868203 2 GGCGAGG 1815 0.0 10.780158 19 TCGAACT 230 0.0 10.736929 19 CCACCTT 895 0.0 10.717338 13 CGCATTT 80 3.779391E-4 10.685308 19 CACCTTT 970 0.0 10.574032 14 AGGACGT 4075 0.0 10.56103 5 GGACGTG 4010 0.0 10.539117 6 TTAGGAC 2530 0.0 10.514127 3 CCAACGA 145 1.449007E-8 10.48061 19 CTGTAGG 3770 0.0 10.449277 1 GACGTGA 2250 0.0 10.425647 7 GTCGAGG 165 9.331416E-10 10.361511 19 AGGACCT 2365 0.0 10.323754 5 GTATCAA 3005 0.0 10.252638 1 >>END_MODULE