FastQCFastQC Report
Thu 26 May 2016
SRR1511840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472543
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58100.23498074654313394No Hit
GTATCAACGCAGAGTACTTTTTTTT57680.23328209054402693No Hit
CTGTAGGACGTGGAATATGGCAAGA52790.21350488141156695No Hit
GTCCTAAAGTGTGTATTTCTCATTT50220.20311072446465034No Hit
CTTTAGGACGTGAAATATGGCGAGG45580.1843446200935636No Hit
GGTATCAACGCAGAGTACTTTTTTT37730.1525959305864448No Hit
TATCAACGCAGAGTACTTTTTTTTT35020.14163555497315922No Hit
GTCCTACAGTGTGCATTTCTCATTT28430.11498283346336141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1750.012.95380918
GCGTAAG550.002101327812.7172371
TAGGACC10700.012.53513054
GGTATCA11300.012.4680391
TATGTCG1750.011.87432516
ACAGCGC1302.8685463E-911.6242738
GCCGGTT1002.069768E-611.33435311
ATGTCGA1950.011.1408317
GTATCAA25150.011.0846931
GTCCTAT3250.011.0681911
GGTGCGC600.00613816611.0188438
GCGCCAC1409.147698E-910.79462213
TTAGGAC19800.010.7812113
AGGACCT22800.010.7711685
CCAACGA1159.5010546E-710.67750619
GGCGAGG13950.010.630403519
GTCCTAC29500.010.6018041
AGGACGT33000.010.561775
GGACGTG32000.010.5081036
CGGTAGG1155.626762E-610.4265561
Produced by FastQC (version 0.11.5)