##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2472543 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.35966411908711 31.0 30.0 34.0 25.0 34.0 2 26.628964592324582 31.0 26.0 34.0 10.0 34.0 3 28.363527348159366 31.0 28.0 34.0 16.0 34.0 4 32.72282666064857 35.0 33.0 37.0 26.0 37.0 5 33.09082794515606 35.0 35.0 37.0 27.0 37.0 6 33.29324302954488 37.0 35.0 37.0 27.0 37.0 7 33.27470139043082 37.0 35.0 37.0 27.0 37.0 8 33.290359763207356 37.0 35.0 37.0 27.0 37.0 9 34.82548170041937 39.0 35.0 39.0 27.0 39.0 10 34.69616382809116 39.0 35.0 39.0 27.0 39.0 11 34.69588233652559 39.0 35.0 39.0 27.0 39.0 12 34.57766679891917 39.0 35.0 39.0 27.0 39.0 13 34.570938098953185 39.0 35.0 39.0 27.0 39.0 14 35.64649634000298 40.0 36.0 41.0 27.0 41.0 15 35.59293043639686 40.0 36.0 41.0 27.0 41.0 16 35.53935523062693 40.0 36.0 41.0 27.0 41.0 17 35.49377179689089 40.0 36.0 41.0 27.0 41.0 18 35.46155638142592 40.0 36.0 41.0 26.0 41.0 19 35.483886023417995 40.0 36.0 41.0 26.0 41.0 20 35.423542077933526 40.0 36.0 41.0 26.0 41.0 21 35.311137561611666 40.0 36.0 41.0 25.0 41.0 22 35.21511334686596 40.0 36.0 41.0 25.0 41.0 23 35.09485618652537 40.0 36.0 41.0 25.0 41.0 24 34.98359381414196 40.0 35.0 41.0 24.0 41.0 25 34.87115128028107 39.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7950533601959329 1101 2 3.2087880999722884 1101 3 1.880075962993942 1101 4 1.121799802810365 1101 5 0.4244827893878025 1101 6 0.5360377877910807 1101 7 0.2741766584753407 1101 8 0.2813837595860065 1101 9 0.4421092533768558 1101 10 0.6448399732858476 1101 11 0.4155548523713293 1101 12 0.3633515482104954 1101 13 0.32007654154164555 1101 14 0.43361159669069593 1101 15 0.523842867683193 1101 16 0.49628796571987266 1101 17 0.6285933102803369 1101 18 0.6196122018745029 1101 19 0.6783512916044572 1101 20 0.5209093405887728 1101 21 0.6026873742027092 1101 22 0.6032192146835555 1101 23 0.39865650220355775 1101 24 0.5558233597247266 1101 25 0.595298075578043 1102 1 1.3750723952874324 1102 2 4.119720562672455 1102 3 2.403081461468151 1102 4 1.5402920463004932 1102 5 1.2545585652872902 1102 6 1.1698779642591361 1102 7 0.8890024517698194 1102 8 0.9283631606018758 1102 9 0.9112364555000383 1102 10 1.0346377275095193 1102 11 0.9022472691487167 1102 12 1.0392252738202288 1102 13 1.0661282924718662 1102 14 1.2044733694557692 1102 15 1.178376458241523 1102 16 1.2447427105767588 1102 17 1.254059661738701 1102 18 1.1581387963326222 1102 19 1.331375038253853 1102 20 1.2267034882507204 1102 21 1.337206452367525 1102 22 1.2397578928629471 1102 23 1.2651443455889861 1102 24 1.0771038548027718 1102 25 1.1074128593334436 1103 1 1.279728666312483 1103 2 3.892206947374831 1103 3 2.310731090947929 1103 4 1.6897321071382265 1103 5 1.2209156845936633 1103 6 1.1281804350529754 1103 7 1.069095629512688 1103 8 0.8936281004840794 1103 9 0.9080719509918254 1103 10 1.0757754873015344 1103 11 1.1593397952584752 1103 12 1.08250265883634 1103 13 1.1401666343801438 1103 14 1.3908522205165639 1103 15 1.2087581778459722 1103 16 1.242218855472963 1103 17 1.2421233728042225 1103 18 1.422967842993188 1103 19 1.455038746044231 1103 20 1.4346806916762773 1103 21 1.3717466016939497 1103 22 1.4103172169378837 1103 23 1.3880453850266719 1103 24 1.544058986777948 1103 25 1.6151876231389082 1104 1 1.2851208689890967 1104 2 3.8428846104421375 1104 3 2.2090702332560355 1104 4 1.5192861875915753 1104 5 1.2157161012897717 1104 6 1.0382904446497463 1104 7 0.9543537508697284 1104 8 1.0553143976102888 1104 9 1.1011772412240148 1104 10 1.0527859662513137 1104 11 1.038837191667625 1104 12 1.1783059120141814 1104 13 1.188048315176026 1104 14 1.2832682221335077 1104 15 1.210695199289411 1104 16 1.3188220983169785 1104 17 1.1011123148565645 1104 18 1.227846887905038 1104 19 1.2513534473474337 1104 20 1.2344122023060535 1104 21 1.3538274911927388 1104 22 1.2399182174948464 1104 23 1.3075204193524286 1104 24 1.2261105084709882 1104 25 1.2608821432507256 1105 1 1.3143912479892705 1105 2 4.029240099269348 1105 3 2.39532919368979 1105 4 1.5579268747549335 1105 5 1.154375277353111 1105 6 0.9240190186877726 1105 7 0.8667586889653265 1105 8 0.7716367549227598 1105 9 1.031622593530372 1105 10 0.8829810130081839 1105 11 0.9116298471934314 1105 12 0.6898299171405782 1105 13 0.8526321961223644 1105 14 0.9418558335309797 1105 15 1.0663514661354725 1105 16 1.0495724803466615 1105 17 0.9219927429080101 1105 18 1.1699963079178985 1105 19 1.0729778865545896 1105 20 1.151326130165188 1105 21 1.1924435264894626 1105 22 1.1345054992107393 1105 23 1.2234022359276935 1105 24 1.2812608922827309 1105 25 1.338294573434709 1106 1 0.9021667847981725 1106 2 3.6430883623423647 1106 3 1.9435035084882415 1106 4 1.1476630171801716 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2314 4 0.35989735833354786 2314 5 0.07461264907905729 2314 6 -0.07214481703859121 2314 7 -0.21564649874781594 2314 8 -0.2640997856589209 2314 9 -0.277784397313134 2314 10 -0.3546513886584819 2314 11 -0.28469092656075645 2314 12 -0.5094142120338532 2314 13 -0.5141181274291711 2314 14 -1.1514832360490175 2314 15 -0.7438360724406863 2314 16 -0.6144890071678333 2314 17 -0.7231462318700608 2314 18 -0.48724825798453253 2314 19 -0.6148515457167676 2314 20 -0.6115045300337982 2314 21 -0.5255142197810443 2314 22 -0.3083298833316235 2314 23 -0.500419974353882 2314 24 -0.3212027151970318 2314 25 -0.4612040770284409 2315 1 -4.274679853834307 2315 2 -13.518187114934165 2315 3 -6.8682485030504665 2315 4 -3.145551897506209 2315 5 -1.993800780695338 2315 6 -1.351755272085704 2315 7 -1.1829229583686711 2315 8 -1.1293938639063086 2315 9 -1.2356603975997231 2315 10 -1.371177456846354 2315 11 -1.1507440662278228 2315 12 -1.1664732965346047 2315 13 -1.2285880435088359 2315 14 -1.4237260694240064 2315 15 -1.454772764734578 2315 16 -1.4719825478527042 2315 17 -1.3874646507258674 2315 18 -1.3016805535566363 2315 19 -1.366879307410919 2315 20 -1.3160945120100322 2315 21 -1.5353204064875143 2315 22 -1.4818606589838765 2315 23 -1.5008580245567202 2315 24 -1.5888061138318221 2315 25 -1.5243735067720294 2316 1 -1.4644855154814174 2316 2 -1.2822230014698128 2316 3 -0.8494182831107402 2316 4 -1.0196799747857028 2316 5 -1.14153947101034 2316 6 -1.4364813293448222 2316 7 -1.3887742633732039 2316 8 -1.4641107728381044 2316 9 -1.4253667345502166 2316 10 -1.6297659936611382 2316 11 -1.5138939764696673 2316 12 -1.5041772650267191 2316 13 -1.5327899660864546 2316 14 -1.9015726542401552 2316 15 -2.3772576804497305 2316 16 -2.2249459960158475 2316 17 -1.9656629843711144 2316 18 -1.9204556395770496 2316 19 -1.800192880357244 2316 20 -2.1498528504925645 2316 21 -2.1096736545967687 2316 22 -2.0923722787536008 2316 23 -1.9791980962865807 2316 24 -1.9633476955061724 2316 25 -2.169026335591667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97809.0 3 4.0 4 907.0 5 4423.0 6 5403.0 7 4840.0 8 4331.0 9 4243.0 10 3981.0 11 3851.0 12 3780.0 13 3916.0 14 3870.0 15 4010.0 16 4255.0 17 4594.0 18 5104.0 19 6222.0 20 8069.0 21 10635.0 22 12901.0 23 15221.0 24 18256.0 25 20440.0 26 22838.0 27 25471.0 28 29565.0 29 35694.0 30 44398.0 31 56540.0 32 72223.0 33 94252.0 34 127862.0 35 180519.0 36 279279.0 37 374126.0 38 878711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51820898564757 19.400956828657783 12.885235969607 25.19559821608765 2 15.68431987294894 21.270228226419228 40.89776873421274 22.147683166419093 3 18.17711562549165 25.46855605746796 30.11256022645511 26.241768090585282 4 11.813262701599122 17.2608929349257 39.62483160050199 31.30101276297318 5 12.458286670622128 38.45792024447823 36.5165896807765 12.567203404123143 6 30.50622544251501 38.17667435789065 18.778531171701687 12.53856902789265 7 27.049843015874746 31.974448978238197 23.269726755004868 17.70598125088219 8 24.017539836516494 38.15189462832396 21.21313967037176 16.61742586478779 9 26.172527636526443 15.27386985787507 21.014194697523966 37.53940780807452 10 14.4444404000254 29.347113477905136 35.6542232025894 20.55422291948007 11 33.34756968837347 22.420439199641827 26.023409906319124 18.208581205665585 12 23.078344845772147 24.79301674429929 32.915302180791194 19.213336229137372 13 28.602351106433954 22.622341901317593 26.33581364573568 22.439493346512773 14 20.078720572301474 23.069568456443427 28.55735977089175 28.294351200363348 15 22.063680995638904 32.47563338635567 23.73572471742655 21.72496090057888 16 21.032111473895498 28.660249791409086 29.27075484632623 21.036883888369182 17 20.85604126314802 29.449861093191927 28.726550755281753 20.9675468883783 18 20.488218000657625 27.79984008367094 31.405763216251447 20.30617869941999 19 22.882827353832973 27.271447759401195 28.364902199406195 21.480822687359634 20 22.949693854976356 27.85974315283874 29.12695180344272 20.06361118874218 21 22.089213470149268 27.20921457024193 28.01762089593649 22.68395106367231 22 22.472990331849243 29.01504905259888 28.14712382221992 20.364836793331953 23 21.209132007891498 28.72368994723632 28.818612807751077 21.24856523712111 24 22.45738547890932 27.25832897205031 29.62498897882884 20.659296570211534 25 21.912368161143707 28.08160561304512 29.084102654532245 20.921923571278928 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 243.0 1 243.0 2 434.5 3 626.0 4 626.0 5 626.0 6 1707.0 7 2788.0 8 2788.0 9 2788.0 10 4156.5 11 5525.0 12 5525.0 13 5525.0 14 9338.5 15 13152.0 16 13152.0 17 13152.0 18 23321.0 19 33490.0 20 33490.0 21 33490.0 22 53269.5 23 73049.0 24 73049.0 25 73049.0 26 105305.0 27 137561.0 28 137561.0 29 137561.0 30 176637.5 31 215714.0 32 215714.0 33 215714.0 34 242907.0 35 270100.0 36 270100.0 37 270100.0 38 284070.0 39 298040.0 40 298040.0 41 298040.0 42 307361.0 43 316682.0 44 316682.0 45 316682.0 46 317962.5 47 319243.0 48 319243.0 49 319243.0 50 300489.0 51 281735.0 52 281735.0 53 281735.0 54 244016.5 55 206298.0 56 206298.0 57 206298.0 58 172825.5 59 139353.0 60 139353.0 61 139353.0 62 111557.0 63 83761.0 64 83761.0 65 83761.0 66 63830.0 67 43899.0 68 43899.0 69 43899.0 70 31739.5 71 19580.0 72 19580.0 73 19580.0 74 13659.0 75 7738.0 76 7738.0 77 7738.0 78 5231.5 79 2725.0 80 2725.0 81 2725.0 82 1798.0 83 871.0 84 871.0 85 871.0 86 570.0 87 269.0 88 269.0 89 269.0 90 170.5 91 72.0 92 72.0 93 72.0 94 50.0 95 28.0 96 28.0 97 28.0 98 14.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.468661212363142 3 0.0 4 0.0 5 4.853302854591406E-4 6 0.001132437332737995 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0222095227464192E-4 14 0.0 15 0.0 16 0.0 17 8.897721900084246E-4 18 0.0 19 4.853302854591406E-4 20 8.897721900084246E-4 21 9.706605709182812E-4 22 0.003842198093218197 23 3.639977140943555E-4 24 0.0011728815231929232 25 6.875512377337825E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2472543.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.99571965064386 #Duplication Level Percentage of deduplicated Percentage of total 1 85.50669525012789 63.27129449981504 2 10.31071884510465 15.258981221179482 3 2.3314289997199382 5.1754729994597275 4 0.7792969044593115 2.3065854106794323 5 0.32719456893351023 1.2105498797008645 6 0.1848881297031922 0.8208558127349573 7 0.11885386099369866 0.6156273884240425 8 0.07961064302520718 0.47126774580005737 9 0.05315985413064496 0.35402414966282936 >10 0.2714022682899661 3.574088905694106 >50 0.018148947584490913 0.9517245179806461 >100 0.015175137213009967 2.2691272686219386 >500 0.0017685629494326861 0.9179029216956135 >1k 0.0014369573963866644 1.9077412962166584 >5k 2.2107036867487144E-4 0.8947559823345467 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5810 0.23498074654313394 No Hit GTATCAACGCAGAGTACTTTTTTTT 5768 0.23328209054402693 No Hit CTGTAGGACGTGGAATATGGCAAGA 5279 0.21350488141156695 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5022 0.20311072446465034 No Hit CTTTAGGACGTGAAATATGGCGAGG 4558 0.1843446200935636 No Hit GGTATCAACGCAGAGTACTTTTTTT 3773 0.1525959305864448 No Hit TATCAACGCAGAGTACTTTTTTTTT 3502 0.14163555497315922 No Hit GTCCTACAGTGTGCATTTCTCATTT 2843 0.11498283346336141 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.0444190454928386E-5 0.0 10 0.0 0.0 0.0 4.0444190454928386E-5 0.0 11 0.0 0.0 0.0 4.0444190454928386E-5 0.0 12 0.0 0.0 0.0 8.088838090985677E-5 4.0444190454928386E-5 13 0.0 0.0 0.0 8.088838090985677E-5 4.0444190454928386E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 175 0.0 12.953809 18 GCGTAAG 55 0.0021013278 12.717237 1 TAGGACC 1070 0.0 12.5351305 4 GGTATCA 1130 0.0 12.468039 1 TATGTCG 175 0.0 11.874325 16 ACAGCGC 130 2.8685463E-9 11.624273 8 GCCGGTT 100 2.069768E-6 11.334353 11 ATGTCGA 195 0.0 11.14083 17 GTATCAA 2515 0.0 11.084693 1 GTCCTAT 325 0.0 11.068191 1 GGTGCGC 60 0.006138166 11.018843 8 GCGCCAC 140 9.147698E-9 10.794622 13 TTAGGAC 1980 0.0 10.781211 3 AGGACCT 2280 0.0 10.771168 5 CCAACGA 115 9.5010546E-7 10.677506 19 GGCGAGG 1395 0.0 10.6304035 19 GTCCTAC 2950 0.0 10.601804 1 AGGACGT 3300 0.0 10.56177 5 GGACGTG 3200 0.0 10.508103 6 CGGTAGG 115 5.626762E-6 10.426556 1 >>END_MODULE