FastQCFastQC Report
Thu 26 May 2016
SRR1511840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472543
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74640.3018754375555855No Hit
GTCCTACAGTGGACATTTCTAAATT63170.25548595110378264No Hit
CTGTAGGACGTGGAATATGGCAAGA56930.2302487762599073No Hit
GTCCTAAAGTGTGTATTTCTCATTT54220.21928840064662172No Hit
CTTTAGGACGTGAAATATGGCGAGG51180.20699336674832347No Hit
GGTATCAACGCAGAGTACTTTTTTT50350.20363649894056443No Hit
TATCAACGCAGAGTACTTTTTTTTT49550.20040096370417015No Hit
GTCCTACAGTGTGCATTTCTCATTT31560.127641865075754No Hit
CTGAAGGACCTGGAATATGGCGAGA29030.1174094848906571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.014.529911
AGTACGT604.094135E-412.66645055
TAGGACC9650.012.60082154
GGCGAAA701.09363966E-412.21160819
AATACCG658.0275093E-411.6921095
CCACCTT8900.011.5246313
GGCGAGG14100.011.31666719
CTAATAC2300.011.1519833
TCCACCT10150.010.85388512
ACTCCGT700.001495682910.8538846
TTCCACC9650.010.82576611
ACCTTTT9850.010.79878815
CACCTTT10300.010.78802314
GCGCCAC1158.856805E-710.73590813
TGTAGGA33400.010.6091012
TGGACTG3900.010.474185
GTATCAA31950.010.3602611
CTGTAGG33000.010.3485891
TGCACCG856.596913E-410.0586525
GTAGGAC33200.010.0435043
Produced by FastQC (version 0.11.5)