FastQCFastQC Report
Thu 26 May 2016
SRR1511839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320847
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61880.2666267961653655No Hit
GTCCTACAGTGGACATTTCTAAATT41920.18062371194654364No Hit
GGTATCAACGCAGAGTACTTTTTTT40550.17472069464294718No Hit
GTCCTAAAGTGTGTATTTCTCATTT39040.16821444929372767No Hit
CTGTAGGACGTGGAATATGGCAAGA38230.1647243441726232No Hit
TATCAACGCAGAGTACTTTTTTTTT37620.16209599340240868No Hit
CTTTAGGACGTGAAATATGGCGAGG34170.1472307308495562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.013.869351
ACTGCGC701.1519829E-412.1437228
CGGTAGG1001.0506446E-611.9902771
TAGGACC8650.010.9194444
GTCCTAT2600.010.7605061
TCCAACG2150.010.54406618
GGCGAGG11150.010.50466419
ATAGGAC2700.010.1449733
AGGACGT24550.010.0801375
CACCTTT7450.010.01562714
GTATCAA26100.09.9536141
AGGACCT16150.09.9424415
GGACGTG24550.09.8106096
GATATAC4800.09.7837331
CTAGACT1554.4450644E-89.7500084
GTCCTAA13250.09.652551
TTCCACC7350.09.63787411
GGACCTG15150.09.6009766
TTAGGAC15750.09.5952443
CTAGGAC3650.09.5747073
Produced by FastQC (version 0.11.5)