##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320847 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.361554208442005 31.0 30.0 34.0 25.0 34.0 2 26.633726393855348 31.0 26.0 34.0 10.0 34.0 3 28.365638924065223 31.0 28.0 34.0 16.0 34.0 4 32.73176947898763 35.0 33.0 37.0 26.0 37.0 5 33.09132872610732 35.0 35.0 37.0 27.0 37.0 6 33.28897251736112 37.0 35.0 37.0 27.0 37.0 7 33.267983628390844 37.0 35.0 37.0 27.0 37.0 8 33.28114218645176 37.0 35.0 37.0 27.0 37.0 9 34.81859381510285 39.0 35.0 39.0 27.0 39.0 10 34.68833921408865 39.0 35.0 39.0 27.0 39.0 11 34.69007220208829 39.0 35.0 39.0 27.0 39.0 12 34.567738416190295 39.0 35.0 39.0 27.0 39.0 13 34.55801265658615 39.0 35.0 39.0 27.0 39.0 14 35.638426832962274 40.0 36.0 41.0 27.0 41.0 15 35.57913684098952 40.0 36.0 41.0 27.0 41.0 16 35.5273441118695 40.0 36.0 41.0 27.0 41.0 17 35.48328907506613 40.0 36.0 41.0 26.0 41.0 18 35.44419084928907 40.0 36.0 41.0 26.0 41.0 19 35.467940368322424 40.0 36.0 41.0 26.0 41.0 20 35.4028486151823 40.0 36.0 41.0 25.0 41.0 21 35.296914014581745 40.0 36.0 41.0 25.0 41.0 22 35.1921759598974 40.0 36.0 41.0 25.0 41.0 23 35.07781340174514 40.0 36.0 41.0 24.0 41.0 24 34.961602811387394 40.0 35.0 41.0 24.0 41.0 25 34.85125689026463 39.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5992291875772686 1101 2 3.0217324347030043 1101 3 1.6857630328659923 1101 4 0.7624921068766213 1101 5 0.1427279946154556 1101 6 0.13938526669853957 1101 7 -0.04536351621701584 1101 8 0.02336027720527767 1101 9 0.05312016790612972 1101 10 0.017374479775440932 1101 11 -0.038425226164022774 1101 12 0.025656421215416003 1101 13 -0.100913269540861 1101 14 -0.0536730024655796 1101 15 0.18049924866653555 1101 16 0.15378967796648624 1101 17 0.21817995000286317 1101 18 0.28770690456542525 1101 19 0.30977410378756787 1101 20 0.09864516291931125 1101 21 0.1872299694616757 1101 22 0.20267198377921858 1101 23 0.02314851509917304 1101 24 0.2741120850905503 1101 25 0.23857177670734586 1102 1 1.5243785566324632 1102 2 4.106856681659842 1102 3 2.5203191402629628 1102 4 1.5652621487743303 1102 5 1.1357632234000903 1102 6 1.127810920988722 1102 7 1.146377593948344 1102 8 1.0732144202855451 1102 9 1.1552018312534855 1102 10 1.125343308540245 1102 11 1.1324066954285854 1102 12 1.1989212197014822 1102 13 1.34967470325455 1102 14 1.547573185834466 1102 15 1.4861674221081813 1102 16 1.4148314186634607 1102 17 1.3865856057805317 1102 18 1.3605642953380084 1102 19 1.4429030446101478 1102 20 1.5691919817509685 1102 21 1.566778598076077 1102 22 1.6328366822727176 1102 23 1.55645430227203 1102 24 1.290740600324952 1102 25 1.4266553076843778 1103 1 1.3796637360450248 1103 2 3.9570634417663584 1103 3 2.474746797230978 1103 4 1.6209545219432542 1103 5 1.224959360810729 1103 6 1.1224644899729768 1103 7 1.161458716376778 1103 8 1.0012780069263698 1103 9 0.9519707372940971 1103 10 1.1928615546164067 1103 11 1.264440996326364 1103 12 1.1532016427688916 1103 13 1.246798466000925 1103 14 1.5642083160732057 1103 15 1.1256318587947263 1103 16 1.1959601735592713 1103 17 1.3438981269885417 1103 18 1.4418408592668541 1103 19 1.403537530700568 1103 20 1.4444434584034695 1103 21 1.4728583229327228 1103 22 1.4777878822865205 1103 23 1.4704144916056237 1103 24 1.4465669793487237 1103 25 1.428568198127401 1104 1 1.1319541698789877 1104 2 3.5991246692276597 1104 3 1.981978522331108 1104 4 1.3270659674841951 1104 5 1.0758215740311172 1104 6 0.9008008259308014 1104 7 0.7807581667769767 1104 8 0.7796695419158155 1104 9 0.8233402600397923 1104 10 1.0006776505670985 1104 11 0.7888087609611745 1104 12 1.0001334241546473 1104 13 0.9751768531895308 1104 14 1.0360344260844414 1104 15 1.043838846470564 1104 16 1.0647788056229572 1104 17 0.9357425787252467 1104 18 0.9298066010962032 1104 19 0.9458354185769906 1104 20 0.9041179579720904 1104 21 0.9478060033781404 1104 22 0.8427897403641609 1104 23 0.9123238130704365 1104 24 0.8953581422880035 1104 25 0.8432635277242966 1105 1 1.2132065860965149 1105 2 3.8689691004594877 1105 3 2.2463871454917417 1105 4 1.416719482939179 1105 5 1.1046870398778523 1105 6 0.9283065011592981 1105 7 0.831382798316902 1105 8 0.8133397690004287 1105 9 0.8645193154905755 1105 10 0.7426981577023284 1105 11 0.7898318535015676 1105 12 0.6502382911400417 1105 13 0.7810693630619525 1105 14 0.808743935729602 1105 15 0.8586066841309403 1105 16 0.8877724750282141 1105 17 0.9522789408597561 1105 18 0.9059148744765935 1105 19 0.8780068989499696 1105 20 0.955263360387093 1105 21 1.046978472461042 1105 22 0.9612487617342111 1105 23 0.9688475794262956 1105 24 0.9818920640952271 1105 25 1.18247698759685 1106 1 1.139936422023201 1106 2 3.8422145238986367 1106 3 2.1474729945793527 1106 4 1.311013525832685 1106 5 1.0443513888979084 1106 6 0.731116343367006 1106 7 0.8253802274820004 1106 8 0.6351702290118979 1106 9 0.6589702925173881 1106 10 0.7065786577005042 1106 11 0.7038193498954612 1106 12 0.6742033840224053 1106 13 0.6048366221648678 1106 14 0.7346503011022065 1106 15 0.830513283215808 1106 16 0.8204373243385774 1106 17 0.784180938734437 1106 18 0.8302733525715453 1106 19 0.6449980544014906 1106 20 0.8862730521425277 1106 21 0.9712360708484269 1106 22 0.8882342204493057 1106 23 0.844545520932499 1106 24 0.9460838761321355 1106 25 0.948735509878027 1107 1 0.9191174706571736 1107 2 3.338297009102586 1107 3 1.836940957915317 1107 4 1.206262596365697 1107 5 0.8497544980648044 1107 6 0.765385946469074 1107 7 0.6650216617449516 1107 8 0.524921469040649 1107 9 0.49635704838922123 1107 10 0.6436357744451087 1107 11 0.6146761454304723 1107 12 0.6253895081228862 1107 13 0.5434407801431931 1107 14 0.5856917921535967 1107 15 0.5406560085283658 1107 16 0.3833134368151718 1107 17 0.4481191724365985 1107 18 0.31129581062352685 1107 19 0.5420612492834351 1107 20 0.49895572900744867 1107 21 0.40679650173068893 1107 22 0.4944403536004458 1107 23 0.659789666843281 1107 24 0.6622619256268081 1107 25 0.666575064798657 1108 1 1.0227441740658776 1108 2 3.614241669553305 1108 3 2.004701706848916 1108 4 1.3003105749244526 1108 5 0.9686930054824145 1108 6 0.7307770226836325 1108 7 0.6895979329313917 1108 8 0.7447640570894194 1108 9 0.6864366980815717 1108 10 0.6424740591455702 1108 11 0.7137626276319722 1108 12 0.8124200090477274 1108 13 0.6226771245620526 1108 14 0.7835901349870085 1108 15 0.765525775619075 1108 16 0.660291467089607 1108 17 0.8037163563858627 1108 18 0.8068112687036262 1108 19 0.8420555869830295 1108 20 0.7148941609277273 1108 21 0.6149020847588886 1108 22 0.6433088372363969 1108 23 0.7565753676473292 1108 24 0.7826292201796718 1108 25 0.6082533706383728 1109 1 0.5099704643360177 1109 2 2.569337724175096 1109 3 1.3932380577628152 1109 4 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-1.6889679448296562 2315 16 -1.735023192711182 2315 17 -1.6411957832889215 2315 18 -1.6093601330868026 2315 19 -1.7140151402821502 2315 20 -1.593703959656267 2315 21 -1.691640424569968 2315 22 -1.7239287831191987 2315 23 -1.8343238807049147 2315 24 -1.8754870330906144 2315 25 -1.934378167718208 2316 1 -1.130688578849579 2316 2 -1.1268625502094203 2316 3 -0.6988595162466034 2316 4 -0.7213206844694611 2316 5 -0.9964379598410318 2316 6 -1.0384381062024062 2316 7 -1.055973781647971 2316 8 -1.1986380709114357 2316 9 -1.2207142640338375 2316 10 -1.3971661946461538 2316 11 -1.5000978938529954 2316 12 -1.1788643660079643 2316 13 -1.2192784143592093 2316 14 -1.5286154232419165 2316 15 -1.9809790928809292 2316 16 -1.7285132107101546 2316 17 -1.5501426501906224 2316 18 -1.5975439446608348 2316 19 -1.6245097887439783 2316 20 -1.8553205105153623 2316 21 -1.7221919346200636 2316 22 -1.899810062286555 2316 23 -1.7665537203777646 2316 24 -1.6126992179199746 2316 25 -1.71704441284259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92122.0 3 1.0 4 790.0 5 4089.0 6 5098.0 7 4427.0 8 4117.0 9 3948.0 10 3869.0 11 3652.0 12 3684.0 13 3467.0 14 3712.0 15 3821.0 16 4088.0 17 4333.0 18 4856.0 19 5980.0 20 7758.0 21 9950.0 22 12277.0 23 14588.0 24 16815.0 25 19355.0 26 21456.0 27 24335.0 28 28265.0 29 33889.0 30 41522.0 31 53275.0 32 67716.0 33 87931.0 34 118661.0 35 168759.0 36 260769.0 37 349573.0 38 827899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.156140839960585 19.03249115516878 12.837856179231116 24.97351182563952 2 16.151550635464492 21.2988973552662 40.03998346329175 22.509568545977565 3 18.503417071439866 25.12358634584701 29.864312468680616 26.508684114032505 4 12.17805396047219 17.218886036003234 38.823886279448836 31.779173724075736 5 12.98384629636811 38.00058599725099 36.05597997268219 12.959587733698719 6 30.951821832169017 37.65802045222751 18.474653172602995 12.915504543000477 7 27.426366322295266 31.80313049503048 22.81602363275132 17.95447954992294 8 24.656256961359365 37.156520873629326 21.035768406965218 17.15145375804609 9 26.429014924292726 15.125857068561608 20.662154808136858 37.782973199008815 10 15.025764300705735 29.033236572682302 34.87093289648133 21.07006623013064 11 33.735485363748666 22.33163151211605 25.435498333151646 18.497384790983638 12 23.293392455426833 24.75372137844502 32.20470802254522 19.748178143582926 13 28.920956738564392 22.238557282849378 26.030541488588415 22.809944489997815 14 20.716876209418373 22.647076692259336 28.064323068259135 28.571724030063162 15 22.73872426747649 31.489107209566164 23.59668689922257 22.175481623734782 16 21.754385360172385 28.25899337612518 28.4135490189573 21.573072244745127 17 21.44462172783949 28.809231528906267 28.181610967460337 21.564535775793907 18 21.13780012211059 27.57769038631155 30.49537517983736 20.789134311740497 19 23.289429575747338 26.929888135041107 27.980544924798583 21.800137364412965 20 23.38035245164551 27.450277465509092 28.59667816974021 20.57269191310519 21 22.863540825550903 26.79795900252755 27.561092473110865 22.77740769881068 22 22.994496178176806 28.199535409011993 27.793589230382754 21.012379182428447 23 22.037566619284334 27.85917322069581 28.295696768019468 21.807563392000386 24 22.895582853648982 26.883006139701145 28.901924640543747 21.319486366106126 25 22.481593813918597 27.52842418400714 28.509541658282426 21.480440343791834 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 364.0 1 364.0 2 527.5 3 691.0 4 691.0 5 691.0 6 1686.5 7 2682.0 8 2682.0 9 2682.0 10 3912.0 11 5142.0 12 5142.0 13 5142.0 14 8155.5 15 11169.0 16 11169.0 17 11169.0 18 20111.5 19 29054.0 20 29054.0 21 29054.0 22 46253.0 23 63452.0 24 63452.0 25 63452.0 26 91566.0 27 119680.0 28 119680.0 29 119680.0 30 153841.5 31 188003.0 32 188003.0 33 188003.0 34 210476.0 35 232949.0 36 232949.0 37 232949.0 38 248260.0 39 263571.0 40 263571.0 41 263571.0 42 275633.0 43 287695.0 44 287695.0 45 287695.0 46 292751.5 47 297808.0 48 297808.0 49 297808.0 50 286686.0 51 275564.0 52 275564.0 53 275564.0 54 243185.5 55 210807.0 56 210807.0 57 210807.0 58 180009.0 59 149211.0 60 149211.0 61 149211.0 62 121810.5 63 94410.0 64 94410.0 65 94410.0 66 72662.0 67 50914.0 68 50914.0 69 50914.0 70 37174.5 71 23435.0 72 23435.0 73 23435.0 74 16433.5 75 9432.0 76 9432.0 77 9432.0 78 6367.5 79 3303.0 80 3303.0 81 3303.0 82 2196.0 83 1089.0 84 1089.0 85 1089.0 86 695.0 87 301.0 88 301.0 89 301.0 90 190.5 91 80.0 92 80.0 93 80.0 94 55.0 95 30.0 96 30.0 97 30.0 98 20.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.46955486509882 3 0.0 4 0.0 5 7.324911982564986E-4 6 0.001034105221067998 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.7235087017799966E-4 14 0.0 15 0.0 16 0.0 17 7.755789158009986E-4 18 0.0 19 5.601403280784989E-4 20 7.755789158009986E-4 21 9.048420684344984E-4 22 0.004136420884271992 23 4.308771754449992E-4 24 0.0021543858772249957 25 0.001034105221067998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2320847.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.27370118232244 #Duplication Level Percentage of deduplicated Percentage of total 1 83.1457505138668 59.26105376705275 2 11.56996073873917 16.492678487681964 3 2.8280716637957704 6.047014040627195 4 1.0111966320054413 2.8828690634450678 5 0.4950652224842367 1.7642565366550726 6 0.25330408701415086 1.0832351883664553 7 0.1489189769748093 0.7429804665695797 8 0.1037295439978836 0.591455081814698 9 0.07546656765642305 0.4840903433159474 >10 0.3294065195741384 4.078587372874338 >50 0.020253471331315425 1.0167975808276875 >100 0.015386417432200435 2.2279821884612376 >500 0.002203986396323295 1.1602566281588114 >1k 0.0012244368868455807 1.896729413794146 >5k 6.122184434227904E-5 0.27001384035516185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6188 0.2666267961653655 No Hit GTCCTACAGTGGACATTTCTAAATT 4192 0.18062371194654364 No Hit GGTATCAACGCAGAGTACTTTTTTT 4055 0.17472069464294718 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3904 0.16821444929372767 No Hit CTGTAGGACGTGGAATATGGCAAGA 3823 0.1647243441726232 No Hit TATCAACGCAGAGTACTTTTTTTTT 3762 0.16209599340240868 No Hit CTTTAGGACGTGAAATATGGCGAGG 3417 0.1472307308495562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 8.617543508899983E-5 0.0 0.0 0.0 5 0.0 1.2926315263349976E-4 0.0 0.0 0.0 6 0.0 1.2926315263349976E-4 0.0 0.0 0.0 7 0.0 1.2926315263349976E-4 0.0 0.0 0.0 8 0.0 1.2926315263349976E-4 0.0 0.0 0.0 9 0.0 1.2926315263349976E-4 0.0 0.0 0.0 10 0.0 1.2926315263349976E-4 0.0 4.3087717544499915E-5 0.0 11 0.0 1.2926315263349976E-4 0.0 4.3087717544499915E-5 0.0 12 0.0 1.2926315263349976E-4 0.0 8.617543508899983E-5 0.0 13 0.0 1.2926315263349976E-4 0.0 1.2926315263349976E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1340 0.0 13.86935 1 ACTGCGC 70 1.1519829E-4 12.143722 8 CGGTAGG 100 1.0506446E-6 11.990277 1 TAGGACC 865 0.0 10.919444 4 GTCCTAT 260 0.0 10.760506 1 TCCAACG 215 0.0 10.544066 18 GGCGAGG 1115 0.0 10.504664 19 ATAGGAC 270 0.0 10.144973 3 AGGACGT 2455 0.0 10.080137 5 CACCTTT 745 0.0 10.015627 14 GTATCAA 2610 0.0 9.953614 1 AGGACCT 1615 0.0 9.942441 5 GGACGTG 2455 0.0 9.810609 6 GATATAC 480 0.0 9.783733 1 CTAGACT 155 4.4450644E-8 9.750008 4 GTCCTAA 1325 0.0 9.65255 1 TTCCACC 735 0.0 9.637874 11 GGACCTG 1515 0.0 9.600976 6 TTAGGAC 1575 0.0 9.595244 3 CTAGGAC 365 0.0 9.574707 3 >>END_MODULE