FastQCFastQC Report
Thu 26 May 2016
SRR1511839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320847
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75910.32707886388029883No Hit
GGTATCAACGCAGAGTACTTTTTTT51480.22181556991908558No Hit
TATCAACGCAGAGTACTTTTTTTTT50070.21574020174531106No Hit
GTCCTACAGTGGACATTTCTAAATT45490.19600602710993015No Hit
CTGTAGGACGTGGAATATGGCAAGA43580.18777627305893063No Hit
GTCCTAAAGTGTGTATTTCTCATTT42350.18247648380095716No Hit
CTTTAGGACGTGAAATATGGCGAGG39560.17045501060604168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.013.299011
TAGGACC7250.012.1864974
ACCGCTC550.003076118912.0872918
TCCAACG1800.011.607635518
GGCGAGG9200.010.73591519
GTGGTAT4150.010.5734391
AGGACCG1355.6890713E-810.5558065
AAGCGTG909.547735E-510.5523967
AATCCCG2800.010.514708519
TATGTCG1451.45100785E-810.4796216
GTATCAA29000.010.4600951
CGCCCTA1502.4576366E-810.130300516
ACTGTTC6850.010.12105758
TGGCGAG22250.010.07338818
ACAACGT856.6137314E-410.0558138
AGTACCG951.6442205E-410.0002385
GATATAC6600.09.9726751
GTCCTAA13500.09.821721
AGGACGT25350.09.8187755
GTATTAC2053.8198777E-119.7717411
Produced by FastQC (version 0.11.5)