FastQCFastQC Report
Thu 26 May 2016
SRR1511838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745140
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60590.3471927753647272No Hit
GGTATCAACGCAGAGTACTTTTTTT42960.24616936177040238No Hit
TATCAACGCAGAGTACTTTTTTTTT39680.22737430807843495No Hit
GTCCTACAGTGGACATTTCTAAATT21410.12268356693445799No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19110.10950410855289547No Hit
CTGTAGGACGTGGAATATGGCAAGA18760.10749853879917944No Hit
GTCCTAAAGTGTGTATTTCTCATTT18620.106696310897693No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17720.10153913153099464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG505.6651712E-515.9861061
GGTATCA12550.014.7282351
TAGGACC4500.013.2235454
TAAGCCG658.4218016E-411.62509255
GATATAC2950.011.1767261
TCCAACG1201.3805948E-711.01993518
TCGGAAA700.001565027710.79472913
CGATGCT700.001565401710.794429
GCATTAG750.001741158210.6574051
GTCCTAA6150.010.3974681
TTAGGAC8400.010.2325053
GGCGAGG6400.010.18360119
GACAGTT1304.1588646E-710.1716647
GTATTAG1501.2703458E-79.9913171
GTCAACG800.00297636899.9913172
CCAACGA951.7420284E-49.94279919
CGAACCG1151.0991302E-59.85605712
CCCCGTA1252.7660935E-69.82320415
GTATCAA25050.09.7719461
CTTACAC2350.09.6463553
Produced by FastQC (version 0.11.5)