FastQCFastQC Report
Thu 26 May 2016
SRR1511838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745140
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79610.4561811659809528No Hit
GGTATCAACGCAGAGTACTTTTTTT54140.3102329899033889No Hit
TATCAACGCAGAGTACTTTTTTTTT54030.3096026679807924No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25530.14629198803534385No Hit
GTCCTACAGTGGACATTTCTAAATT23080.13225299975933164No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21250.12176673504704492No Hit
GTCCTAAAGTGTGTATTTCTCATTT20480.11735448158886966No Hit
CTGTAGGACGTGGAATATGGCAAGA20480.11735448158886966No Hit
CTTTAGGACGTGAAATATGGCGAGG18200.10428962719323377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.8321261
TAGGACC4750.012.9981774
AATCCCG1800.012.13579619
ATGTCGA550.003074569612.08782817
GTATTGG1552.910383E-1011.078011
CTAGACT2200.010.793944
TCCAACG1451.449007E-810.48008718
TGTAGGA13800.010.4623012
TGGACGG1002.4025516E-510.4485355
GTGGTAT3850.010.4066151
AGGACCT9500.010.1985695
GTAGGAC13300.010.0700163
GCGATTT856.609276E-410.0562611
GTATAGT1356.087157E-79.8927081
GTATCAA25100.09.8814471
ATTAGAC1252.5742847E-69.8786143
CTTACCC1651.024091E-89.7865063
TAGAAAT4150.09.613114
TGGACCG800.00452114649.4986685
CTAAAGC1606.647133E-89.4986683
Produced by FastQC (version 0.11.5)