FastQCFastQC Report
Thu 26 May 2016
SRR1511837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490463
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58320.23417332439791316No Hit
GGTATCAACGCAGAGTACTTTTTTT36340.14591664280898775No Hit
TATCAACGCAGAGTACTTTTTTTTT35680.14326653317073973No Hit
GTCCTACAGTGGACATTTCTAAATT29360.11788972572569839No Hit
GTCCTAAAGTGTGTATTTCTCATTT28530.11455701208971986No Hit
CTGTAGGACGTGGAATATGGCAAGA27540.1105818476323479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC602.7150043E-514.1669738
GCGTATA450.00822047813.3284741
GGTATCA12150.013.1639251
TAGGACC5750.012.3193554
GGACCGT855.650961E-511.1115746
GTCCTAA9850.011.0619571
GTATCAA26150.011.0475981
CTGCGTA600.00613854211.0187579
CCGAACA600.00613854211.0187579
TATGTCG901.0094074E-410.49468616
GTAGGAC18950.010.4665763
TGTAGGA19050.010.4423872
GACGTGG9550.010.3841697
GGCGAGG7150.010.03967219
GGACGTG19250.010.0090756
TAGGACA6900.09.9923654
GTCCTAC16450.09.8444351
AGGACGT19600.09.8303425
AGGACCG1252.7699243E-69.8226325
AATCCCG1356.9768976E-79.7950419
Produced by FastQC (version 0.11.5)