##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511837_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2490463 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.074777661824328 31.0 30.0 34.0 23.0 34.0 2 26.343472278046292 31.0 25.0 34.0 7.0 34.0 3 28.08359610241148 31.0 27.0 34.0 16.0 34.0 4 32.430613504396575 35.0 32.0 37.0 25.0 37.0 5 32.78322544844071 35.0 33.0 37.0 26.0 37.0 6 32.978236978425294 36.0 35.0 37.0 26.0 37.0 7 32.94571451171931 37.0 35.0 37.0 26.0 37.0 8 32.962669591959404 37.0 35.0 37.0 26.0 37.0 9 34.474763527906255 39.0 35.0 39.0 27.0 39.0 10 34.34301814562192 39.0 35.0 39.0 26.0 39.0 11 34.34231426044073 39.0 35.0 39.0 26.0 39.0 12 34.21377470775514 39.0 35.0 39.0 25.0 39.0 13 34.19883411237188 39.0 35.0 39.0 25.0 39.0 14 35.25195515853879 40.0 36.0 41.0 25.0 41.0 15 35.18002194772619 40.0 35.0 41.0 25.0 41.0 16 35.12858412271132 40.0 35.0 41.0 25.0 41.0 17 35.06952241410533 40.0 35.0 41.0 25.0 41.0 18 35.0366188937559 40.0 35.0 41.0 24.0 41.0 19 35.05690548303669 40.0 35.0 41.0 24.0 41.0 20 34.98466108510747 40.0 35.0 41.0 23.0 41.0 21 34.86736201260569 40.0 35.0 41.0 22.0 41.0 22 34.757390493253666 39.0 35.0 41.0 19.0 41.0 23 34.641931640823415 39.0 35.0 41.0 19.0 41.0 24 34.51019629683316 39.0 34.0 41.0 18.0 41.0 25 34.39478080983335 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8703485778059452 1101 2 3.4207872775302164 1101 3 2.0194465471027137 1101 4 1.1033506107777953 1101 5 0.4642553503710971 1101 6 0.5263479491588399 1101 7 0.27356820240661506 1101 8 0.3253734998753117 1101 9 0.3525948040103657 1101 10 0.2687196006645607 1101 11 0.3293938243619863 1101 12 0.3159840102667104 1101 13 0.28671209497201744 1101 14 0.2825011091311893 1101 15 0.464598166088976 1101 16 0.33243052892726865 1101 17 0.40827215209417744 1101 18 0.5231311099482312 1101 19 0.6136265804594672 1101 20 0.40172232568297517 1101 21 0.5865416264170236 1101 22 0.5617773470563705 1101 23 0.47654739676104896 1101 24 0.511660960145278 1101 25 0.5906479678495344 1102 1 1.7134213657038337 1102 2 4.345114850685771 1102 3 2.6606295981667607 1102 4 1.8153700951016418 1102 5 1.519792170999878 1102 6 1.3235078208949815 1102 7 1.3689112194229054 1102 8 1.1570290284655727 1102 9 1.3953069716413964 1102 10 1.4271425746663269 1102 11 1.1640612360939429 1102 12 1.2602659048551317 1102 13 1.3605391856761315 1102 14 1.5667426532356998 1102 15 1.43002453728527 1102 16 1.546858805021614 1102 17 1.559397817969213 1102 18 1.5074344901405397 1102 19 1.676449879777536 1102 20 1.7121199934553246 1102 21 1.737930880799425 1102 22 1.7326296995892108 1102 23 1.9155808044926133 1102 24 1.622797845317507 1102 25 1.7569892099210307 1103 1 1.356503796606578 1103 2 3.8666281210917646 1103 3 2.2778475652088552 1103 4 1.487715772643746 1103 5 1.042098826088818 1103 6 1.025854896715991 1103 7 1.0563583091445707 1103 8 0.8002757212074343 1103 9 0.779730596354014 1103 10 1.120284006368415 1103 11 1.2190051786614404 1103 12 0.9951743997177402 1103 13 1.0325863173256309 1103 14 1.2192079257163186 1103 15 0.9622185231254861 1103 16 0.9907164696740907 1103 17 1.2211841285107354 1103 18 1.2515381574298274 1103 19 1.34013156661522 1103 20 1.3982774224125905 1103 21 1.4436882377278764 1103 22 1.4110707233264819 1103 23 1.5660585087198768 1103 24 1.5053789655134366 1103 25 1.4223658736111346 1104 1 1.392751289181735 1104 2 3.892326219385371 1104 3 2.2302504228043993 1104 4 1.606692921970918 1104 5 1.3471995913380965 1104 6 1.110754431857039 1104 7 0.8436287485959895 1104 8 1.008689281337432 1104 9 1.225983992679005 1104 10 1.2516411639926446 1104 11 1.0303372762714105 1104 12 1.2361208216664181 1104 13 1.18885093803965 1104 14 1.403625795760398 1104 15 1.3799850994198195 1104 16 1.4186707938517955 1104 17 1.3644471489099885 1104 18 1.3013599552836226 1104 19 1.2436803544224304 1104 20 1.2125007278724524 1104 21 1.3341718131502915 1104 22 1.3194689498062644 1104 23 1.3714281527651835 1104 24 1.3451281847439986 1104 25 1.454221777351151 1105 1 1.4419453602460486 1105 2 4.079192368119006 1105 3 2.439162960404257 1105 4 1.7910346864886648 1105 5 1.327362249698325 1105 6 1.2025792125421333 1105 7 1.1234575924572852 1105 8 1.1067495180183826 1105 9 1.200113040730848 1105 10 1.1022012576370344 1105 11 1.2453616487629944 1105 12 1.1755432916310795 1105 13 1.2546438154756103 1105 14 1.309594218915997 1105 15 1.4356755626800606 1105 16 1.3855941759676327 1105 17 1.373706163261474 1105 18 1.5215537158882597 1105 19 1.5215464553208875 1105 20 1.454270404309689 1105 21 1.6393833149778771 1105 22 1.6019684972479737 1105 23 1.609021161700987 1105 24 1.5748842909981136 1105 25 1.5291984459781247 1106 1 1.1356123217930936 1106 2 3.74710097724817 1106 3 2.1193201288911894 1106 4 1.2522933985503713 1106 5 0.9046001024179731 1106 6 0.8027958256067151 1106 7 0.8161332598652891 1106 8 0.7067067328042924 1106 9 0.8283120105786352 1106 10 0.918070851611553 1106 11 0.894289951492155 1106 12 0.909110875734001 1106 13 0.71682313306178 1106 14 0.7769546812443409 1106 15 0.9021222403151725 1106 16 0.9924693671575966 1106 17 0.8592145838876704 1106 18 0.8968069134112682 1106 19 0.7238698784687259 1106 20 0.8634762528624336 1106 21 0.8753237215820917 1106 22 0.6085867704615353 1106 23 0.7074792404588521 1106 24 0.7915531623779088 1106 25 0.7614882610302516 1107 1 0.9411093772901502 1107 2 3.463825818973014 1107 3 1.9805612401322996 1107 4 1.239048010304984 1107 5 0.8399412877591601 1107 6 0.6212132690241603 1107 7 0.6182275619595856 1107 8 0.6734965745941324 1107 9 0.6948148770815052 1107 10 0.681236614777319 1107 11 0.5692408764430752 1107 12 0.6381077297308551 1107 13 0.6924789837176277 1107 14 0.9639127432023997 1107 15 0.6840551472480811 1107 16 0.7014039510921819 1107 17 0.643589868262957 1107 18 0.5005811421854958 1107 19 0.731102760701603 1107 20 0.7055764699626508 1107 21 0.6455322417906117 1107 22 0.8263987382735039 1107 23 0.8066498396294506 1107 24 0.8193065651313134 1107 25 0.8771656267076153 1108 1 1.3228226294561125 1108 2 3.9381605840313014 1108 3 2.3632945282819193 1108 4 1.7159185752919583 1108 5 1.2168115805550812 1108 6 0.9813196548985346 1108 7 1.1049500822310208 1108 8 1.171532035644951 1108 9 1.143082925389976 1108 10 1.1412861127209126 1108 11 1.0329540660050966 1108 12 1.0932373743309896 1108 13 1.2118099925393366 1108 14 1.293956987858607 1108 15 1.2452151166538101 1108 16 1.2942796223833781 1108 17 1.312206783848346 1108 18 1.3318393589530118 1108 19 1.338342634513829 1108 20 1.2745510445960733 1108 21 1.2820353760706311 1108 22 1.0599434279620894 1108 23 1.4561004219822138 1108 24 1.4216997369464721 1108 25 1.480063372693479 1109 1 0.4632565645686597 1109 2 2.4767508685013553 1109 3 1.3393576189208396 1109 4 0.7462565124174461 1109 5 0.32001710142964157 1109 6 0.27601474649321744 1109 7 0.22739449590112315 1109 8 0.2844192734235378 1109 9 0.24542188799819797 1109 10 0.242922828805753 1109 11 0.2896771416166999 1109 12 0.4209035106156165 1109 13 0.29242922439699726 1109 14 0.3664225861767676 1109 15 0.3297331476889198 1109 16 0.15860169621623044 1109 17 0.169124846953558 1109 18 0.2249559169816706 1109 19 0.15502016550885855 1109 20 0.11611792264444176 1109 21 0.24394515704006636 1109 22 0.32714743786579703 1109 23 0.13967102231742956 1109 24 0.09645327725450414 1109 25 0.23607881451143697 1110 1 0.8045623536557471 1110 2 3.3317368058645833 1110 3 1.8058497236732123 1110 4 0.9393284849543875 1110 5 0.7232899850884422 1110 6 0.6343091142073831 1110 7 0.34108134248327104 1110 8 0.4941219219185129 1110 9 0.37141093474552633 1110 10 0.4284065340346146 1110 11 0.47114323334543684 1110 12 0.40592685954027274 1110 13 0.3711877542710553 1110 14 0.5033673213463032 1110 15 0.5922843649918619 1110 16 0.6115886459370756 1110 17 0.6225575384927637 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-1.754262134092457 2315 16 -1.8146088905020505 2315 17 -1.6903698843014467 2315 18 -1.687931895164752 2315 19 -1.7428127510046068 2315 20 -1.7618105788197482 2315 21 -1.8296948351806464 2315 22 -1.761290166275863 2315 23 -1.6902278520156884 2315 24 -1.8980413324491465 2315 25 -1.9305530327552276 2316 1 -1.6145276380242848 2316 2 -1.347445496330561 2316 3 -1.098074580439004 2316 4 -1.2777327000241137 2316 5 -1.359254975953231 2316 6 -1.5164673001080224 2316 7 -1.606438453029007 2316 8 -1.729919304628197 2316 9 -1.729020248366524 2316 10 -1.8559769184039538 2316 11 -1.8981887966317572 2316 12 -1.8163426884292448 2316 13 -1.7135707610417938 2316 14 -2.096543974512379 2316 15 -2.487553411457249 2316 16 -2.4058513980986476 2316 17 -2.3536948064410765 2316 18 -2.2706391250025177 2316 19 -2.3484291172173712 2316 20 -2.4482738613070367 2316 21 -2.4044568969082007 2316 22 -2.316454525547499 2316 23 -2.2708455443175453 2316 24 -2.192708749303357 2316 25 -2.2791828038343596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112258.0 3 1.0 4 995.0 5 5092.0 6 6035.0 7 5231.0 8 4962.0 9 4593.0 10 4599.0 11 4414.0 12 4290.0 13 4202.0 14 4220.0 15 4477.0 16 4640.0 17 4937.0 18 5661.0 19 6877.0 20 8912.0 21 11724.0 22 14395.0 23 17013.0 24 19520.0 25 22014.0 26 24626.0 27 27845.0 28 31553.0 29 37993.0 30 47258.0 31 59370.0 32 74514.0 33 97511.0 34 129773.0 35 183231.0 36 277896.0 37 370987.0 38 846844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.550215361561285 18.6994948328885 12.774050447647687 24.97623935790253 2 16.299184858018425 21.10861179378408 39.47176623728744 23.120437110910053 3 18.43247621024685 24.91392162822736 29.719253006368696 26.934349155157093 4 12.261776223939084 17.018160880125503 38.54002247774812 32.1800404181873 5 13.190191808786286 37.87401388263723 35.812176840401065 13.123617468175416 6 31.505527546180666 37.250585841194074 18.386097855957754 12.857788756667507 7 27.81438632093711 31.747992240800205 22.55343685089881 17.884184587363876 8 24.93090642181795 36.668362469147304 20.908200603662852 17.492530505371892 9 26.428258520604402 15.279247272495114 20.443347281208354 37.84914692569213 10 14.870046252443823 29.019624061871223 34.643678705525836 21.46665098015911 11 33.98986453522899 22.535729300134154 24.791896125338944 18.682510039297913 12 23.006886671273573 24.84726735550779 31.870218509570307 20.275627463648327 13 28.670136709739047 22.075970734711955 26.00556764837325 23.248324907175746 14 21.001958270409958 22.096252785124694 28.05269542249774 28.8490935219676 15 22.97147156974426 30.62567080900218 23.73317732485887 22.669680296394688 16 22.224261111287337 28.267715681782867 27.493040450711376 22.01498275621842 17 21.72669681659046 28.538617426698785 27.791882809604555 21.942802947106195 18 21.517966739517913 27.604987506339185 29.57237268732762 21.304673066815287 19 23.262101226685942 27.0673171515188 27.82581461181714 21.844767009978117 20 23.3953771313962 27.281068242824073 28.25948776222723 21.064066863552497 21 23.11459019289764 26.762660413420917 27.50004015354716 22.622709240134274 22 23.067119130825507 27.88440881911593 27.589913598941195 21.45855845111737 23 22.380827905605926 27.536917982391156 27.90070722073454 22.181546891268376 24 22.816098669419617 26.92272017095932 28.449733518523203 21.811447641097857 25 22.64915223883953 27.31081470678699 28.034099971009162 22.00593308336432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 224.0 2 404.5 3 585.0 4 585.0 5 585.0 6 1642.5 7 2700.0 8 2700.0 9 2700.0 10 3933.5 11 5167.0 12 5167.0 13 5167.0 14 8622.0 15 12077.0 16 12077.0 17 12077.0 18 21596.0 19 31115.0 20 31115.0 21 31115.0 22 48406.5 23 65698.0 24 65698.0 25 65698.0 26 92991.5 27 120285.0 28 120285.0 29 120285.0 30 153696.5 31 187108.0 32 187108.0 33 187108.0 34 212869.0 35 238630.0 36 238630.0 37 238630.0 38 258245.5 39 277861.0 40 277861.0 41 277861.0 42 292249.0 43 306637.0 44 306637.0 45 306637.0 46 314082.0 47 321527.0 48 321527.0 49 321527.0 50 311873.5 51 302220.0 52 302220.0 53 302220.0 54 270660.0 55 239100.0 56 239100.0 57 239100.0 58 204965.5 59 170831.0 60 170831.0 61 170831.0 62 139420.5 63 108010.0 64 108010.0 65 108010.0 66 83218.5 67 58427.0 68 58427.0 69 58427.0 70 42459.0 71 26491.0 72 26491.0 73 26491.0 74 18499.0 75 10507.0 76 10507.0 77 10507.0 78 7116.0 79 3725.0 80 3725.0 81 3725.0 82 2423.0 83 1121.0 84 1121.0 85 1121.0 86 720.0 87 319.0 88 319.0 89 319.0 90 189.5 91 60.0 92 60.0 93 60.0 94 43.5 95 27.0 96 27.0 97 27.0 98 19.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.516082752484177 3 0.0 4 0.0 5 5.621444687192703E-4 6 0.0010841357611014498 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.6061270534836294E-4 14 0.0 15 0.0 16 0.0 17 8.030635267418147E-4 18 0.0 19 5.219912923821795E-4 20 7.227571740676332E-4 21 9.235230557530868E-4 22 0.004015317633709074 23 4.0153176337090734E-4 24 0.0018470461115061736 25 8.432167030789055E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2490463.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.69207825247757 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22328694281366 61.328969738182195 2 11.6748667629491 17.20690390164989 3 2.871579849584558 6.348380609514692 4 0.961365146259472 2.8337998234943025 5 0.4566811716796722 1.6826892319925777 6 0.23136864335681698 1.0230021702852081 7 0.1371645684996515 0.7075559480740548 8 0.09233560882213086 0.5443522328648501 9 0.060550795581140446 0.4015902569593658 >10 0.2616253098520191 3.298084304304432 >50 0.015322156248043242 0.7833166311842992 >100 0.011704968666573314 1.7397503676547774 >500 0.0012668138668118062 0.6598514614533636 >1k 8.261829566044989E-4 1.2050396776081287 >5k 5.507886377363326E-5 0.2367136447778843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5832 0.23417332439791316 No Hit GGTATCAACGCAGAGTACTTTTTTT 3634 0.14591664280898775 No Hit TATCAACGCAGAGTACTTTTTTTTT 3568 0.14326653317073973 No Hit GTCCTACAGTGGACATTTCTAAATT 2936 0.11788972572569839 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2853 0.11455701208971986 No Hit CTGTAGGACGTGGAATATGGCAAGA 2754 0.1105818476323479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0153176337090735E-5 2 0.0 0.0 0.0 0.0 4.0153176337090735E-5 3 0.0 0.0 0.0 0.0 4.0153176337090735E-5 4 0.0 0.0 0.0 0.0 4.0153176337090735E-5 5 0.0 0.0 0.0 0.0 4.0153176337090735E-5 6 0.0 0.0 0.0 0.0 4.0153176337090735E-5 7 0.0 0.0 0.0 0.0 4.0153176337090735E-5 8 4.0153176337090735E-5 0.0 0.0 0.0 4.0153176337090735E-5 9 4.0153176337090735E-5 0.0 0.0 4.0153176337090735E-5 4.0153176337090735E-5 10 4.0153176337090735E-5 0.0 0.0 4.0153176337090735E-5 4.0153176337090735E-5 11 4.0153176337090735E-5 0.0 0.0 8.030635267418147E-5 4.0153176337090735E-5 12 4.0153176337090735E-5 0.0 0.0 8.030635267418147E-5 4.0153176337090735E-5 13 4.0153176337090735E-5 0.0 0.0 8.030635267418147E-5 8.030635267418147E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 60 2.7150043E-5 14.166973 8 GCGTATA 45 0.008220478 13.328474 1 GGTATCA 1215 0.0 13.163925 1 TAGGACC 575 0.0 12.319355 4 GGACCGT 85 5.650961E-5 11.111574 6 GTCCTAA 985 0.0 11.061957 1 GTATCAA 2615 0.0 11.047598 1 CTGCGTA 60 0.006138542 11.018757 9 CCGAACA 60 0.006138542 11.018757 9 TATGTCG 90 1.0094074E-4 10.494686 16 GTAGGAC 1895 0.0 10.466576 3 TGTAGGA 1905 0.0 10.442387 2 GACGTGG 955 0.0 10.384169 7 GGCGAGG 715 0.0 10.039672 19 GGACGTG 1925 0.0 10.009075 6 TAGGACA 690 0.0 9.992365 4 GTCCTAC 1645 0.0 9.844435 1 AGGACGT 1960 0.0 9.830342 5 AGGACCG 125 2.7699243E-6 9.822632 5 AATCCCG 135 6.9768976E-7 9.79504 19 >>END_MODULE