FastQCFastQC Report
Thu 26 May 2016
SRR1511837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490463
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79240.318173769295107No Hit
GGTATCAACGCAGAGTACTTTTTTT51490.20674870495968017No Hit
TATCAACGCAGAGTACTTTTTTTTT50300.2019704769755664No Hit
GTCCTACAGTGGACATTTCTAAATT33620.13499497884529904No Hit
CTGTAGGACGTGGAATATGGCAAGA31490.12644235228549872No Hit
GTCCTAAAGTGTGTATTTCTCATTT31400.1260809736984649No Hit
CTTTAGGACGTGAAATATGGCGAGG28220.11331226362327004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.012.8009251
GCGCCAC1405.2750693E-1112.21094213
GGCGAGG9300.012.15234419
GTGCGCC1657.2759576E-1211.51199911
TAGGACC6550.011.3126064
TACTCCG855.327393E-511.1761045
TAGAACG600.005878775411.0829714
GACGTGA9650.011.0226687
GTACCGT1053.4805744E-610.8539536
TTAGGAC13050.010.7735933
AGGACGT18550.010.7543645
GGACGTG18400.010.6843616
TGGCGAG19650.010.4396818
TAGGACG19250.010.36329654
ACGTGAA11200.010.3451748
TGTAGGA17850.010.3245912
GTAGGAC17700.010.3047483
GGGACCG856.5979036E-410.0584945
CTTTAGG13300.010.0412111
CGTGAAA11550.010.0316849
Produced by FastQC (version 0.11.5)