FastQCFastQC Report
Thu 26 May 2016
SRR1511836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2898369
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50750.17509847779906562No Hit
GTCCTACAGTGGACATTTCTAAATT43980.15174051337148584No Hit
CTGTAGGACGTGGAATATGGCAAGA39390.13590402050256542No Hit
GTCCTAAAGTGTGTATTTCTCATTT30470.1051280910056656No Hit
GGTATCAACGCAGAGTACTTTTTTT29140.10053930331162111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9050.012.6281484
GGTATCA14050.012.090811
TACGAGT600.006137162411.0191894
GTATCAA30400.010.7815961
TGTAGGA25900.010.7257552
CTGTAGG25850.010.66918851
GTAGGAC25600.010.4780683
GGCGAGG9000.010.28564219
GTCCTAC23500.010.205311
GATCTAC1801.7498678E-99.99271
GCGTACA1209.338459E-69.99271
ACATCGC951.7444229E-49.9419558
TATGTCG1054.372956E-59.89580716
TGGTATC5700.09.8173892
CTTACAC4150.09.78640653
GATATAC5650.09.72740651
TGGCGAG21300.09.66773518
TCCAACG2253.6379788E-129.65590918
GAACAGT4900.09.6377756
AATCCCG2650.09.62422419
Produced by FastQC (version 0.11.5)