FastQCFastQC Report
Thu 26 May 2016
SRR1511836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2898369
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66210.22843882197194354No Hit
GTCCTACAGTGGACATTTCTAAATT48940.16885358627559155No Hit
CTGTAGGACGTGGAATATGGCAAGA43180.1489803403224365No Hit
GGTATCAACGCAGAGTACTTTTTTT40410.1394232411401033No Hit
TATCAACGCAGAGTACTTTTTTTTT38400.13248830635436687No Hit
GTCCTAAAGTGTGTATTTCTCATTT34270.11823891298864983No Hit
CTTTAGGACGTGAAATATGGCGAGG31680.10930285274235267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9300.014.3003614
GGTATCA17450.012.5137131
TATGCCG802.8698325E-511.8744075
AATATCG600.00587992111.0827815
TGCTCGC600.005888988711.08048613
GCACCGT1408.394636E-910.8543536
GGCGAGG10250.010.74882719
GACGTGA10900.010.6302967
TCCAACG1803.6379788E-1110.55320618
TGTAGGA28200.010.4764272
GTGGTAT6750.010.4537971
GTAGGAC26150.010.4258653
AGGACCT17800.010.4067845
AGGACGT22750.010.1467465
GGACCGA750.002654405510.130736
CTGTAGG27600.010.0883451
TTAGGAC13850.010.0825283
GGACGTG22550.010.0661496
TGGTATC6350.010.0231212
GTATCAA34200.010.0095471
Produced by FastQC (version 0.11.5)