FastQCFastQC Report
Thu 26 May 2016
SRR1511835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732376
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57440.33156774279948464No Hit
GTCCTACAGTGGACATTTCTAAATT41750.24099848993521036No Hit
CTGTAGGACGTGGAATATGGCAAGA38540.22246902520007203No Hit
GGTATCAACGCAGAGTACTTTTTTT37150.2144453629004327No Hit
TATCAACGCAGAGTACTTTTTTTTT32190.18581416505423765No Hit
GTCCTAAAGTGTGTATTTCTCATTT29220.16867008086004423No Hit
CTTTAGGACGTGAAATATGGCGAGG25040.1445413697719202No Hit
GTCCTACAGTGTGCATTTCTCATTT21600.12468424868504298No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17380.10032464084009475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG551.2728761E-414.5387231
TAGGACC6150.013.668254
AGACCGG500.001566831513.22288110
GGTATCA9900.013.1252361
TCGAACT1501.8189894E-1212.59358119
GGACGTT550.003205953612.0204536
CTCGAAC1501.4551915E-1111.96390218
AAGTCGT1603.6379788E-1211.8061437
AGGACCT11800.011.6860815
GCCTCGA1302.8649083E-911.624845516
ACGAAAT1157.6686774E-811.49815712
GTCCTAC20700.011.4439771
GGCGAGG6950.011.41576519
TATGTCG752.1885603E-411.33422416
GGACCTG11500.011.33357056
CAAGTCG1501.9463187E-1011.333576
AGTCGTC1605.0931703E-1111.2158368
TCCAACG1105.354632E-711.16249318
GATATAC4550.010.9839261
CCTCGAA1553.45608E-1010.96860417
Produced by FastQC (version 0.11.5)