##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1732376 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.520591372773577 31.0 30.0 34.0 25.0 34.0 2 26.748709864371246 31.0 26.0 34.0 10.0 34.0 3 28.516853731522488 31.0 28.0 34.0 16.0 34.0 4 32.89933536368548 35.0 33.0 37.0 27.0 37.0 5 33.27999926113038 35.0 35.0 37.0 28.0 37.0 6 33.49149664968806 37.0 35.0 37.0 28.0 37.0 7 33.47894914268034 37.0 35.0 37.0 28.0 37.0 8 33.4974699487871 37.0 35.0 37.0 28.0 37.0 9 35.04512242146047 39.0 35.0 39.0 28.0 39.0 10 34.92172715392039 39.0 35.0 39.0 28.0 39.0 11 34.92333650431546 39.0 35.0 39.0 27.0 39.0 12 34.808988926191546 39.0 35.0 39.0 27.0 39.0 13 34.80483740250384 39.0 35.0 39.0 27.0 39.0 14 35.897212845248376 40.0 36.0 41.0 27.0 41.0 15 35.84302830332445 40.0 36.0 41.0 27.0 41.0 16 35.79562462190656 40.0 36.0 41.0 27.0 41.0 17 35.75988238119207 40.0 36.0 41.0 27.0 41.0 18 35.72857566717618 40.0 36.0 41.0 27.0 41.0 19 35.76080596821937 40.0 36.0 41.0 27.0 41.0 20 35.6961773887424 40.0 36.0 41.0 27.0 41.0 21 35.59338792502321 40.0 36.0 41.0 27.0 41.0 22 35.49843740619819 40.0 36.0 41.0 27.0 41.0 23 35.383501041344374 40.0 36.0 41.0 26.0 41.0 24 35.27658372085506 40.0 36.0 41.0 25.0 41.0 25 35.173363634684385 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7098755407223969 1101 2 3.1925069593851063 1101 3 1.8485072261034539 1101 4 0.7393754254751244 1101 5 0.23152120483690197 1101 6 0.18740471116612412 1101 7 0.09151241887418138 1101 8 -0.14344824942586598 1101 9 -0.11453744403620902 1101 10 -0.09854176742017273 1101 11 -0.01841247179412875 1101 12 -0.082237581642147 1101 13 -0.26583108923009036 1101 14 -0.13629951358853276 1101 15 0.03490934954891145 1101 16 0.08787420668009815 1101 17 -0.012989142364943973 1101 18 0.10056463036323038 1101 19 0.30168896061604755 1101 20 0.038672788213332865 1101 21 0.18901924337529863 1101 22 0.036373095173495074 1101 23 -0.04939441318005322 1101 24 0.1632261195621112 1101 25 0.1854377112358918 1102 1 1.1178422191322532 1102 2 3.777695803820407 1102 3 2.023218413409648 1102 4 1.1044557733060643 1102 5 0.8641025380493161 1102 6 0.7005743781595086 1102 7 0.5894204452858887 1102 8 0.537758171424457 1102 9 0.5401718073878001 1102 10 0.5704031942284331 1102 11 0.5914575624936589 1102 12 0.5370964748856153 1102 13 0.5225987882337932 1102 14 0.6670833097299536 1102 15 0.5509976641454628 1102 16 0.7235385630777529 1102 17 0.8747283827753591 1102 18 0.7117178406139928 1102 19 0.7653384124649065 1102 20 0.7188118578039422 1102 21 0.6332688884635616 1102 22 0.6991132056059826 1102 23 0.9203186228064268 1102 24 0.5495083014234154 1102 25 0.8383850482642643 1103 1 1.3291312970004832 1103 2 3.9677499252177775 1103 3 2.495232573409915 1103 4 1.6033873002389782 1103 5 1.0247303563466872 1103 6 0.9650374347154411 1103 7 1.1424649630354864 1103 8 0.8256868636672294 1103 9 0.8750712818100155 1103 10 1.0822732490727915 1103 11 1.2910468182445882 1103 12 1.070672253248489 1103 13 1.199317172023271 1103 14 1.4497482379211633 1103 15 1.194371870146398 1103 16 1.1371267495149553 1103 17 1.1959171872624452 1103 18 1.2366563046356163 1103 19 1.3592677873198937 1103 20 1.3481744392214168 1103 21 1.4330201385182022 1103 22 1.391622881550127 1103 23 1.3286451468306808 1103 24 1.472448359327764 1103 25 1.4928046261207228 1104 1 1.3238840185502276 1104 2 3.827264047298897 1104 3 2.352321496305585 1104 4 1.5844281172397956 1104 5 1.193190840387274 1104 6 1.022747021645877 1104 7 0.8962599021496516 1104 8 1.0270094212027487 1104 9 1.1366313710657252 1104 10 1.1384988671379688 1104 11 1.095160913410382 1104 12 1.1062202177170448 1104 13 1.1337469419778756 1104 14 1.1991694376836008 1104 15 1.1970501055651823 1104 16 1.2048510109352293 1104 17 1.209540045620983 1104 18 1.2353133591488117 1104 19 1.2413339651592352 1104 20 1.2231721224087764 1104 21 1.196004966820226 1104 22 1.3304392844224893 1104 23 1.4005126751098942 1104 24 1.331106670963642 1104 25 1.4245154653387644 1105 1 1.0301952464822222 1105 2 3.5907475483841687 1105 3 2.077174732437431 1105 4 1.2439603656042522 1105 5 0.6879407694094297 1105 6 0.7219100936280967 1105 7 0.5397618422107726 1105 8 0.5166023899694494 1105 9 0.630654731172207 1105 10 0.5490690710962411 1105 11 0.5738431371997166 1105 12 0.3680635963235588 1105 13 0.5241815706187367 1105 14 0.5219721009555442 1105 15 0.6282084640078835 1105 16 0.5652826172936258 1105 17 0.6833438781193735 1105 18 0.6602073503017678 1105 19 0.8073044383754038 1105 20 0.7776136565071425 1105 21 0.9251489282082801 1105 22 0.8554089852775846 1105 23 0.7804509778082718 1105 24 0.7460233139710297 1105 25 0.7579258821389914 1106 1 1.1260273299405448 1106 2 3.8759440287361357 1106 3 2.231934256485026 1106 4 1.3524511831891601 1106 5 1.0913904244439223 1106 6 0.9450644448596393 1106 7 0.8450146502633018 1106 8 0.7229084260325109 1106 9 0.662448218490141 1106 10 0.8255814198613649 1106 11 0.7923146233844349 1106 12 0.6423428350663514 1106 13 0.5483608193605249 1106 14 0.7431949786677734 1106 15 0.7572355612981525 1106 16 0.8659792143005873 1106 17 0.6368401951543348 1106 18 0.7222661444223561 1106 19 0.7238677820410331 1106 20 0.8071823663028255 1106 21 0.9131351295881416 1106 22 0.7759635964831304 1106 23 0.7137059856408214 1106 24 0.9289083587998803 1106 25 1.1232143532918712 1107 1 1.013438107515011 1107 2 3.4447050527540704 1107 3 2.0129086560366467 1107 4 1.258488636002241 1107 5 0.80798126235171 1107 6 0.6437679379279047 1107 7 0.5851692749933619 1107 8 0.49777448936600877 1107 9 0.6028872010660109 1107 10 0.512030326113937 1107 11 0.585832912951382 1107 12 0.5482418179719062 1107 13 0.6119836207386342 1107 14 0.7447140265223098 1107 15 0.778254139344078 1107 16 0.8843380162062005 1107 17 0.7965133072655064 1107 18 0.688378931458935 1107 19 0.7101543158425301 1107 20 0.758480521685847 1107 21 0.5474666001715249 1107 22 0.6125501484175828 1107 23 0.5846289535125067 1107 24 0.6920737149477816 1107 25 0.7730668169023147 1108 1 1.049543311675702 1108 2 3.659049051567184 1108 3 2.0521397359370788 1108 4 1.3038210462028559 1108 5 0.839221474672236 1108 6 0.6327356777363562 1108 7 0.614991819213067 1108 8 0.6240499785439226 1108 9 0.5319687664353481 1108 10 0.5174763970303147 1108 11 0.6392324316036095 1108 12 0.6013610999065335 1108 13 0.7014430111413503 1108 14 0.8434232891700546 1108 15 0.7168246023940412 1108 16 0.8584449677252479 1108 17 0.7892374554283066 1108 18 0.6772889754725853 1108 19 0.588251343684874 1108 20 0.7628901288599081 1108 21 0.7178154948849453 1108 22 0.8161462448849548 1108 23 0.6567425153211914 1108 24 0.7420362126811568 1108 25 0.9160225724699558 1109 1 0.7682677412866319 1109 2 2.8810142255039075 1109 3 1.7012788285286682 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-0.18174105183106093 2314 6 -0.1683733064646944 2314 7 -0.25184948670970897 2314 8 -0.2737569777813107 2314 9 -0.41574663305818405 2314 10 -0.4060188140987435 2314 11 -0.38848469600434754 2314 12 -0.4496290764276054 2314 13 -0.30738449645238575 2314 14 -1.2489809832605374 2314 15 -0.7313053783973018 2314 16 -0.7714843125150921 2314 17 -0.6657443329042607 2314 18 -0.5554531433286414 2314 19 -0.4748746823830672 2314 20 -0.36892512241458064 2314 21 -0.45140097138691715 2314 22 -0.31819305044315627 2314 23 -0.6252915750591939 2314 24 -0.5424554558133252 2314 25 -0.41247453293837566 2315 1 -4.393176969725182 2315 2 -13.60872858055796 2315 3 -7.141681133172181 2315 4 -3.320869259886152 2315 5 -2.104503263699222 2315 6 -1.5214984443059905 2315 7 -1.2760183182101912 2315 8 -1.033642665269845 2315 9 -1.0917465773412758 2315 10 -1.3135317740823993 2315 11 -1.3293082382528496 2315 12 -1.1934604027916862 2315 13 -1.1520160568274491 2315 14 -1.2910024389110433 2315 15 -1.4112477578340474 2315 16 -1.478033370086905 2315 17 -1.2452827647984748 2315 18 -1.2291004414070983 2315 19 -1.3826669233401034 2315 20 -1.3385605713651287 2315 21 -1.475483209534886 2315 22 -1.4077914012228447 2315 23 -1.4489341139855796 2315 24 -1.4220321930779463 2315 25 -1.522423714480297 2316 1 -1.789579440331405 2316 2 -1.4892172257631167 2316 3 -1.2196257019683436 2316 4 -1.3392502689832213 2316 5 -1.4867848553099314 2316 6 -1.711384428612572 2316 7 -1.7862165280018019 2316 8 -1.850834233082601 2316 9 -1.7622553698062475 2316 10 -1.7840510622886114 2316 11 -1.9476178306758385 2316 12 -2.046505879013125 2316 13 -2.007055267547713 2316 14 -2.4243989748600967 2316 15 -2.7862913950190915 2316 16 -2.510318954642649 2316 17 -2.4760722498911036 2316 18 -2.4207816538021163 2316 19 -2.3598382298089007 2316 20 -2.4973577137854903 2316 21 -2.510943299478825 2316 22 -2.506086755944672 2316 23 -2.5806114018860384 2316 24 -2.575809404998374 2316 25 -2.6611808361829787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62004.0 3 1.0 4 532.0 5 2840.0 6 3394.0 7 3106.0 8 2867.0 9 2751.0 10 2587.0 11 2513.0 12 2575.0 13 2462.0 14 2525.0 15 2657.0 16 2820.0 17 2953.0 18 3450.0 19 4253.0 20 5507.0 21 6972.0 22 8528.0 23 10223.0 24 11934.0 25 13733.0 26 15215.0 27 17670.0 28 20329.0 29 24486.0 30 30426.0 31 38906.0 32 49777.0 33 65104.0 34 89389.0 35 127044.0 36 196915.0 37 265614.0 38 628314.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9900899111971 19.310299842528412 12.563669780694262 25.13594046558022 2 15.607377808783705 21.148859198405827 41.299956077199056 21.94380691561141 3 19.121945813149107 24.60458930393864 29.824703182219103 26.44876170069315 4 11.624208601365984 16.740649835832404 39.80411873634823 31.83102282645338 5 12.178243883516666 38.46653828747703 36.76153103728538 12.593686791720929 6 30.717109695057083 38.312772713707396 18.43580739997587 12.534310191259653 7 27.24876123889964 32.019261407454266 22.638734316337793 18.093243037308298 8 23.764702350990778 38.824712418089376 20.929232453000964 16.481352777918882 9 25.798960502800778 15.72528134769819 21.079430793315076 37.39632735618596 10 14.055089657210676 29.753413808549645 35.789632273825084 20.401864260414598 11 33.67490660226187 22.870670108567655 25.310152068604047 18.144271220566434 12 22.74864117258609 25.219178746415327 32.8138925960646 19.218287484933988 13 28.608429819426984 22.671951899532086 26.18562735298498 22.53399092805595 14 19.593783335719266 23.332059552891522 28.68390003094017 28.39025708044905 15 21.866904182463852 32.5634850632888 24.003565045925367 21.566045708321983 16 20.577288071411747 29.447879675082085 29.446667467108757 20.52816478639741 17 20.447203936355177 29.698550651017914 29.072758381033086 20.781487031593823 18 20.017074815167145 28.29068285406863 31.722847695881264 19.96939463488296 19 22.148758231507394 27.777873985204067 28.91816288456032 21.155204898728215 20 22.237378107525714 28.227852412335263 29.923162932565674 19.61160654757335 21 21.567238661270558 27.72716278785171 28.585241280819968 22.120357270057763 22 21.60672671341331 29.460427854717118 28.95699805520583 19.97584737666374 23 20.73279915214466 28.933987003938533 29.420891945201117 20.912321898715692 24 21.66478290133726 27.54761462202124 30.535008595280623 20.252593881360884 25 21.28015747258805 28.46080358354042 29.808729684564167 20.450309259307364 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 359.0 3 554.0 4 554.0 5 554.0 6 1557.5 7 2561.0 8 2561.0 9 2561.0 10 3942.5 11 5324.0 12 5324.0 13 5324.0 14 8125.5 15 10927.0 16 10927.0 17 10927.0 18 18537.5 19 26148.0 20 26148.0 21 26148.0 22 40155.0 23 54162.0 24 54162.0 25 54162.0 26 75858.0 27 97554.0 28 97554.0 29 97554.0 30 125299.5 31 153045.0 32 153045.0 33 153045.0 34 171714.5 35 190384.0 36 190384.0 37 190384.0 38 200758.5 39 211133.0 40 211133.0 41 211133.0 42 217517.5 43 223902.0 44 223902.0 45 223902.0 46 223906.0 47 223910.0 48 223910.0 49 223910.0 50 210740.5 51 197571.0 52 197571.0 53 197571.0 54 169534.5 55 141498.0 56 141498.0 57 141498.0 58 117668.0 59 93838.0 60 93838.0 61 93838.0 62 74366.5 63 54895.0 64 54895.0 65 54895.0 66 41059.5 67 27224.0 68 27224.0 69 27224.0 70 19386.0 71 11548.0 72 11548.0 73 11548.0 74 7816.0 75 4084.0 76 4084.0 77 4084.0 78 2746.5 79 1409.0 80 1409.0 81 1409.0 82 890.5 83 372.0 84 372.0 85 372.0 86 244.0 87 116.0 88 116.0 89 116.0 90 74.0 91 32.0 92 32.0 93 32.0 94 22.5 95 13.0 96 13.0 97 13.0 98 10.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.507638064715743 3 0.0 4 0.0 5 4.6179351364830727E-4 6 0.0010967595949147298 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.1544837841207682E-4 14 0.0 15 0.0 16 0.0 17 6.92690270472461E-4 18 0.0 19 4.6179351364830727E-4 20 5.195177028543457E-4 21 9.81311216502653E-4 22 0.003405727163156266 23 2.886209460301921E-4 24 0.0017317256761811522 25 6.349660812664226E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1732376.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.10113048375325 #Duplication Level Percentage of deduplicated Percentage of total 1 87.94873150123207 68.68895354858306 2 9.13299984778226 14.265952256394815 3 1.6610156707864052 3.8918160491894387 4 0.5219661545938257 1.630645869921412 5 0.2202280476638713 0.8600029743389119 6 0.11527222306277927 0.540173456074706 7 0.07392515069394845 0.40415464882654323 8 0.05552837942184487 0.3469463365421491 9 0.03764007402717511 0.26457590997130814 >10 0.19904108623281003 2.7802051711994076 >50 0.017560202802615804 0.9382940597114333 >100 0.012948609083289202 2.0163751401220074 >500 0.0017960300669142223 0.9765267577483442 >1k 0.0012721879640642405 2.059660219239385 >5k 7.48345861214259E-5 0.33571760213708934 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5744 0.33156774279948464 No Hit GTCCTACAGTGGACATTTCTAAATT 4175 0.24099848993521036 No Hit CTGTAGGACGTGGAATATGGCAAGA 3854 0.22246902520007203 No Hit GGTATCAACGCAGAGTACTTTTTTT 3715 0.2144453629004327 No Hit TATCAACGCAGAGTACTTTTTTTTT 3219 0.18581416505423765 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2922 0.16867008086004423 No Hit CTTTAGGACGTGAAATATGGCGAGG 2504 0.1445413697719202 No Hit GTCCTACAGTGTGCATTTCTCATTT 2160 0.12468424868504298 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1738 0.10032464084009475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 55 1.2728761E-4 14.538723 1 TAGGACC 615 0.0 13.66825 4 AGACCGG 50 0.0015668315 13.222881 10 GGTATCA 990 0.0 13.125236 1 TCGAACT 150 1.8189894E-12 12.593581 19 GGACGTT 55 0.0032059536 12.020453 6 CTCGAAC 150 1.4551915E-11 11.963902 18 AAGTCGT 160 3.6379788E-12 11.806143 7 AGGACCT 1180 0.0 11.686081 5 GCCTCGA 130 2.8649083E-9 11.6248455 16 ACGAAAT 115 7.6686774E-8 11.498157 12 GTCCTAC 2070 0.0 11.443977 1 GGCGAGG 695 0.0 11.415765 19 TATGTCG 75 2.1885603E-4 11.334224 16 GGACCTG 1150 0.0 11.3335705 6 CAAGTCG 150 1.9463187E-10 11.33357 6 AGTCGTC 160 5.0931703E-11 11.215836 8 TCCAACG 110 5.354632E-7 11.162493 18 GATATAC 455 0.0 10.983926 1 CCTCGAA 155 3.45608E-10 10.968604 17 >>END_MODULE