FastQCFastQC Report
Thu 26 May 2016
SRR1511835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732376
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74310.4289484499900714No Hit
GGTATCAACGCAGAGTACTTTTTTT50980.29427791657238384No Hit
GTCCTACAGTGGACATTTCTAAATT45810.26443451075286195No Hit
TATCAACGCAGAGTACTTTTTTTTT43050.24850263453199536No Hit
CTGTAGGACGTGGAATATGGCAAGA41210.2378813837180843No Hit
GTCCTAAAGTGTGTATTTCTCATTT33120.1911825146503992No Hit
CTTTAGGACGTGAAATATGGCGAGG28610.1651489053184759No Hit
GTCCTACAGTGTGCATTTCTCATTT24550.1417128845008243No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24310.14032750395987936No Hit
CTGTAGGACCTGGAATATGGCGAGA18200.10505802435498991No Hit
CTGAAGGACCTGGAATATGGCGAGA17430.10061326178612494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGT400.00527942514.254
GGTATCA10850.013.0096061
TCCAACG1403.6379788E-1212.88987918
TAGGACC7000.012.7571434
CTGCTCG550.003075330312.08741959
GCGTGCG550.003075330312.08741959
CCAACGA802.8735873E-511.87225819
ACGGCAA600.00588875911.08013417
GTAGGAC22600.011.0553093
GTCGAGG700.001494428510.85463519
TGTCGAG803.7791996E-410.68503218
TGTAGGA24150.010.6604562
AGGACCT13700.010.6094895
TAGGACA7550.010.5695364
CTGTAGG24050.010.5486961
GGCGAGG7950.010.51329519
GTCCTAC21500.010.2472121
GGACCTG13000.010.2278176
AGTGCGC750.002654181610.1304088
GTATCAA23850.010.1172931
Produced by FastQC (version 0.11.5)