FastQCFastQC Report
Thu 26 May 2016
SRR1511834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261250
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62620.27692647871752346No Hit
GTCCTACAGTGGACATTTCTAAATT49890.22063018242122723No Hit
GTCCTAAAGTGTGTATTTCTCATTT47290.20913211719181868No Hit
CTGTAGGACGTGGAATATGGCAAGA44220.19555555555555554No Hit
CTTTAGGACGTGAAATATGGCGAGG43760.19352128247650635No Hit
GGTATCAACGCAGAGTACTTTTTTT42110.1862244333886125No Hit
TATCAACGCAGAGTACTTTTTTTTT39690.1755223880597015No Hit
GTCCTACAGTGTGCATTTCTCATTT28910.1278496406854616No Hit
CTGTAGGACCTGGAATATGGCGAGA22710.10043117744610282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG806.9519956E-814.9873861
GGTATCA11100.013.6821781
GTCAACG450.00824609413.3221222
GCGTGCG701.1518288E-412.1438679
TAGGACC9350.011.8191654
GACGGGA803.042463E-511.8065377
CGTGCGC658.4246777E-411.62489710
GGCGAGG13750.011.40347219
AGTACCG752.1890303E-411.3342755
TAAGACC1605.0931703E-1111.2162094
GTCCTAA15550.011.1160461
TGCCGGT1201.38225E-711.01943410
ACTACGC700.001565557610.7945483
AATGTCC9250.010.7216128
CGTAGGA750.001741123310.6576982
AGGACCT20050.010.5993845
GTATCAA23550.010.521931
TAGAACG901.0093002E-410.49469954
CACCTTT7950.010.45509714
TCCAACG1451.5790647E-810.42278418
Produced by FastQC (version 0.11.5)