FastQCFastQC Report
Thu 26 May 2016
SRR1511834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261250
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79900.3533443891652847No Hit
GTCCTACAGTGGACATTTCTAAATT56150.24831398562741844No Hit
GGTATCAACGCAGAGTACTTTTTTT56100.24809286898839136No Hit
TATCAACGCAGAGTACTTTTTTTTT54070.23911553344389164No Hit
GTCCTAAAGTGTGTATTTCTCATTT52390.23168601437258154No Hit
CTTTAGGACGTGAAATATGGCGAGG50910.22514096185737978No Hit
CTGTAGGACGTGGAATATGGCAAGA49820.2203206191265893No Hit
GTCCTACAGTGTGCATTTCTCATTT32450.14350469872857932No Hit
CTGTAGGACCTGGAATATGGCGAGA25790.11405196241017136No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25730.11378662244333886No Hit
CTGAAGGACCTGGAATATGGCGAGA24010.10618021006080708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.014.1756431
TCCAACG1450.013.7550518
TAGGACC10600.012.3677234
CCAACGA853.956042E-612.29119919
AATCCCG1658.0035534E-1110.93679719
GCACCGT700.001495642710.8538456
AAATGTC8300.010.7557697
ATGTCGA1252.2507265E-710.63676917
TGTAGGA32550.010.5359262
GGCGAGG13250.010.39375719
GAAATGT8900.010.2440796
TATGTCG1303.8592952E-710.22766116
GTATAAG2800.010.2215861
AATGTCC9050.010.0742878
CTGTAGG33100.010.0301261
ATGTCCA8950.09.9746239
TAGAAAT10050.09.9252124
GTCCTAA16500.09.8870631
GGACGTG28400.09.831526
AGGACGT28800.09.7967155
Produced by FastQC (version 0.11.5)