FastQCFastQC Report
Thu 26 May 2016
SRR1511833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313007
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62580.27055689844431946No Hit
GTCCTACAGTGGACATTTCTAAATT58020.2508423018175042No Hit
CTGTAGGACGTGGAATATGGCAAGA53350.23065213378083163No Hit
GTCCTAAAGTGTGTATTTCTCATTT50680.21910871865065693No Hit
CTTTAGGACGTGAAATATGGCGAGG45750.19779447273613956No Hit
GGTATCAACGCAGAGTACTTTTTTT41520.17950659033889652No Hit
TATCAACGCAGAGTACTTTTTTTTT39940.17267565554276318No Hit
GTCCTACAGTGTGCATTTCTCATTT32510.14055296849512344No Hit
CTGAAGGACCTGGAATATGGCGAGA25020.10817087886028878No Hit
CTGTAGGACCTGGAATATGGCGAGA23160.10012939865724575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA454.5992917E-415.5479572
GGTATCA12750.013.6403931
AAACCCG707.715149E-613.4928665
TCCAACG2350.013.26319818
CAGGACG655.7600126E-513.0777014
TAGGACC10750.012.7397744
CCAACGA1650.012.5933419
GACTACG550.00320610612.0206577
CCCGTTT1657.2759576E-1211.44849112
CGTGGAC1002.070341E-611.3340063
CGTTTCC2000.011.33400614
ATCCCGT1605.0931703E-1111.21570110
GGCGAGG13950.010.76527519
CACCTTT8500.010.66729914
TGTAGGA33100.010.6292472
CCGTTTC1701.546141E-1010.55618313
GTCGAGG901.00956604E-410.4944519
ACCTTTT9100.010.48291815
GTCCTAC30300.010.3249871
GTATCAA24900.010.3162441
Produced by FastQC (version 0.11.5)