##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511833_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2313007 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.2713169480248 31.0 30.0 34.0 24.0 34.0 2 26.544839250378402 31.0 26.0 34.0 10.0 34.0 3 28.27803158399434 31.0 28.0 34.0 16.0 34.0 4 32.63086017465576 35.0 32.0 37.0 26.0 37.0 5 33.000909206068116 35.0 35.0 37.0 27.0 37.0 6 33.20194707581948 37.0 35.0 37.0 27.0 37.0 7 33.18047459432678 37.0 35.0 37.0 26.0 37.0 8 33.197364729116686 37.0 35.0 37.0 27.0 37.0 9 34.72360265230498 39.0 35.0 39.0 27.0 39.0 10 34.59167136113293 39.0 35.0 39.0 27.0 39.0 11 34.58530821566904 39.0 35.0 39.0 27.0 39.0 12 34.47077202965664 39.0 35.0 39.0 27.0 39.0 13 34.46381744629394 39.0 35.0 39.0 27.0 39.0 14 35.527761481050426 40.0 36.0 41.0 27.0 41.0 15 35.475277420258564 40.0 36.0 41.0 26.0 41.0 16 35.42143841328625 40.0 36.0 41.0 26.0 41.0 17 35.38025349685496 40.0 36.0 41.0 26.0 41.0 18 35.351315408902785 40.0 36.0 41.0 26.0 41.0 19 35.38619511311466 40.0 36.0 41.0 25.0 41.0 20 35.322339707575466 40.0 36.0 41.0 25.0 41.0 21 35.20854454828714 40.0 36.0 41.0 25.0 41.0 22 35.10486652223707 40.0 36.0 41.0 24.0 41.0 23 34.988488145517934 40.0 35.0 41.0 24.0 41.0 24 34.87452005117149 39.0 35.0 41.0 23.0 41.0 25 34.775157619497044 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.864925174603524 1101 2 3.3815808456290384 1101 3 2.0453251012581823 1101 4 1.1067749411992125 1101 5 0.5629907673283796 1101 6 0.6615573728468576 1101 7 0.3925374684505627 1101 8 0.5321281374861115 1101 9 0.5154170265936457 1101 10 0.48493610723338065 1101 11 0.5474246853864528 1101 12 0.4368528009536945 1101 13 0.5452963905860884 1101 14 0.4745997678382494 1101 15 0.5412262092352762 1101 16 0.5160056823722385 1101 17 0.49927022211917915 1101 18 0.7424813323549628 1101 19 0.7071269970476166 1101 20 0.5381313998750272 1101 21 0.47898734431485224 1101 22 0.7019158674951029 1101 23 0.6046339352814698 1101 24 0.6713878444861194 1101 25 0.7619359527450698 1102 1 1.2084105083188632 1102 2 3.8852097222214965 1102 3 2.183309333879226 1102 4 1.4350440501019293 1102 5 0.9817559878021811 1102 6 0.8592935132624859 1102 7 0.747161214696284 1102 8 0.6824324862622788 1102 9 0.7010156358399229 1102 10 0.7818542572007274 1102 11 0.6289578651088377 1102 12 0.7504281888860902 1102 13 0.9982805139474991 1102 14 0.9337451632731373 1102 15 0.9161887574612635 1102 16 0.9518226894838264 1102 17 1.0976830438584457 1102 18 0.9855417674996616 1102 19 1.061092029109254 1102 20 0.9612274293799814 1102 21 0.9042964883748894 1102 22 1.0226387777832358 1102 23 1.1021498568379045 1102 24 0.9991691698297416 1102 25 1.045068207888157 1103 1 1.3349902106804663 1103 2 3.878614615023622 1103 3 2.4438585846011804 1103 4 1.6860719813784542 1103 5 1.2955291833870675 1103 6 1.0973670136464264 1103 7 1.128766332682254 1103 8 0.9693847783794354 1103 9 0.9827090843090929 1103 10 1.3040089556757906 1103 11 1.4238556608510748 1103 12 1.2150432328628895 1103 13 1.1101832232200763 1103 14 1.4384266788829052 1103 15 1.2491244858462025 1103 16 1.1747203062096148 1103 17 1.3145440375881563 1103 18 1.5296283360841585 1103 19 1.5041968338164438 1103 20 1.3697608607012697 1103 21 1.703705263007322 1103 22 1.5298333545271134 1103 23 1.6642871718738732 1103 24 1.68565837466695 1103 25 1.543733122078038 1104 1 1.2283985682108103 1104 2 3.795202271047195 1104 3 2.3310399158227746 1104 4 1.6163796560054422 1104 5 1.284535480894462 1104 6 1.019010004055609 1104 7 0.9320127722651463 1104 8 0.9594972248901215 1104 9 1.0400012279576245 1104 10 1.057886313200747 1104 11 1.0554977174981133 1104 12 1.077632950345972 1104 13 1.0451691359815527 1104 14 1.19129261039145 1104 15 1.2049575363573481 1104 16 1.2516946295463072 1104 17 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1107 18 0.9838726500055728 1107 19 1.0710379722142847 1107 20 1.1439079812705515 1107 21 0.9920801994787283 1107 22 1.0737627823512952 1107 23 1.0997293955859817 1107 24 1.1307944790117475 1107 25 1.2145773598945695 1108 1 0.8810625359325961 1108 2 3.5973975073867486 1108 3 1.9240265373099952 1108 4 1.2509557316283235 1108 5 0.8395219025886149 1108 6 0.7862356366233243 1108 7 0.682978338451818 1108 8 0.5999363042533048 1108 9 0.6014249581583542 1108 10 0.6623494014318467 1108 11 0.7142587838474626 1108 12 0.4952291428839004 1108 13 0.5787060870671539 1108 14 0.6776224318334201 1108 15 0.7224949945482422 1108 16 0.6199583836740601 1108 17 0.722480671915072 1108 18 0.6326137288682219 1108 19 0.4702707847651908 1108 20 0.5812971688935349 1108 21 0.5540428853596495 1108 22 0.4904180910605831 1108 23 0.545183536202444 1108 24 0.6093109749106418 1108 25 0.6787244171361735 1109 1 0.5542415089723534 1109 2 2.584722964039134 1109 3 1.2901206911629863 1109 4 0.8667519381902977 1109 5 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2315 13 -1.5579501909803497 2315 14 -1.6979447403758456 2315 15 -1.628432007657608 2315 16 -1.6971177285549786 2315 17 -1.365764825197715 2315 18 -1.540540370826264 2315 19 -1.452085425346091 2315 20 -1.5823893375468856 2315 21 -1.6959720937939764 2315 22 -1.6923861171137062 2315 23 -1.8109792563173812 2315 24 -1.9077895341347713 2315 25 -1.9958257396763628 2316 1 -1.6193364768868896 2316 2 -1.404659118688535 2316 3 -1.0806384925680845 2316 4 -1.3052574983903291 2316 5 -1.5919704083963495 2316 6 -1.5469355074991924 2316 7 -1.503408073770835 2316 8 -1.6373107900213384 2316 9 -1.7089404372610701 2316 10 -1.8490049073593866 2316 11 -1.8294488523914723 2316 12 -1.6984209747070764 2316 13 -1.6266170935559927 2316 14 -1.7790792810792126 2316 15 -2.3268813598152676 2316 16 -2.0496375691047106 2316 17 -1.9746345490027437 2316 18 -1.9527081323525337 2316 19 -2.0665511822509615 2316 20 -2.1608472187213508 2316 21 -2.140255989074582 2316 22 -2.1462651562987887 2316 23 -2.1102078642124695 2316 24 -2.088101871569293 2316 25 -2.178599998982669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95567.0 3 5.0 4 870.0 5 4249.0 6 5169.0 7 4696.0 8 4228.0 9 4125.0 10 3802.0 11 3799.0 12 3750.0 13 3725.0 14 3694.0 15 3831.0 16 3890.0 17 4237.0 18 4956.0 19 6014.0 20 7889.0 21 9958.0 22 12450.0 23 14839.0 24 17092.0 25 19374.0 26 21628.0 27 24347.0 28 28082.0 29 34061.0 30 42073.0 31 52831.0 32 67721.0 33 88325.0 34 119014.0 35 168775.0 36 260005.0 37 346511.0 38 817425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13502164066084 19.757138651115195 13.150932963021727 24.95690674520224 2 15.444811919174267 21.498278196111336 41.320731324020755 21.736178560693645 3 18.26838396943892 25.490411399533166 30.305269287987457 25.935935343040466 4 11.563605298211375 17.453038404120697 40.1421180307712 30.841238266896724 5 12.289055781837645 38.617669832982635 36.713989190628766 12.379285194550956 6 30.072711582577238 38.635367280393126 18.931038567662238 12.360882569367403 7 26.684787378507718 32.18433839586305 23.37861493717918 17.752259288450055 8 23.553063177067774 38.76088572148723 21.351210783192613 16.33484031825239 9 25.98370865284887 15.291350177496222 21.33551692666732 37.38942424298759 10 14.240164426653271 29.678682338618085 35.88303018538206 20.198123049346588 11 33.04408503735613 22.545846164754366 26.29512145877639 18.11494733911311 12 22.846796399665024 25.04994580647616 33.303919962196396 18.79933783166242 13 28.549311393619988 22.86973006975774 26.420435753489507 22.160522783132762 14 19.724237756305968 23.494697594948914 28.847556449245502 27.933508199499613 15 21.77801450665735 33.02769944059832 23.90606686447555 21.28821918826878 16 20.48791897300786 28.914957888151655 29.91266347226792 20.68445966657256 17 20.47847262303906 29.753370644427008 29.079479546992303 20.688677185541625 18 20.02298306922547 28.130697399532295 32.10937104816371 19.73694848307852 19 22.648926911708056 27.42454918877975 28.602501429962945 21.32402246954925 20 22.611703995005595 28.234041854086577 29.633789712700626 19.520464438207203 21 21.764424351086326 27.512158552102246 28.231144138235464 22.492272958575967 22 22.078097268130495 29.503325932763623 28.529779906912793 19.888796892193085 23 20.906648981128846 29.025452215541627 29.24745817230516 20.82044063102437 24 22.12204694791837 27.522864779376604 30.18479462987895 20.170293642826074 25 21.515953836296458 28.373799063202288 29.608480120502247 20.501766979999015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 409.0 3 637.0 4 637.0 5 637.0 6 1857.5 7 3078.0 8 3078.0 9 3078.0 10 4597.5 11 6117.0 12 6117.0 13 6117.0 14 10008.5 15 13900.0 16 13900.0 17 13900.0 18 24171.0 19 34442.0 20 34442.0 21 34442.0 22 53961.0 23 73480.0 24 73480.0 25 73480.0 26 104720.5 27 135961.0 28 135961.0 29 135961.0 30 175403.0 31 214845.0 32 214845.0 33 214845.0 34 237423.0 35 260001.0 36 260001.0 37 260001.0 38 272862.5 39 285724.0 40 285724.0 41 285724.0 42 292755.5 43 299787.0 44 299787.0 45 299787.0 46 298510.0 47 297233.0 48 297233.0 49 297233.0 50 276141.5 51 255050.0 52 255050.0 53 255050.0 54 218722.0 55 182394.0 56 182394.0 57 182394.0 58 151131.0 59 119868.0 60 119868.0 61 119868.0 62 94988.5 63 70109.0 64 70109.0 65 70109.0 66 52972.5 67 35836.0 68 35836.0 69 35836.0 70 25633.0 71 15430.0 72 15430.0 73 15430.0 74 10671.5 75 5913.0 76 5913.0 77 5913.0 78 4019.0 79 2125.0 80 2125.0 81 2125.0 82 1378.5 83 632.0 84 632.0 85 632.0 86 410.0 87 188.0 88 188.0 89 188.0 90 122.0 91 56.0 92 56.0 93 56.0 94 36.5 95 17.0 96 17.0 97 17.0 98 10.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.500113056294252 3 0.0 4 0.0 5 6.052727034548534E-4 6 0.0014267142295721544 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 8.646752906497905E-5 14 0.0 15 0.0 16 0.0 17 9.511428197147696E-4 18 0.0 19 4.323376453248952E-4 20 8.214415261173011E-4 21 0.0011240778778447277 22 0.005274519272963722 23 2.5940258719493714E-4 24 0.002637259636481861 25 9.0790905518228E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2313007.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.59992086690059 #Duplication Level Percentage of deduplicated Percentage of total 1 87.64057725936257 66.25620705537297 2 9.08635437716754 13.738553437649633 3 1.798397453729394 4.0787611556753305 4 0.5610317242313261 1.6965581582283618 5 0.2501488554663088 0.9455616839099348 6 0.14297331752897902 0.6485262894761438 7 0.09937710069830871 0.5259030664141905 8 0.06688268705908536 0.4045060679226023 9 0.05069758762217527 0.3449460251141288 >10 0.2645716119711968 3.646832879433775 >50 0.018754327880624942 0.9844688437841241 >100 0.01658539816362084 2.5248506470091456 >500 0.0017372852946390823 0.9239672676630413 >1k 0.0016793757848144984 2.296937534477167 >5k 2.3163803928475838E-4 0.983419887869489 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6258 0.27055689844431946 No Hit GTCCTACAGTGGACATTTCTAAATT 5802 0.2508423018175042 No Hit CTGTAGGACGTGGAATATGGCAAGA 5335 0.23065213378083163 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5068 0.21910871865065693 No Hit CTTTAGGACGTGAAATATGGCGAGG 4575 0.19779447273613956 No Hit GGTATCAACGCAGAGTACTTTTTTT 4152 0.17950659033889652 No Hit TATCAACGCAGAGTACTTTTTTTTT 3994 0.17267565554276318 No Hit GTCCTACAGTGTGCATTTCTCATTT 3251 0.14055296849512344 No Hit CTGAAGGACCTGGAATATGGCGAGA 2502 0.10817087886028878 No Hit CTGTAGGACCTGGAATATGGCGAGA 2316 0.10012939865724575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3233764532489526E-5 2 0.0 0.0 0.0 0.0 4.3233764532489526E-5 3 0.0 0.0 0.0 0.0 4.3233764532489526E-5 4 0.0 0.0 0.0 0.0 4.3233764532489526E-5 5 0.0 0.0 0.0 0.0 4.3233764532489526E-5 6 0.0 0.0 0.0 0.0 4.3233764532489526E-5 7 0.0 0.0 0.0 0.0 8.646752906497905E-5 8 0.0 0.0 0.0 0.0 8.646752906497905E-5 9 0.0 0.0 0.0 0.0 8.646752906497905E-5 10 0.0 0.0 0.0 4.3233764532489526E-5 8.646752906497905E-5 11 0.0 0.0 0.0 8.646752906497905E-5 8.646752906497905E-5 12 0.0 0.0 0.0 8.646752906497905E-5 8.646752906497905E-5 13 0.0 0.0 0.0 8.646752906497905E-5 8.646752906497905E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGGA 45 4.5992917E-4 15.547957 2 GGTATCA 1275 0.0 13.640393 1 AAACCCG 70 7.715149E-6 13.492866 5 TCCAACG 235 0.0 13.263198 18 CAGGACG 65 5.7600126E-5 13.077701 4 TAGGACC 1075 0.0 12.739774 4 CCAACGA 165 0.0 12.59334 19 GACTACG 55 0.003206106 12.020657 7 CCCGTTT 165 7.2759576E-12 11.448491 12 CGTGGAC 100 2.070341E-6 11.334006 3 CGTTTCC 200 0.0 11.334006 14 ATCCCGT 160 5.0931703E-11 11.215701 10 GGCGAGG 1395 0.0 10.765275 19 CACCTTT 850 0.0 10.667299 14 TGTAGGA 3310 0.0 10.629247 2 CCGTTTC 170 1.546141E-10 10.556183 13 GTCGAGG 90 1.00956604E-4 10.49445 19 ACCTTTT 910 0.0 10.482918 15 GTCCTAC 3030 0.0 10.324987 1 GTATCAA 2490 0.0 10.316244 1 >>END_MODULE