FastQCFastQC Report
Thu 26 May 2016
SRR1511833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313007
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78520.3394715191091077No Hit
GTCCTACAGTGGACATTTCTAAATT63750.27561524889462075No Hit
CTGTAGGACGTGGAATATGGCAAGA61580.26623352199107053No Hit
GTCCTAAAGTGTGTATTTCTCATTT56810.24561101630907298No Hit
GGTATCAACGCAGAGTACTTTTTTT53900.23302999083011852No Hit
TATCAACGCAGAGTACTTTTTTTTT53440.23104123766162404No Hit
CTTTAGGACGTGAAATATGGCGAGG52930.22883631567046708No Hit
GTCCTACAGTGTGCATTTCTCATTT33310.1440116696577226No Hit
CTGAAGGACCTGGAATATGGCGAGA28180.12183274845255548No Hit
CTGTAGGACCTGGAATATGGCGAGA26130.11296982672339513No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25280.10929495673813353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.014.16074851
TAGGACC10150.012.448224
TATACCG550.003069328812.0908555
TCCAACG2300.011.97535818
TATTGCG658.0452755E-411.68896916
AATGTCC8450.011.6889698
GGCGAGG12600.011.38216219
TGGCGAA1201.2804412E-711.08064718
ATTGCGA600.005889634611.08016917
TGTAGGA33950.010.9410392
AGGACCT19000.010.899955
GACGTGG15550.010.8714927
GGACCGT700.001495437510.8540436
GGCGAAC700.001495437510.85404310
GGACGTG31200.010.8062096
AGGACGT32350.010.6892865
ATGTCCA9650.010.6290889
CCAACGA1803.6379788E-1110.55299719
GGACCTG19050.010.4694516
ACACCGT1002.407338E-510.4470166
Produced by FastQC (version 0.11.5)