FastQCFastQC Report
Thu 26 May 2016
SRR1511832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245336
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66840.2976837319670642No Hit
GTCCTACAGTGGACATTTCTAAATT44420.19783230661246246No Hit
CTGTAGGACGTGGAATATGGCAAGA41450.18460488764265126No Hit
GTCCTAAAGTGTGTATTTCTCATTT41120.18313517442378335No Hit
GGTATCAACGCAGAGTACTTTTTTT40400.17992852740079882No Hit
TATCAACGCAGAGTACTTTTTTTTT39940.17787983624722536No Hit
CTTTAGGACGTGAAATATGGCGAGG37370.1664338878457389No Hit
GTCCTACAGTGTGCATTTCTCATTT23220.1034143664912512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG608.6583714E-716.654181
CGGTAGG651.9952004E-615.373091
TAGACGA400.005486206614.1679584
CCAACGA1450.013.02858719
GGTATCA12450.012.7615171
CCAACGT1155.802576E-912.32051219
TCCAACG2650.012.11904518
CGCCCTA1104.1334715E-812.02183316
TAGGACC9200.011.7039664
GTATCAA26850.011.0531651
CGTTAAA600.006137081411.0190339
TGTAGGA25000.010.7119682
CCGCCCT1502.3483153E-910.70444115
TACGGTG803.9787375E-410.6259695
ACACCGT803.979556E-410.6257316
ATCCCGT1356.197479E-810.49431610
CGGTTTC1901.0913936E-1110.43931513
GTAGGAC27600.010.4377473
GTCCCGC1451.5805199E-810.42217412
CGAAATC1554.0308805E-910.35913813
Produced by FastQC (version 0.11.5)