FastQCFastQC Report
Thu 26 May 2016
SRR1511832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245336
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84830.3778053707774694No Hit
GGTATCAACGCAGAGTACTTTTTTT56570.2519444751253265No Hit
TATCAACGCAGAGTACTTTTTTTTT55310.2463328428351035No Hit
GTCCTACAGTGGACATTTCTAAATT48910.21782931374190764No Hit
GTCCTAAAGTGTGTATTTCTCATTT45410.2022414462690662No Hit
CTGTAGGACGTGGAATATGGCAAGA43840.19524917428839159No Hit
CTTTAGGACGTGAAATATGGCGAGG42070.1873661670235546No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25890.11530568253481883No Hit
GTCCTACAGTGTGCATTTCTCATTT24910.11094107964242322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCAC456.7815033E-414.77273758
TACACCG851.7145794E-814.5315745
GGTATCA16100.013.1531991
TAGGACC7900.012.74873354
TCCAACG1700.012.29047118
CCAACGA1251.4188117E-912.15639419
CTGGACG802.8633249E-511.8767684
ACACCGT752.081182E-411.39611156
TGTAGGA27950.010.6062312
TAGCACT3000.010.4515554
GTGGTAT5600.010.3907321
GTAGGAC28200.010.3100463
AGGACCT16200.010.2638745
CTGTAGG27850.010.2411871
GTATTAC1409.128962E-810.2203921
AGGACGT27000.010.2052235
GGACGTG26350.010.1635156
AGCGTGT750.002655861210.1298778
TGGCGAA856.613385E-410.055840518
GGACCTG15700.010.041166
Produced by FastQC (version 0.11.5)